LOCUS       V025156                 9608 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025156
VERSION     V025156
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..4810
                     /label="MET(NM_000245)"
                     /note="MET(NM_000245)"
                     /gene="MET"
     CDS             4844..6274
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK*"
                     /label="tdTomato"
     polyA_signal    6396..6517
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(6524..6979)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7006..7110
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        7112..7469
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7504..8298
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8530..8577
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8906..9494
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgaaggccc ccgctgtgct
      661 tgcacctggc atcctcgtgc tcctgtttac cttggtgcag aggagcaatg gggagtgtaa
      721 agaggcacta gcaaagtccg agatgaatgt gaatatgaag tatcagcttc ccaacttcac
      781 cgcggaaaca cccatccaga atgtcattct acatgagcat cacattttcc ttggtgccac
      841 taactacatt tatgttttaa atgaggaaga ccttcagaag gttgctgagt acaagactgg
      901 gcctgtgctg gaacacccag attgtttccc atgtcaggac tgcagcagca aagccaattt
      961 atcaggaggt gtttggaaag ataacatcaa catggctcta gttgtcgaca cctactatga
     1021 tgatcaactc attagctgtg gcagcgtcaa cagagggacc tgccagcgac atgtctttcc
     1081 ccacaatcat actgctgaca tacagtcgga ggttcactgc atattctccc cacagataga
     1141 agagcccagc cagtgtcctg actgtgtggt gagcgccctg ggagccaaag tcctttcatc
     1201 tgtaaaggac cggttcatca acttctttgt aggcaatacc ataaattctt cttatttccc
     1261 agatcatcca ttgcattcga tatcagtgag aaggctaaag gaaacgaaag atggttttat
     1321 gtttttgacg gaccagtcct acattgatgt tttacctgag ttcagagatt cttaccccat
     1381 taagtatgtc catgcctttg aaagcaacaa ttttatttac ttcttgacgg tccaaaggga
     1441 aactctagat gctcagactt ttcacacaag aataatcagg ttctgttcca taaactctgg
     1501 attgcattcc tacatggaaa tgcctctgga gtgtattctc acagaaaaga gaaaaaagag
     1561 atccacaaag aaggaagtgt ttaatatact tcaggctgcg tatgtcagca agcctggggc
     1621 ccagcttgct agacaaatag gagccagcct gaatgatgac attcttttcg gggtgttcgc
     1681 acaaagcaag ccagattctg ccgaaccaat ggatcgatct gccatgtgtg cattccctat
     1741 caaatatgtc aacgacttct tcaacaagat cgtcaacaaa aacaatgtga gatgtctcca
     1801 gcatttttac ggacccaatc atgagcactg ctttaatagg acacttctga gaaattcatc
     1861 aggctgtgaa gcgcgccgtg atgaatatcg aacagagttt accacagctt tgcagcgcgt
     1921 tgacttattc atgggtcaat tcagcgaagt cctcttaaca tctatatcca ccttcattaa
     1981 aggagacctc accatagcta atcttgggac atcagagggt cgcttcatgc aggttgtggt
     2041 ttctcgatca ggaccatcaa cccctcatgt gaattttctc ctggactccc atccagtgtc
     2101 tccagaagtg attgtggagc atacattaaa ccaaaatggc tacacactgg ttatcactgg
     2161 gaagaagatc acgaagatcc cattgaatgg cttgggctgc agacatttcc agtcctgcag
     2221 tcaatgcctc tctgccccac cctttgttca gtgtggctgg tgccacgaca aatgtgtgcg
     2281 atcggaggaa tgcctgagcg ggacatggac tcaacagatc tgtctgcctg caatctacaa
     2341 ggttttccca aatagtgcac cccttgaagg agggacaagg ctgaccatat gtggctggga
     2401 ctttggattt cggaggaata ataaatttga tttaaagaaa actagagttc tccttggaaa
     2461 tgagagctgc accttgactt taagtgagag cacgatgaat acattgaaat gcacagttgg
     2521 tcctgccatg aataagcatt tcaatatgtc cataattatt tcaaatggcc acgggacaac
     2581 acaatacagt acattctcct atgtggatcc tgtaataaca agtatttcgc cgaaatacgg
     2641 tcctatggct ggtggcactt tacttacttt aactggaaat tacctaaaca gtgggaattc
     2701 tagacacatt tcaattggtg gaaaaacatg tactttaaaa agtgtgtcaa acagtattct
     2761 tgaatgttat accccagccc aaaccatttc aactgagttt gctgttaaat tgaaaattga
     2821 cttagccaac cgagagacaa gcatcttcag ttaccgtgaa gatcccattg tctatgaaat
     2881 tcatccaacc aaatctttta ttagtggtgg gagcacaata acaggtgttg ggaaaaacct
     2941 gaattcagtt agtgtcccga gaatggtcat aaatgtgcat gaagcaggaa ggaactttac
     3001 agtggcatgt caacatcgct ctaattcaga gataatctgt tgtaccactc cttccctgca
     3061 acagctgaat ctgcaactcc ccctgaaaac caaagccttt ttcatgttag atgggatcct
     3121 ttccaaatac tttgatctca tttatgtaca taatcctgtg tttaagcctt ttgaaaagcc
     3181 agtgatgatc tcaatgggca atgaaaatgt actggaaatt aagggaaatg atattgaccc
     3241 tgaagcagtt aaaggtgaag tgttaaaagt tggaaataag agctgtgaga atatacactt
     3301 acattctgaa gccgttttat gcacggtccc caatgacctg ctgaaattga acagcgagct
     3361 aaatatagag tggaagcaag caatttcttc aaccgtcctt ggaaaagtaa tagttcaacc
     3421 agatcagaat ttcacaggat tgattgctgg tgttgtctca atatcaacag cactgttatt
     3481 actacttggg tttttcctgt ggctgaaaaa gagaaagcaa attaaagatc tgggcagtga
     3541 attagttcgc tacgatgcaa gagtacacac tcctcatttg gataggcttg taagtgcccg
     3601 aagtgtaagc ccaactacag aaatggtttc aaatgaatct gtagactacc gagctacttt
     3661 tccagaagat cagtttccta attcatctca gaacggttca tgccgacaag tgcagtatcc
     3721 tctgacagac atgtccccca tcctaactag tggggactct gatatatcca gtccattact
     3781 gcaaaatact gtccacattg acctcagtgc tctaaatcca gagctggtcc aggcagtgca
     3841 gcatgtagtg attgggccca gtagcctgat tgtgcatttc aatgaagtca taggaagagg
     3901 gcattttggt tgtgtatatc atgggacttt gttggacaat gatggcaaga aaattcactg
     3961 tgctgtgaaa tccttgaaca gaatcactga cataggagaa gtttcccaat ttctgaccga
     4021 gggaatcatc atgaaagatt ttagtcatcc caatgtcctc tcgctcctgg gaatctgcct
     4081 gcgaagtgaa gggtctccgc tggtggtcct accatacatg aaacatggag atcttcgaaa
     4141 tttcattcga aatgagactc ataatccaac tgtaaaagat cttattggct ttggtcttca
     4201 agtagccaaa ggcatgaaat atcttgcaag caaaaagttt gtccacagag acttggctgc
     4261 aagaaactgt atgctggatg aaaaattcac agtcaaggtt gctgattttg gtcttgccag
     4321 agacatgtat gataaagaat actatagtgt acacaacaaa acaggtgcaa agctgccagt
     4381 gaagtggatg gctttggaaa gtctgcaaac tcaaaagttt accaccaagt cagatgtgtg
     4441 gtcctttggc gtgctcctct gggagctgat gacaagagga gccccacctt atcctgacgt
     4501 aaacaccttt gatataactg tttacttgtt gcaagggaga agactcctac aacccgaata
     4561 ctgcccagac cccttatatg aagtaatgct aaaatgctgg caccctaaag ccgaaatgcg
     4621 cccatccttt tctgaactgg tgtcccggat atcagcgatc ttctctactt tcattgggga
     4681 gcactatgtc catgtgaacg ctacttatgt gaacgtaaaa tgtgtcgctc cgtatccttc
     4741 tctgttgtca tcagaagata acgctgatga tgaggtggac acacgaccag cctccttctg
     4801 ggagacatca ggaggagggg gaagcgatcc accggtcgcc accatggtga gcaagggcga
     4861 ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     4921 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4981 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     5041 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     5101 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     5161 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     5221 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     5281 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     5341 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     5401 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     5461 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     5521 ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa
     5581 caacatggcc gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
     5641 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     5701 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     5761 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
     5821 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     5881 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
     5941 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     6001 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
     6061 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
     6121 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc
     6181 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
     6241 gttcctgtac ggcatggacg agctgtacaa gtaggcggcc gcgactctag atcataatca
     6301 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     6361 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     6421 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6481 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     6541 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     6601 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     6661 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     6721 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     6781 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     6841 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     6901 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     6961 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7021 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7081 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     7141 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7201 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7261 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7381 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7441 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     7501 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     7561 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     7621 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     7681 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     7741 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     7801 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     7861 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     7921 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     7981 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     8041 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     8101 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     8161 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     8221 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     8281 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     8341 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8401 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8461 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     8521 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     8581 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     8641 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     8701 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     8761 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8881 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8941 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9001 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9061 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9121 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9181 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9241 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9301 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9361 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9421 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9481 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9541 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9601 ccatgcat
//
