LOCUS       V035890                 6213 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035890
VERSION     V035890
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        join(5956..6213,1..100)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             135..929
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    1161..1208
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      1537..2125
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     enhancer        2300..2603
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2604..2807
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2852..3538
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent
                     protein with fast and complete maturation (Gadella et al.,
                     2023)"
                     /transl_table=1
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
                     /label="mScarlet3"
     CDS             3584..5107
                     /label="P4HB(NM_000918)"
                     /note="P4HB(NM_000918)"
                     /gene="P4HB"
     polyA_signal    5240..5361
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5368..5823)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5850..5954
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
       61 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
      121 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
      181 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
      241 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
      301 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
      361 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
      421 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
      481 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
      541 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
      601 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
      661 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
      721 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
      781 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
      841 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
      901 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
      961 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     1021 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1081 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     1141 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     1201 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     1261 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     1321 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     1381 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1441 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1501 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1561 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1621 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1681 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1741 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1801 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1861 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1921 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1981 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2041 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2101 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2161 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2221 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
     2281 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2341 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2401 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2461 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2521 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2581 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2641 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2701 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2761 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     2821 gtcagatccg ctagcgctac cggtcgccac catggatagc accgaggcag tgatcaagga
     2881 gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac ggccacgagt tcgagatcga
     2941 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga gggtgaccaa
     3001 gggtggcccc ctgcccttct cctgggacat cctgtcccct cagttcatgt acggctccag
     3061 ggccttcacg aagcaccccg ccgacatccc cgactactgg aagcagtcct tccccgaggg
     3121 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gccgtgtccg tggcccagga
     3181 cacctccctg gaggacggca ccctgatcta caaggtgaag ctccgcggca ccaacttccc
     3241 tcctgacggc cccgtaatgc agaagaagac aatgggctgg gaagcatcca ccgagcggtt
     3301 gtaccccgag gacgtcgtgc tgaagggcga cattaagatg gccctgcgcc tgaaggacgg
     3361 cggccgctac ctggcggact tcaagaccac ctacagggcc aagaagcccg tgcagatgcc
     3421 cggcgccttc aacatcgacc gcaagttgga catcacatcc cacaacgagg actacaccgt
     3481 ggtggaacag tacgaacgct ccgtggcccg ccactccacc ggcggctccg gtggctcctt
     3541 gtacaagggc ggcggaggga gtggaggagg gggaagcctc gagatgctgc gccgcgctct
     3601 gctgtgcctg gccgtggccg ccctggtgcg cgccgacgcc cccgaggagg aggaccacgt
     3661 cctggtgctg cggaaaagca acttcgcgga ggcgctggcg gcccacaagt acctgctggt
     3721 ggagttctat gccccttggt gtggccactg caaggctctg gcccctgagt atgccaaagc
     3781 cgctgggaag ctgaaggcag aaggttccga gatcaggttg gccaaggtgg acgccacgga
     3841 ggagtctgac ctggcccagc agtacggcgt gcgcggctat cccaccatca agttcttcag
     3901 gaatggagac acggcttccc ccaaggaata tacagctggc agagaggctg atgacatcgt
     3961 gaactggctg aagaagcgca cgggcccggc tgccaccacc ctgcctgacg gcgcagctgc
     4021 agagtccttg gtggagtcca gcgaggtggc tgtcatcggc ttcttcaagg acgtggagtc
     4081 ggactctgcc aagcagtttt tgcaggcagc agaggccatc gatgacatac catttgggat
     4141 cacttccaac agtgacgtgt tctccaaata ccagctcgac aaagatgggg ttgtcctctt
     4201 taagaagttt gatgaaggcc ggaacaactt tgaaggggag gtcaccaagg agaacctgct
     4261 ggactttatc aaacacaacc agctgcccct tgtcatcgag ttcaccgagc agacagcccc
     4321 gaagattttt ggaggtgaaa tcaagactca catcctgctg ttcttgccca agagtgtgtc
     4381 tgactatgac ggcaaactga gcaacttcaa aacagcagcc gagagcttca agggcaagat
     4441 cctgttcatc ttcatcgaca gcgaccacac cgacaaccag cgcatcctcg agttctttgg
     4501 cctgaagaag gaagagtgcc cggccgtgcg cctcatcacc ctggaggagg agatgaccaa
     4561 gtacaagccc gaatcggagg agctgacggc agagaggatc acagagttct gccaccgctt
     4621 cctggagggc aaaatcaagc cccacctgat gagccaggag ctgccggagg actgggacaa
     4681 gcagcctgtc aaggtgcttg ttgggaagaa ctttgaagac gtggcttttg atgagaaaaa
     4741 aaacgtcttt gtggagttct atgccccatg gtgtggtcac tgcaaacagt tggctcccat
     4801 ttgggataaa ctgggagaga cgtacaagga ccatgagaac atcgtcatcg ccaagatgga
     4861 ctcgactgcc aacgaggtgg aggccgtcaa agtgcacagc ttccccacac tcaagttctt
     4921 tcctgccagt gccgacagga cggtcattga ttacaacggg gaacgcacgc tggatggttt
     4981 taagaaattc ctggagagcg gtggccagga tggggcaggg gatgatgacg atctcgagga
     5041 cctggaagaa gcagaggagc cagacatgga ggaagacgat gatcagaaag ctgtgaaaga
     5101 tgaactgtaa ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
     5161 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     5221 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     5281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5341 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     5401 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5461 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5521 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5581 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     5641 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     5701 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     5761 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     5821 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5881 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5941 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     6001 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6061 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6121 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6181 gcccagttcc gcccattctc cgccccatgg ctg
//
