LOCUS       V021859                 6115 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021859
VERSION     V021859
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             624..2027
                     /label="Oprm1(NM_001038601)"
                     /note="Oprm1(NM_001038601)"
                     /gene="Oprm1"
     CDS             2061..2780
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2903..3024
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3031..3486)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3513..3617
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3619..3976
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4011..4805
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5037..5084
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5413..6001
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatggaca gcagcaccgg cccagggaac accagcgact
      661 gctcagaccc cttagctcag gcaagttgct ccccagcacc tggctcctgg ctcaacttgt
      721 cccacgttga tggcaaccag tccgatccat gcggtctgaa ccgcaccggg cttggcggga
      781 acgacagcct gtgccctcag accggcagcc cttccatggt cacagccatt accatcatgg
      841 ccctctactc tatcgtgtgt gtagtgggcc tcttcggaaa cttcctggtc atgtatgtga
      901 ttgtaagata caccaaaatg aagactgcca ccaacatcta cattttcaac cttgctctgg
      961 cagacgcctt agcgaccagt acactgccct ttcagagtgt caactacctg atgggaacat
     1021 ggcccttcgg aaccatcctc tgcaagatcg tgatctcaat agattactac aacatgttca
     1081 ccagcatatt caccctctgc accatgagcg tggaccgcta cattgctgtc tgccacccag
     1141 tcaaagccct ggatttccgt accccccgaa atgccaaaat cgtcaacgtc tgcaactgga
     1201 tcctctcttc tgccatcggt ctgcctgtaa tgttcatggc aaccacaaaa tacaggcagg
     1261 ggtccataga ttgcaccctc acgttctccc acccaacctg gtactgggag aacctgctca
     1321 aaatctgtgt ctttatcttc gctttcatca tgccggtcct catcatcact gtgtgttacg
     1381 gcctgatgat cttacgactc aagagcgttc gcatgctatc gggctccaaa gaaaaggaca
     1441 ggaatctgcg caggatcacc cggatggtgc tggtggtcgt ggctgtattt atcgtctgct
     1501 ggacccccat ccacatctac gtcatcatca aagcgctgat cacgattcca gaaaccacat
     1561 ttcagaccgt ttcctggcac ttctgcattg ctttgggtta cacgaacagc tgcctgaatc
     1621 cagttcttta cgccttcctg gatgaaaact tcaagcgatg cttcagagag ttctgcatcc
     1681 caacctcgtc cacgatcgaa cagcaaaact ccactcgagt ccgtcagaac actagggaac
     1741 atccctccac ggctaataca gtggatcgaa ctaaccacca gccagccctg gcagtcagcg
     1801 tggcccagat ctttacagga tatccttctc cgactcatgg tgaaaaaccc tgcaagagtt
     1861 acagggacag acctagaccc tgtggaagaa cgtggtcttt gaaatcgcgt gcagaatcca
     1921 atgtggagca cttccattgt ggagccgcat taatctataa caatgaacta aaaatagggc
     1981 cagtgtcctg gctccagatg cctgcgcacg tgctcgtgcg cccctgggga ggagggggaa
     2041 gcgatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2101 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2161 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2221 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     2281 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2341 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2401 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2461 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2521 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2581 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2641 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2701 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2761 tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt
     2821 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     2881 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2941 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3001 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     3061 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3121 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3181 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3241 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3301 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3361 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3421 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3481 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3541 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3601 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     3661 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3721 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3781 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3841 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3901 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3961 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4021 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4081 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4141 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     4201 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4261 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4321 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4381 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4441 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4501 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     4561 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4621 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4681 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4741 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4801 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     4861 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     4921 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     4981 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     5041 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     5101 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     5161 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     5221 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     5281 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5341 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5401 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5461 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5521 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     5581 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5641 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5701 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5761 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5821 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5881 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5941 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6001 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6061 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
