LOCUS V018428 7686 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018428 VERSION V018428 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1369..4320 /label="PKN2(NM_006256)" /note="PKN2(NM_006256)" /gene="PKN2" polyA_signal 4474..4595 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4602..5057) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5084..5188 /gene="bla" /label="AmpR promoter" promoter 5190..5547 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5582..6376 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6608..6655 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6984..7572 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattcat ggcgtccaac 1381 cccgaacggg gggagattct gctcacggaa ctgcaggggg attcccgaag tcttccgttt 1441 tctgagaatg tgagtgctgt tcaaaaatta gacttttcag atacaatggt gcagcagaaa 1501 ttggatgata tcaaggatcg aattaagaga gaaataagga aagaactgaa aatcaaagaa 1561 ggagctgaaa atctgaggaa agtcacaaca gataaaaaaa gtttggctta tgtagacaac 1621 attttgaaaa aatcaaataa aaaattagaa gaactacatc acaagctgca ggaattaaat 1681 gcacatattg ttgtatcaga tccagaagat attacagatt gcccaaggac tccagatact 1741 ccaaataatg accctcgttg ttctactagc aacaatagat tgaaggcctt acaaaaacaa 1801 ttggatatag aacttaaagt aaaacaaggt gcagagaata tgatacagat gtattcaaat 1861 ggatcttcaa aggatcggaa actccatggt acagctcagc aactgctcca ggacagcaag 1921 acaaaaatag aagtcatacg aatgcagatt cttcaggcag tccagactaa tgaattggct 1981 tttgataatg caaaacctgt gataagtcct cttgaacttc ggatggaaga attaaggcat 2041 cattttagga tagagtttgc agtagcagaa ggtgcaaaga atgtaatgaa attacttggc 2101 tcaggaaaag taacagacag aaaagcactt tcagaagctc aagcaagatt taatgaatca 2161 agtcagaagt tggacctttt aaagtattca ttagagcaaa gattaaacga agtccccaag 2221 aatcatccca aaagcaggat tattattgaa gaactttcac ttgttgctgc atcaccaaca 2281 ctaagtccac gtcaaagtat gatatctacg caaaatcaat atagtacact atccaaacca 2341 gcagcactaa caggtacttt ggaagttcgt cttatgggct gccaagatat cctagagaat 2401 gtccctggac ggtcaaaagc aacatcagtt gcactgcctg gttggagtcc aagtgaaacc 2461 agatcatctt tcatgagcag aacgagtaaa agtaaaagcg gaagtagtcg aaatcttcta 2521 aaaaccgatg acttgtccaa tgatgtctgt gctgttttga agctcgataa tactgtggtt 2581 ggccaaacta gctggaaacc catttccaat cagtcatggg accagaagtt tacactggaa 2641 ctggacaggt cacgtgaact ggaaatttca gtttattggc gtgattggcg gtctctgtgt 2701 gctgtaaaat ttctgaggtt agaagatttt ttagacaacc aacggcatgg catgtgtctc 2761 tatttggaac cacagggtac tttatttgca gaggttacct tttttaatcc agttattgaa 2821 agaagaccaa aacttcaaag acaaaagaaa attttttcaa agcaacaagg caaaacattt 2881 ctcagagctc ctcaaatgaa tattaatatt gccacttggg gaaggctagt aagaagagct 2941 attcctacag taaatcattc tggcaccttc agccctcaag ctcctgtgcc tactacagtg 3001 ccagtggttg atgtacgcat ccctcaacta gcacctccag ctagtgattc tacagtaacc 3061 aaattggact ttgatcttga gcctgaacct cctccagccc caccacgagc ttcttctctt 3121 ggagaaatag atgaatcttc tgaattaaga gttttggata taccaggaca ggattcagag 3181 actgtttttg atattcagaa tgacagaaat agtatacttc caaaatctca atctgaatac 3241 aagcctgata ctcctcagtc aggcctagaa tatagtggta ttcaagaact tgaggacaga 3301 agatctcagc aaaggtttca gtttaatcta caagatttca ggtgttgtgc tgtcttggga 3361 agaggacatt ttggaaaggt gcttttagct gaatataaaa acacaaatga gatgtttgct 3421 ataaaagcct taaagaaagg agatattgtg gctcgagatg aagtagacag cctgatgtgt 3481 gaaaaaagaa tttttgaaac tgtgaatagt gtaaggcatc cctttttggt gaaccttttt 3541 gcatgtttcc aaaccaaaga gcatgtttgc tttgtaatgg aatatgctgc cggtggggac 3601 ctaatgatgc acattcatac tgatgtcttt tctgaaccaa gagctgtatt ttatgctgct 3661 tgtgtagttc ttgggttgca gtatttacat gaacacaaaa ttgtttatag agatttgaaa 3721 ttggataact tattgctaga tacagagggc tttgtgaaaa ttgctgattt tggtctttgc 3781 aaagaaggaa tgggatatgg agatagaaca agcacatttt gtggcactcc tgaatttctt 3841 gccccagaag tattaacaga aacttcttat acaagggctg tagattggtg gggccttggc 3901 gtgcttatat atgaaatgct tgttggtgag tctccctttc ctggtgatga tgaagaggaa 3961 gtttttgaca gtattgtaaa tgatgaagta aggtatccaa ggttcttatc tacagaagcc 4021 atttctataa tgagaaggct gttaagaaga aatcctgaac ggcgccttgg ggctagcgag 4081 aaagatgcag aggatgtaaa aaagcaccca tttttccggc taattgattg gagcgctctg 4141 atggacaaaa aagtaaagcc accatttata cctaccataa gaggacgaga agatgttagt 4201 aattttgatg atgaatttac ctcagaagca cctattctga ctccacctcg agaaccaagg 4261 atactttcgg aagaggagca ggaaatgttc agagattttg actacattgc tgattggtgt 4321 taagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc 4381 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 4441 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 4501 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 4561 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat 4621 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 4681 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 4741 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 4801 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 4861 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 4921 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 4981 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 5041 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 5101 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 5161 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 5221 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 5281 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 5341 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 5401 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 5461 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 5521 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 5581 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 5641 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 5701 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 5761 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 5821 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 5881 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 5941 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 6001 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 6061 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 6121 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 6181 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 6241 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 6301 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 6361 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 6421 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 6481 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 6541 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 6601 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 6661 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 6721 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 6781 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 6841 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 6901 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 6961 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 7021 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 7081 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 7141 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 7201 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 7261 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 7321 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 7381 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 7441 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 7501 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 7561 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 7621 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 7681 atgcat //