LOCUS       V036754                 6046 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036754
VERSION     V036754
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..1893
                     /label="NR2F1(NM_005654)"
                     /note="NR2F1(NM_005654)"
                     /gene="NR2F1"
     CDS             1909..1929
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             1930..1950
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             1951..1971
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             1993..2712
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2834..2955
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2962..3417)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3444..3548
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3550..3907
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3942..4736
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4968..5015
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5344..5932
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatggca atggtagtta gcagctggcg agatccgcag
      661 gacgacgtgg ccgggggcaa ccccggcggc cccaaccccg cagcgcaggc ggcccgcggc
      721 ggcggcggcg gcgccggcga gcagcagcag caggcgggct cgggcgcgcc gcacacgccg
      781 cagaccccgg gccagcccgg agcgcccgcc acccccggca cggcggggga caagggccag
      841 ggcccgcccg gttcgggcca gagccagcag cacatcgagt gcgtggtgtg cggggacaag
      901 tcgagcggca agcactacgg ccaattcacc tgcgagggct gcaaaagttt cttcaagagg
      961 agcgtccgca ggaacttaac ttacacatgc cgtgccaaca ggaactgtcc catcgaccag
     1021 caccaccgca accagtgcca atactgccgc ctcaagaagt gcctcaaagt gggcatgagg
     1081 cgggaagcgg ttcagcgagg aagaatgcct ccaacccagc ccaatccagg ccagtacgca
     1141 ctcaccaacg gggaccccct caacggccac tgctacctgt ccggctacat ctcgctgctg
     1201 ctgcgcgccg agccctaccc cacgtcgcgc tacggcagcc agtgcatgca gcccaacaac
     1261 attatgggca tcgagaacat ctgcgagctg gccgcgcgcc tgctcttcag cgccgtcgag
     1321 tgggcccgca acatcccctt cttcccggat ctgcagatca ccgaccaggt gtccctgcta
     1381 cgcctcacct ggagcgagct gttcgtgctc aacgcggccc agtgctctat gccgctgcac
     1441 gtggcgccgt tgctggccgc cgccggcctg catgcctcgc ccatgtctgc cgaccgcgtc
     1501 gtggccttca tggaccacat ccgcatcttc caggagcagg tggagaagct caaggcgcta
     1561 cacgtcgact cagccgagta cagctgcctc aaagccatcg tgctgttcac gtcagacgcc
     1621 tgtggcctgt cggatgcggc ccacatcgag agcctgcagg agaagtcgca gtgcgcactg
     1681 gaggagtacg tgaggagcca gtaccccaac cagcccagcc gttttggcaa actgctgctg
     1741 cgactgccct cgctgcgcac cgtgtcctcc tccgtcatcg agcagctctt cttcgtccgt
     1801 ttggtaggta aaacccccat cgaaactctc atccgcgata tgttactgtc tgggagcagc
     1861 ttcaactggc cttacatgtc catccagtgc tccgaattcg ccaccatgcc aaaaaagaag
     1921 agaaaggtac caaaaaagaa gagaaaggta ccaaaaaaga agagaaaggt acgggatcca
     1981 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     2041 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     2101 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     2161 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     2221 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2281 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2341 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2401 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2461 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2521 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2581 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2641 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2701 ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     2761 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     2821 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2881 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2941 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3001 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3061 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     3121 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3181 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3241 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3301 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3361 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     3421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3541 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     3601 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3661 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3721 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3781 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3841 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3901 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     3961 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4021 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4081 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     4141 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4201 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4261 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4321 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4381 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4441 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     4501 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4561 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4621 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4681 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     4741 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     4801 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     4861 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     4921 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     4981 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     5041 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     5101 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     5161 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     5221 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5281 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5341 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5401 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5461 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5521 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5581 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5641 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5701 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5761 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5821 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5881 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5941 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6001 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
