LOCUS       V022336                 6471 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022336
VERSION     V022336
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1393..3126
                     /label="PIGT(NM_015937)"
                     /note="PIGT(NM_015937)"
                     /gene="PIGT"
     polyA_signal    3259..3380
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3387..3842)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3869..3973
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3975..4332
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4367..5161
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5393..5440
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5769..6357
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttcgaattc tgatggcggc ggctatgccg cttgctctgc tcgtcctgtt gctcctgggg
     1441 cccggcggct ggtgccttgc agaaccccca cgcgacagcc tgcgggagga acttgtcatc
     1501 accccgctgc cttccgggga cgtagccgcc acattccagt tccgcacgcg ctgggattcg
     1561 gagcttcagc gggaaggagt gtcccattac aggctctttc ccaaagccct ggggcagctg
     1621 atctccaagt attctctacg ggagctgcac ctgtcattca cacaaggctt ttggaggacc
     1681 cgatactggg ggccaccctt cctgcaggcc ccatcaggtg cagagctgtg ggtctggttc
     1741 caagacactg tcactgatgt ggataaatct tggaaggagc tcagtaatgt cctctcaggg
     1801 atcttctgcg cctctctcaa cttcatcgac tccaccaaca cagtcactcc cactgcctcc
     1861 ttcaaacccc tgggtctggc caatgacact gaccactact ttctgcgcta tgctgtgctg
     1921 ccgcgggagg tggtctgcac cgaaaacctc accccctgga agaagctctt gccctgtagt
     1981 tccaaggcag gcctctctgt gctgctgaag gcagatcgct tgttccacac cagctaccac
     2041 tcccaggcag tgcatatccg ccctgtttgc agaaatgcac gctgtactag catctcctgg
     2101 gagctgaggc agaccctgtc agttgtattt gatgccttca tcacggggca gggaaagaaa
     2161 gactggtccc tcttccggat gttctcccga accctcacgg agccctgccc cctggcttca
     2221 gagagccgag tctatgtgga catcaccacc tacaaccagg acaacgagac attagaggtg
     2281 cacccacccc cgaccactac atatcaggac gtcatcctag gcactcggaa gacctatgcc
     2341 atctatgact tgcttgacac cgccatgatc aacaactctc gaaacctcaa catccagctc
     2401 aagtggaaga gacccccaga gaatgaggcc cccccagtgc ccttcctgca tgcccagcgg
     2461 tacgtgagtg gctatgggct gcagaagggg gagctgagca cactgctgta caacacccac
     2521 ccataccggg ccttcccggt gctgctgctg gacaccgtac cctggtatct gcggctgtat
     2581 gtgcacaccc tcaccatcac ctccaagggc aaggagaaca aaccaagtta catccactac
     2641 cagcctgccc aggaccggct gcaaccccac ctcctggaga tgctgattca gctgccggcc
     2701 aactcagtca ccaaggtttc catccagttt gagcgggcgc tgctgaagtg gaccgagtac
     2761 acgccagatc ctaaccatgg cttctatgtc agcccatctg tcctcagcgc ccttgtgccc
     2821 agcatggtag cagccaagcc agtggactgg gaagagagtc ccctcttcaa cagcctgttc
     2881 ccagtctctg atggctctaa ctactttgtg cggctctaca gggagccgct gctggtgaac
     2941 ctgccgacac cggacttcag catgccctac aacgtgatct gcctcacgtg cactgtggtg
     3001 gccgtgtgct atggctcctt ctacaatctc ctcacccgaa ccttccacat cgaggagccc
     3061 cgcacaggtg gcctggccaa gcggctggcc aaccttatcc ggcgcgcccg aggtgtcccc
     3121 ccactctgag gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     3181 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3241 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3361 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     3421 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3481 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3541 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3601 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3661 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3721 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3781 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3841 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3901 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3961 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     4021 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4081 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4141 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4201 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4261 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4321 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4381 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4441 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4501 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4561 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4621 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4681 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4741 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4801 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4861 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4921 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4981 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5041 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5101 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5161 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5221 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5281 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     5341 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     5401 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5461 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5521 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5581 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5641 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5701 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5761 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5821 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5881 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5941 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6001 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6121 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6181 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6241 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6301 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6361 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6421 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
