LOCUS V044377 6897 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044377 VERSION V044377 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="enhanced CFP" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNG IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="ECFP" CDS 1381..3552 /label="PIK3R1(NM_181523)" /note="PIK3R1(NM_181523)" /gene="PIK3R1" polyA_signal 3685..3806 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3813..4268) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4295..4399 /gene="bla" /label="AmpR promoter" promoter 4401..4758 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4793..5587 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5819..5866 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6195..6783 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac 1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgaggc ggtggaggcg gatcaggagg cggtggatct 1381 atgagtgctg aggggtacca gtacagagcg ctgtatgatt ataaaaagga aagagaagaa 1441 gatattgact tgcacttggg tgacatattg actgtgaata aagggtcctt agtagctctt 1501 ggattcagtg atggacagga agccaggcct gaagaaattg gctggttaaa tggctataat 1561 gaaaccacag gggaaagggg ggactttccg ggaacttacg tagaatatat tggaaggaaa 1621 aaaatctcgc ctcccacacc aaagccccgg ccacctcggc ctcttcctgt tgcaccaggt 1681 tcttcgaaaa ctgaagcaga tgttgaacaa caagctttga ctctcccgga tcttgcagag 1741 cagtttgccc ctcctgacat tgccccgcct cttcttatca agctcgtgga agccattgaa 1801 aagaaaggtc tggaatgttc aactctatac agaacacaga gctccagcaa cctggcagaa 1861 ttacgacagc ttcttgattg tgatacaccc tccgtggact tggaaatgat cgatgtgcac 1921 gttttggctg acgctttcaa acgctatctc ctggacttac caaatcctgt cattccagca 1981 gccgtttaca gtgaaatgat ttctttagct ccagaagtac aaagctccga agaatatatt 2041 cagctattga agaagcttat taggtcgcct agcatacctc atcagtattg gcttacgctt 2101 cagtatttgt taaaacattt cttcaagctc tctcaaacct ccagcaaaaa tctgttgaat 2161 gcaagagtac tctctgaaat tttcagccct atgcttttca gattctcagc agccagctct 2221 gataatactg aaaacctcat aaaagttata gaaattttaa tctcaactga atggaatgaa 2281 cgacagcctg caccagcact gcctcctaaa ccaccaaaac ctactactgt agccaacaac 2341 ggtatgaata acaatatgtc cttacaagat gctgaatggt actggggaga tatctcgagg 2401 gaagaagtga atgaaaaact tcgagataca gcagacggga cctttttggt acgagatgcg 2461 tctactaaaa tgcatggtga ttatactctt acactaagga aagggggaaa taacaaatta 2521 atcaaaatat ttcatcgaga tgggaaatat ggcttctctg acccattaac cttcagttct 2581 gtggttgaat taataaacca ctaccggaat gaatctctag ctcagtataa tcccaaattg 2641 gatgtgaaat tactttatcc agtatccaaa taccaacagg atcaagttgt caaagaagat 2701 aatattgaag ctgtagggaa aaaattacat gaatataaca ctcagtttca agaaaaaagt 2761 cgagaatatg atagattata tgaagaatat acccgcacat cccaggaaat ccaaatgaaa 2821 aggacagcta ttgaagcatt taatgaaacc ataaaaatat ttgaagaaca gtgccagacc 2881 caagagcggt acagcaaaga atacatagaa aagtttaaac gtgaaggcaa tgagaaagaa 2941 atacaaagga ttatgcataa ttatgataag ttgaagtctc gaatcagtga aattattgac 3001 agtagaagaa gattggaaga agacttgaag aagcaggcag ctgagtatcg agaaattgac 3061 aaacgtatga acagcattaa accagacctt atccagctga gaaagacgag agaccaatac 3121 ttgatgtggt tgactcaaaa aggtgttcgg caaaagaagt tgaacgagtg gttgggcaat 3181 gaaaacactg aagaccaata ttcactggtg gaagatgatg aagatttgcc ccatcatgat 3241 gagaagacat ggaatgttgg aagcagcaac cgaaacaaag ctgaaaacct gttgcgaggg 3301 aagcgagatg gcacttttct tgtccgggag agcagtaaac agggctgcta tgcctgctct 3361 gtagtggtgg acggcgaagt aaagcattgt gtcataaaca aaacagcaac tggctatggc 3421 tttgccgagc cctataactt gtacagctct ctgaaagaac tggtgctaca ttaccaacac 3481 acctcccttg tgcagcacaa cgactccctc aatgtcacac tagcctaccc agtatatgca 3541 cagcagaggc gatgaggatc caccggatct agataactga tcataatcag ccataccaca 3601 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 3661 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 3721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 3781 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 3841 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3901 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3961 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 4021 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 4081 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 4141 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 4201 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 4261 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 4381 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 4441 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4501 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4561 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4621 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4681 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4741 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 4801 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4861 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4921 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 4981 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 5041 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 5101 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 5161 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 5221 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 5281 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 5341 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5401 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5461 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5521 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5581 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5641 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5701 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 5761 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 5821 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 5881 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 5941 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 6001 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 6061 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 6121 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 6181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 6241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 6301 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 6361 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 6421 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 6481 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 6541 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 6601 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 6661 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6721 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6781 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6841 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat //