LOCUS       V026589                 6712 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026589
VERSION     V026589
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..3336
                     /label="PIAS1(NM_016166)"
                     /note="PIAS1(NM_016166)"
                     /gene="PIAS1"
     polyA_signal    3500..3621
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3628..4083)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4110..4214
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4216..4573
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4608..5402
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5634..5681
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6010..6598
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatggcgg acagtgcgga actaaagcaa atggttatga gccttagagt ttctgaactc
     1441 caagtactgt tgggctacgc cgggagaaac aagcacggac gcaaacacga acttctcaca
     1501 aaagccctgc atttgctaaa ggctggctgt agtcctgctg tgcaaatgaa aattaaggaa
     1561 ctctataggc ggcggttccc acagaaaatc atgacgcctg cagacttgtc catccccaac
     1621 gtacattcaa gtcctatgcc agcaactttg tctccatcta ccattccaca actcacttac
     1681 gatggtcacc ctgcatcatc gccattactc cctgtttctc ttctgggacc taaacatgaa
     1741 ctggaactcc cacatcttac atcagctctt cacccagtcc atccggatat aaaacttcaa
     1801 aaattaccat tttatgattt actggatgaa ctgataaaac ccaccagtct agcatcagac
     1861 aacagtcagc gctttcgaga aacctgtttt gcatttgcct tgacaccaca acaagtgcag
     1921 caaatcagta gttccatgga tatttctggg accaaatgtg acttcacagt acaggtccag
     1981 ttaaggtttt gtttatcaga aaccagttgt ccacaagaag atcacttccc acccaatctt
     2041 tgtgtgaaag tgaatacaaa accttgcagc cttccaggtt accttccacc tacaaaaaat
     2101 ggcgtggaac caaagcgacc cagccgacca attaatatca cctcacttgt ccgactgtcc
     2161 acaacagtac caaacacgat tgttgtttct tggactgcag aaattggaag aaactattcc
     2221 atggcagtat atcttgtaaa acagttgtcc tcaacagttc ttcttcagag gttacgagca
     2281 aagggaataa ggaatccgga tcattctaga gctttaatta aagagaagtt gactgcggat
     2341 ccagacagtg aaatagctac aaccagccta agggtttctc tactatgtcc acttggtaaa
     2401 atgcggctga caattccgtg tcgggccctt acatgttctc atctacaatg ttttgacgca
     2461 actctttaca ttcagatgaa tgagaaaaaa ccaacctggg tttgtcctgt ctgtgataag
     2521 aaggctccat atgaacacct tattattgat ggcttgttta tggaaatcct aaagtactgt
     2581 acagactgtg atgaaataca atttaaggag gatggcactt gggcaccgat gagatcaaaa
     2641 aaggaagtac aggaagtttc tgcctcttac aatggagtcg atggatgctt gagctccaca
     2701 ttggagcatc aggtagcgtc tcaccaccag tcctcaaata aaaacaagaa agtagaagtg
     2761 attgacctaa ccatagacag ttcatctgat gaagaggaag aagagccatc tgccaagagg
     2821 acctgtcctt ccctatctcc cacatcacca ctaaataata aaggcatttt aagtcttcca
     2881 catcaagcat ctccagtatc ccgcacccca agccttcctg ctgtagacac aagctacatt
     2941 aatacctccc tcatccaaga ctataggcat cctttccaca tgacacccat gccttacgac
     3001 ttacaaggat tagatttctt tcctttctta tcaggagaca atcagcatta caacacctcc
     3061 ttgcttgccg ctgcagcagc agcagtttca gatgatcaag acctcctaca ctcgtctcgg
     3121 tttttcccgt atacctcctc acagatgttt cttgatcagt taagtgcagg aggcagtact
     3181 tctctgccaa ccaccaatgg aagcagtagt ggcagtaaca gcagcctggt ttcttccaac
     3241 agcctaaggg aaagccatag ccacaccgtc acaaacagga gcagcacgga cacggcatcc
     3301 atctttggca tcataccaga cattatttca ttggactgag aattctgcag tcgacggtac
     3361 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
     3421 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3481 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3541 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3601 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     3661 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3721 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3781 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3841 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3901 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3961 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     4021 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     4081 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4141 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4201 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     4261 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4321 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4381 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4441 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4501 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4561 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     4621 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4681 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     4741 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     4801 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4861 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4921 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4981 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5041 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5101 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     5161 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5221 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5281 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5341 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5401 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5461 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5521 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     5581 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     5641 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     5701 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     5761 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     5821 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     5881 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5941 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6001 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6061 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6121 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
