LOCUS       V034794                 5717 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034794
VERSION     V034794
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..1626
                     /label="OSTM1(NM_014028)"
                     /note="OSTM1(NM_014028)"
                     /gene="OSTM1"
     CDS             1663..2382
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKL
                     TLKFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNG
                     IKVNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMV
                     LLEFRTAAGITLGMDELYK*"
                     /label="EBFP2"
     polyA_signal    2505..2626
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2633..3088)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3115..3219
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3221..3578
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3613..4407
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4639..4686
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5015..5603
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatggag ccgggcccga cagccgcgca gcggaggtgt
      661 tcgttgccgc cgtggctgcc gctggggctg ctgctgtggt cggggctggc cctgggcgcg
      721 ctccccttcg gcagcagtcc gcacagggtc ttccacgacc tcctgtcgga gcagcagttg
      781 ctggaggtgg aggacttgtc cctgtccctc ctgcagggtg gagggctggg gcctctgtcg
      841 ctgcccccgg acctgccgga tctggatcct gagtgccggg agctcctgct ggacttcgcc
      901 aacagcagcg cagagctgac agggtgtctg gtgcgcagcg cccggcccgt gcgcctctgt
      961 cagacctgct accccctctt ccaacaggtc gtcagcaaga tggacaacat cagccgagcc
     1021 gcggggaata cttcagagag tcagagttgt gccagaagtc tcttaatggc agatagaatg
     1081 caaatagttg tgattctctc agaatttttt aataccacat ggcaggaggc aaattgtgca
     1141 aattgtttaa caaacaacag tgaagaatta tcaaacagca cagtatattt ccttaatcta
     1201 tttaatcaca ccctgacctg ctttgaacat aaccttcagg ggaatgcaca tagtctttta
     1261 cagacaaaaa attattcaga agtatgcaaa aactgccgtg aagcatacaa aactctgagt
     1321 agtctgtaca gtgaaatgca aaaaatgaat gaacttgaga ataaggctga acctggaaca
     1381 catttatgca ttgatgtgga agatgcaatg aacatcactc gaaaactatg gagtcgaact
     1441 ttcaactgtt cagtcccttg cagtgacaca gtgcctgtaa ttgctgtttc tgtgttcatt
     1501 ctctttctac ctgttgtctt ctaccttagt agctttcttc actcagagca aaagaaacgc
     1561 aaactcattc tgcccaaacg tctcaagtcc agtaccagtt ttgcaaatat tcaggaaaat
     1621 tcaaacggat ccggcggcgg agggagtgga ggagggggaa gcatggtgag caagggcgag
     1681 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1741 aagttcagcg tgaggggcga gggcgagggc gatgccacca acggcaagct gaccctgaag
     1801 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgagc
     1861 cacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     1921 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcacc
     1981 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2041 aagggcgtcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttc
     2101 aacagccaca acatctatat catggccgtc aagcagaaga acggcatcaa ggtgaacttc
     2161 aagatccgcc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2221 acccccatcg gcgacggccc cgtgctgctg cccgacagcc actacctgag cacccagtcc
     2281 gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttccgcacc
     2341 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     2401 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     2461 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     2521 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2581 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta
     2641 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     2701 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     2761 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     2821 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     2881 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2941 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     3001 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     3061 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3121 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3181 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     3241 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     3301 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3421 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3481 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3541 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     3601 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     3661 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     3721 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     3781 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     3841 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     3901 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     3961 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4021 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4081 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4141 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4201 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4261 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4321 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4381 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     4441 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4501 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4561 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     4621 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     4681 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     4741 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     4801 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     4861 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4921 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5161 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5701 gtattaccgc catgcat
//
