LOCUS       V026590                 6643 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026590
VERSION     V026590
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..3267
                     /label="PIAS3(NM_006099)"
                     /note="PIAS3(NM_006099)"
                     /gene="PIAS3"
     polyA_signal    3431..3552
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3559..4014)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4041..4145
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4147..4504
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4539..5333
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5565..5612
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5941..6529
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatggcgg agctgggcga attaaagcac atggtgatga gtttccgggt gtctgagctc
     1441 caggtgcttc ttggctttgc tggccggaac aagagtggac ggaagcacga gctcctggcc
     1501 aaggctctgc acctcctgaa gtccagctgt gcccctagtg tccagatgaa gatcaaagag
     1561 ctttaccgac gacgctttcc ccggaagacc ctggggccct ctgatctctc ccttctctct
     1621 ttgccccctg gcacctctcc tgtaggctcc cctggtcctc tagctcccat tcccccaacg
     1681 ctgttggccc ctggcaccct gctgggcccc aagcgtgagg tggacatgca cccccctctg
     1741 ccccagcctg tgcaccctga tgtcaccatg aaaccattgc ccttctatga agtctatggg
     1801 gagctcatcc ggcccaccac ccttgcatcc acttctagcc agcggtttga ggaagcgcac
     1861 tttacctttg ccctcacacc ccagcaagtg cagcagattc ttacatccag agaggttctg
     1921 ccaggagcca aatgtgatta taccatacag gtgcagctaa ggttctgtct ctgtgagacc
     1981 agctgccccc aggaagatta ttttcccccc aacctctttg tcaaggtcaa tgggaaactg
     2041 tgccccctgc cgggttacct tcccccaacc aagaatgggg ccgagcccaa gaggcccagc
     2101 cgccccatca acatcacacc cctggctcga ctctcagcca ctgttcccaa caccattgtg
     2161 gtcaattggt catctgagtt cggacggaat tactccttgt ctgtgtacct ggtgaggcag
     2221 ttgactgcag gaacccttct acaaaaactc agagcaaagg gtatccggaa cccagaccac
     2281 tcgcgggcac tgatcaagga gaaattgact gctgaccctg acagtgaggt ggccactaca
     2341 agtctccggg tgtcactcat gtgcccgcta gggaagatgc gcctgactgt cccttgtcgt
     2401 gccctcacct gcgcccacct gcagagcttc gatgctgccc tttatctaca gatgaatgag
     2461 aagaagccta catggacatg tcctgtgtgt gacaagaagg ctccctatga atctcttatc
     2521 attgatggtt tatttatgga gattcttagt tcctgttcag attgtgatga gatccaattc
     2581 atggaagatg gatcctggtg cccaatgaaa cccaagaagg aggcatctga ggtttgcccc
     2641 ccgccagggt atgggctgga tggcctccag tacagcccag tccagggggg agatccatca
     2701 gagaataaga agaaggtcga agttattgac ttgacaatag aaagctcatc agatgaggag
     2761 gatctgcccc ctaccaagaa gcactgttct gtcacctcag ctgccatccc ggccctacct
     2821 ggaagcaaag gagtcctgac atctggccac cagccatcct cggtgctaag gagccctgct
     2881 atgggcacgt tgggtgggga tttcctgtcc agtctcccac tacatgagta cccacctgcc
     2941 ttcccactgg gagccgacat ccaaggttta gatttatttt catttcttca gacagagagt
     3001 cagcactatg gcccctctgt catcacctca ctagatgaac aggatgccct tggccacttc
     3061 ttccagtacc gagggacccc ttctcacttt ctgggcccac tggcccccac gctggggagc
     3121 tcccactgca gcgccactcc ggcgccccct cctggccgtg tcagcagcat tgtggcccct
     3181 gggggggcct tgagggaggg gcatggagga cccctgccct caggtccctc tttgactggc
     3241 tgtcggtcag acatcatttc cctggactga gaattctgca gtcgacggta ccgcgggccc
     3301 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
     3361 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3421 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3541 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     3601 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3661 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3721 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3781 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3841 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3901 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     3961 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     4021 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4141 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4201 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4261 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4321 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4381 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4441 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4501 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4561 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4621 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4681 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     4741 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4801 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4861 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4921 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4981 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5041 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     5101 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5161 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5221 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5281 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5341 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5401 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5461 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     5521 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     5581 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     5641 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     5701 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     5761 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     5821 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5881 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5941 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6001 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6061 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6121 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6181 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6241 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6301 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6361 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6421 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6481 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6541 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6601 gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
