LOCUS       V043221                 6925 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043221
VERSION     V043221
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1378..3531
                     /label="Mpo(NM_010824)"
                     /note="Mpo(NM_010824)"
                     /gene="Mpo"
     CDS             3532..3549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     polyA_signal    3713..3834
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3841..4296)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4323..4427
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4429..4786
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4821..5615
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5847..5894
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6223..6811
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggt ggaggcggat caggaggcgg tggatctatg
     1381 aagctactct tggccttggc aggcctcctg gcccctctgg ccatgcttca gacctccaat
     1441 ggtgccaccc cagctctcct gggggaagtg gagaattcag ttgtgctgag ctgtatggaa
     1501 gaggccaagc agctagtgga cagagcctac aaggagcgga gagaaagcat caagcggagc
     1561 ctccaaagcg gctctgccag ccccacggag ctcctgtttt acttcaagca gccggtggcg
     1621 ggcaccagaa cagctgtgag ggccgctgat tatctacatg tggccctaga cctgctgaag
     1681 aggaagctgc agcccctgtg gccaaggcct ttcaatgtta cagatgtgtt gacacctgct
     1741 cagctgaatc tgttgtccgt gtcaagtggc tgtgcctatc aggacgtgag ggtgacatgc
     1801 ccaccgaatg acaagtatcg caccatcact ggacactgca acaacagacg aagccccact
     1861 ctgggggcct ccaaccgtgc ctttgtacgc tggttgcctg cagagtatga agatggcgtc
     1921 tccatgccct ttggctggac gcctggagtc aatcgcaatg gcttcaaggt gcccctggct
     1981 cgccaggtct ccaatgccat cgtgcgcttc cccaacgatc agctgaccaa ggaccaggag
     2041 cgtgcactca tgttcatgca gtggggacag tttctggatc atgatatcac cttgactcca
     2101 gagccagcta cccggttctc cttcttcact ggcctcaact gcgagaccag ctgcctgcag
     2161 cagccaccct gcttccccct caagatccca cccaatgacc ctcgaatcaa gaaccaaaag
     2221 gactgcatcc ccttcttccg ctcctgcccg gcatgcacca ggaacaacat caccattcgc
     2281 aaccagatca acgcgctcac ttccttcgtg gacgccagcg gggtgtacgg cagcgaggac
     2341 cccctagcca gaaagctgcg caacctcacc aaccagctgg ggctgctggc tatcaataca
     2401 cgcttccaag acaatggcag ggccctgatg ccctttgaca gcctgcacga tgacccctgc
     2461 ctcctcacca accgctccgc ccgcattcct tgttttctgg caggggacat gcgctccagc
     2521 gagatgccgg agctcacctc catgcacacc ctctttgttc gagagcataa ccggctggcc
     2581 acacagctca agcgcctgaa tcctcgatgg aatggggaga agctctacca ggaggcccgg
     2641 aagattgtag gggccatggt ccagatcatc acataccggg actacctgcc cttggtgttg
     2701 gggccagcag ccatgaagaa gtacctaccc cagtaccgat cttacaacga ctcagtagac
     2761 cctcgaatcg ccaatgtctt caccaacgct ttccgttatg gccacaccct catccaaccc
     2821 ttcatgttcc gcctgaacaa tcagtaccgg cccacagggc ccaacccccg agtcccactc
     2881 agcaaggtct tttttgccag ctggagagtc gtgttggaag gtggcattga ccccatcctc
     2941 cgaggcctca tggccactcc agccaaactg aatcgccaga atcaaattgt ggtggatgag
     3001 atccgggagc gactatttga gcaagtcatg aggataggac tggatttgcc tgctcttaac
     3061 atgcagcgca gccgggatca cggcctccca ggatacaatg cctggagacg cttttgtggg
     3121 cttccacagc ccagcacagt gggtgagctc ggcacggtgc tgaagaacct ggagttggca
     3181 cggaagctga tggcacaata tggcacgccc aacaacattg acatctggat gggcggtgtg
     3241 tccgagcccc tggagcccaa tggccgtgta ggccagctcc ttgcctgcct cattggcact
     3301 cagtttagga agctacgtga tggtgatcgg ttttggtggg agaacccagg cgtgttcagt
     3361 aaacagcaga gacaggcctt ggccagcatc tccttgcccc gtatcatctg tgacaacact
     3421 ggcatcacca ccgtgtcgaa gaacaacatc ttcatgtcca acacataccc ccgagacttt
     3481 gtcagctgta acacccttcc taaactgaac ctgacttcct ggaaggagac ccatcaccat
     3541 caccatcact aggaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag
     3601 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3661 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
     3841 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3901 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3961 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     4021 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     4081 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     4141 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     4201 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     4261 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     4321 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4381 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     4441 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4501 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4561 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4621 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4681 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4741 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     4801 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4861 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4921 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4981 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     5041 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5101 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5161 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5221 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     5281 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     5341 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     5401 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5461 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5521 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5581 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5641 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5701 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5761 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     5821 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     5881 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     5941 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     6001 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     6061 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6121 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6361 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6841 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6901 ggataaccgt attaccgcca tgcat
//
