LOCUS       V047594                 7776 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047594
VERSION     V047594
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             603..1301
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric blue fluorescent protein"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
                     /label="TagBFP"
     CDS             1353..4409
                     /label="PLEKHM2(NM_015164)"
                     /note="PLEKHM2(NM_015164)"
                     /gene="PLEKHM2"
     CDS             4410..4427
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     polyA_signal    4564..4685
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4692..5147)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5174..5278
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5280..5637
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5672..6466
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6698..6745
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7074..7662
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
      661 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      721 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      781 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
      841 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
      901 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
      961 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     1021 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     1081 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
     1141 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     1201 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     1261 gatactgcga cctccctagc aaactggggc acaagcttaa tggcggcgga gggagtggag
     1321 gagggggaag cggaggcgga gggagtctcg agatggagcc gggggaggtg aaggaccgga
     1381 tcctggagaa catctcgctg tcggtgaaga agttgcagag ctattttgct gcatgtgagg
     1441 atgagatccc tgccatccgg aaccatgaca aggtcctaca gcgtctgtgt gagcacctgg
     1501 accacgccct gctgtacgga ctgcaagacc tctcctctgg ctactgggtg ctcgtggtgc
     1561 attttactcg gagagaggcc atcaagcaga tcgaggtgct gcagcacgtg gccaccaacc
     1621 tggggcgcag ccgtgcctgg ctgtacctgg ccctcaacga gaactccttg gagagctacc
     1681 tgcggttgtt ccaggagaac ctgggcctgc tgcataagta ctacgtcaag aatgccctgg
     1741 tctgcagcca cgatcacctg acgctcttcc tgaccttggt gtccgggcta gagttcattc
     1801 gtttcgagct ggatctggat gccccttacc tagacctggc cccctacatg cccgactact
     1861 acaaacctca gtacctgctg gactttgaag accgccttcc cagctcggtc cacggctcag
     1921 acagtctgtc cctcaactct ttcaactccg tcacctccac caacctggag tgggatgaca
     1981 gtgcgattgc cccatctagt gaggattatg attttggaga tgtgtttcca gcagtgccgt
     2041 ctgtacccag cacagactgg gaagatggag acctcacaga cacggtcagt ggtccccgct
     2101 ccacagcctc cgacctgacc agcagcaagg cctccaccag gagccccacc cagcgccaga
     2161 accccttcaa cgaggagccg gcagagactg tgtcctcctc tgacaccacc cccgtgcaca
     2221 ccacctctca ggagaaggag gaggcccagg ccctggaccc gccggatgcc tgcacggagc
     2281 tcgaggtcat cagggtcacc aagaagaaga aaattggcaa gaagaaaaag agcagatcag
     2341 atgaggaggc aagtccactc caccccgcct gcagccagaa gaaatgtgcc aagcaggggg
     2401 acggtgacag ccgcaacggc agcccaagcc ttgggcggga ctcgccagac actatgcttg
     2461 cctcccccca ggaggaggga gaggggccga gcagcaccac ggagagcagc gagcgctccg
     2521 agccgggcct gctgatccct gagatgaagg acacctccat ggagcgcttg gggcagcccc
     2581 tgagcaaggt tatcgaccag ctcaacgggc agctggaccc cagcacctgg tgctcccgtg
     2641 ctgagccccc agaccagtcc tttcggaccg gctctcccgg ggatgccccg gagaggccgc
     2701 cgctttgcga ctttagtgag gggctttcag ccccaatgga cttctaccgc tttaccgtcg
     2761 agagtccaag cactgttaca tcaggtggcg gccaccatga ccctgcaggg cttggccaac
     2821 cgctgcatgt tcctagtagc cctgaggctg ctggccaaga agaagaggga ggaggaggag
     2881 agggacagac gcctcggccc ctagaggata ccacgaggga ggctcaggag ctggaggccc
     2941 agctgtccct ggtcagggag gggcctgtgt ctgagccaga gcctgggacc caggaggttc
     3001 tctgccagct caagcgagac cagcccagcc cgtgtctgag tagcgctgag gattctgggg
     3061 tggatgaggg acaggggagc ccttcggaga tggtccattc ctcggagttc agagtagaca
     3121 acaatcacct gctcctgctc atgatccacg tgttccgaga aaacgaagag cagctgttca
     3181 aaatgatccg gatgagcacc gggcacatgg agggcaacct gcagctgctg tacgtgctgc
     3241 tcacagactg ctatgtctac ctgctccgga aaggggccac agagaagcca tacctggtgg
     3301 aagaggccgt ttcttacaat gaacttgact atgtgtcggt tggccttgac cagcagacgg
     3361 tgaagctggt gtgcaccaac cgcaggaagc agtttctgct ggacacggct gatgtggcgc
     3421 tggctgagtt ctttttggct tctttgaagt cagccatgat caaaggctgt cgagaacctc
     3481 cctaccccag catcctgacg gatgccacca tggagaagct ggcactggcc aaatttgtgg
     3541 cccaagaatc gaagtgtgag gcatctgctg tcaccgtgcg cttctacggc cttgtgcact
     3601 gggaggaccc cacagacgag tccctgggcc ccacgccctg ccactgctca ccccccgagg
     3661 gcaccatcac caaagaaggc atgctgcact acaaggcggg cacctcctac ctgggcaagg
     3721 aacactggaa gacgtgcttc gtggtgctca gcaacgggat cctctaccag tacccggacc
     3781 gcaccgacgt catccctctg ctctcggtga acatgggggg ggagcagtgc ggtggctgcc
     3841 ggagagccaa caccacggat cggccccacg ccttccaggt cattctctcc gaccggccct
     3901 gcctggagct aagtgccgag agcgaggccg agatggccga gtggatgcag catctctgcc
     3961 aggctgtgtc caaaggggtc atcccccagg gcgtagctcc cagcccctgc ataccctgct
     4021 gcctggtcct cacggatgac cgcctcttta cgtgccatga ggattgccag accagcttct
     4081 tccgctcttt gggcacagcc aagctgggcg acatcagcgc cgtctccacc gagccgggca
     4141 aggagtactg cgtcttggag ttctcccagg acagccagca gctcctcccg ccctgggtca
     4201 tctacctgag ctgcacttct gaactggacc gattgctgtc tgcactgaac tctgggtgga
     4261 aaaccatcta tcaggtggac ctcccccaca cggcgatcca ggaagcctcc aacaagaaga
     4321 aattcgagga tgccttgagc ctcatccaca gcgcctggca gcggagcgac agtctctgcc
     4381 gcggccgagc ctcccgagac ccctggtgcc accatcacca tcaccattag ggatccaccg
     4441 gtgcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     4501 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     4561 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4621 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4681 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4741 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4801 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4861 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4921 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4981 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     5041 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     5101 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     5161 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5221 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     5281 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5341 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5401 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5461 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5521 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5581 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     5641 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5701 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5761 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5821 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     5881 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5941 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6001 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6061 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6121 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6181 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6241 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6301 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6361 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6421 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6481 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6541 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6601 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     6661 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     6721 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     6781 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     6841 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     6901 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     6961 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7021 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7081 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7141 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7201 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7261 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7321 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7381 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7441 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7501 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7561 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7621 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7681 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7741 cctgattctg tggataaccg tattaccgcc atgcat
//
