LOCUS       V034556                 6539 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034556
VERSION     V034556
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2455
                     /label="NFE2L2(NM_006164)"
                     /note="NFE2L2(NM_006164)"
                     /gene="NFE2L2"
     CDS             2495..3205
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    3327..3448
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3455..3910)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3937..4041
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4043..4400
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4435..5229
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5461..5508
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5837..6425
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgatggact tggagctgcc
      661 gccgccggga ctcccgtccc agcaggacat ggatttgatt gacatacttt ggaggcaaga
      721 tatagatctt ggagtaagtc gagaagtatt tgacttcagt cagcgacgga aagagtatga
      781 gctggaaaaa cagaaaaaac ttgaaaagga aagacaagaa caactccaaa aggagcaaga
      841 gaaagccttt ttcgctcagt tacaactaga tgaagagaca ggtgaatttc tcccaattca
      901 gccagcccag cacatccagt cagaaaccag tggatctgcc aactactccc aggttgccca
      961 cattcccaaa tcagatgctt tgtactttga tgactgcatg cagcttttgg cgcagacatt
     1021 cccgtttgta gatgacaatg aggtttcttc ggctacgttt cagtcacttg ttcctgatat
     1081 tcccggtcac atcgagagcc cagtcttcat tgctactaat caggctcagt cacctgaaac
     1141 ttctgttgct caggtagccc ctgttgattt agacggtatg caacaggaca ttgagcaagt
     1201 ttgggaggag ctattatcca ttcctgagtt acagtgtctt aatattgaaa atgacaagct
     1261 ggttgagact accatggttc caagtccaga agccaaactg acagaagttg acaattatca
     1321 tttttactca tctataccct caatggaaaa agaagtaggt aactgtagtc cacattttct
     1381 taatgctttt gaggattcct tcagcagcat cctctccaca gaagacccca accagttgac
     1441 agtgaactca ttaaattcag atgccacagt caacacagat tttggtgatg aattttattc
     1501 tgctttcata gctgagccca gtatcagcaa cagcatgccc tcacctgcta ctttaagcca
     1561 ttcactctct gaacttctaa atgggcccat tgatgtttct gatctatcac tttgcaaagc
     1621 tttcaaccaa aaccaccctg aaagcacagc agaattcaat gattctgact ccggcatttc
     1681 actaaacaca agtcccagtg tggcatcacc agaacactca gtggaatctt ccagctatgg
     1741 agacacacta cttggcctca gtgattctga agtggaagag ctagatagtg cccctggaag
     1801 tgtcaaacag aatggtccta aaacaccagt acattcttct ggggatatgg tacaaccctt
     1861 gtcaccatct caggggcaga gcactcacgt gcatgatgcc caatgtgaga acacaccaga
     1921 gaaagaattg cctgtaagtc ctggtcatcg gaaaacccca ttcacaaaag acaaacattc
     1981 aagccgcttg gaggctcatc tcacaagaga tgaacttagg gcaaaagctc tccatatccc
     2041 attccctgta gaaaaaatca ttaacctccc tgttgttgac ttcaacgaaa tgatgtccaa
     2101 agagcagttc aatgaagctc aacttgcatt aattcgggat atacgtagga ggggtaagaa
     2161 taaagtggct gctcagaatt gcagaaaaag aaaactggaa aatatagtag aactagagca
     2221 agatttagat catttgaaag atgaaaaaga aaaattgctc aaagaaaaag gagaaaatga
     2281 caaaagcctt cacctactga aaaaacaact cagcacctta tatctcgaag ttttcagcat
     2341 gctacgtgat gaagatggaa aaccttattc tcctagtgaa tactccctgc agcaaacaag
     2401 agatggcaat gttttccttg ttcccaaaag taagaagcca gatgttaaga aaaacggtgg
     2461 aggcggatca ggaggcggtg gatctgggga tcctatggtg agcaagggcg aggaggataa
     2521 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     2581 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     2641 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     2701 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     2761 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     2821 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     2881 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     2941 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     3001 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     3061 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     3121 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     3181 cggcatggac gagctgtaca agtaggcggc cgcgactcta gatcataatc agccatacca
     3241 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3301 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3361 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3421 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     3481 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3541 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3601 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3661 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3721 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3781 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3841 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3901 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3961 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4021 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     4081 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4141 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4201 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4261 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4321 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4381 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4441 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4501 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4561 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     4621 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4681 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4741 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4801 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4861 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4921 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     4981 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5041 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5101 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5161 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5221 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5281 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5341 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5401 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     5461 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     5521 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     5581 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     5641 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     5701 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5761 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6241 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6481 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
