LOCUS V022262 5463 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022262 VERSION V022262 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1390..2118 /label="N4BP2L1(NM_052818)" /note="N4BP2L1(NM_052818)" /gene="N4BP2L1" polyA_signal 2251..2372 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2379..2834) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2861..2965 /gene="bla" /label="AmpR promoter" promoter 2967..3324 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3359..4153 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4385..4432 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4761..5349 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag 1381 cttcgaatta tggaggacag tttccttcaa tcttttggga ggctgagcct ccagccccag 1441 cagcagcagc agcggcagcg gccgccccgg ccgcccccgc gggggacacc tcctcgccgc 1501 cacagcttta ggaaacacct ctacctcctg cgaggcctcc cgggctccgg gaaaactaca 1561 ctggccagac aattgcagca tgactttccc agggccctga ttttcagcac ggatgatttt 1621 ttcttcaggg aagatggtgc ctatgagttc aatcctgact tcctggagga agctcatgaa 1681 tggaaccaaa aaagagcaag aaaagcaatg aggaatggca tatcccccat tattattgat 1741 aataccaacc tccacgcctg ggaaatgaag ccctatgcag tcatggcact tgaaaataac 1801 tatgaagtta tattccgaga acctgacact cgctggaaat tcaacgttca agagttagca 1861 agaagaaaca ttcatggtgt ctcaagagaa aaaatccacc gaatgaaaga acggtatgaa 1921 cacgatgtta cttttcacag tgtgcttcat gcagaaaagc caagcagaat gaacagaaac 1981 caggacagga ataatgcatt gccttccaac aatgccagat actggaattc ctacacagag 2041 tttccaaacc ggagggccca cggtggattt acaaatgaga gctcctatca cagaaggggc 2101 ggttgtcacc atggatatta gggatccacc ggatctagat aactgatcat aatcagccat 2161 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 2221 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 2281 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 2341 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 2401 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 2461 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 2521 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 2581 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 2641 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 2701 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 2761 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 2821 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 2881 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 2941 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 3001 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3061 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3241 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 3301 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 3361 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 3421 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 3481 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 3541 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 3601 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 3661 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 3721 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 3781 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 3841 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 3901 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 3961 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4021 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4081 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4141 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 4201 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 4261 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 4321 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 4381 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 4441 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 4501 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 4561 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 4621 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 4681 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 4741 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 4801 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 4861 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 4921 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 4981 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5041 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5101 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5161 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5221 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 5281 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 5341 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 5401 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 5461 cat //