LOCUS       V020906                 7933 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020906
VERSION     V020906
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        1..304
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        305..508
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             565..3768
                     /label="Luzp1(NM_024452)"
                     /note="Luzp1(NM_024452)"
                     /gene="Luzp1"
     CDS             3820..4539
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced GFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="mEGFP"
     polyA_signal    4661..4782
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4789..5244)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5271..5375
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5377..5734
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5769..6563
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6795..6842
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7171..7759
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgaggc caccatggca gaactcacta actacaagga tgctgcctcc
      601 aaccgccacc tgcgatttaa gttacagagc ctgagccgcc gcctcgatga gttagaggaa
      661 gctacaaaaa acctccagag agcggaggat gagctcctgg acctccagga caaggtgatc
      721 caggcagagg gcagcgactc cagcacgctg gctgagatcg aggtgctgcg ccagcgggta
      781 ctgaagattg aaggtaaaga tgaggaaatt aagagagcag aggatctatg tcatacgatg
      841 aaggagaaac ttgaagagga agaaaacctt acgcgagagc taaaatctga aattgagcgg
      901 cttcagaaac gaatggttga tttagagaag ctagaggagg ctttgagcag gagtaagaat
      961 gaatgttccc agctttgttt gagcctcaat gaagagagaa atctgaccaa gaaaatctct
     1021 tctgagttgg aaatgctcag agtcaaagtg aaagagctag aatcttcaga ggaccgctta
     1081 gataaaactg agcagagttt agtgtcagaa ttagaaaagc taaaatcatt aaccctgagc
     1141 tttgtaaatg agagaaaata cttgaatgaa aaggagaaag aaaatgagaa aataataaaa
     1201 gagcttactc aaaaactgga gcagaacaag aaaatgaacc gagaccacat gagaaatgct
     1261 tccacttttc tggaaaggaa tgacctacgg atagaggacg gcatttcctc tacactgtca
     1321 tccaaagagt caaaaaggaa gggcagtcta gactacctaa agcaggtcga gaatgaaacc
     1381 agagacaaat cagaaaatga aaagaaccgg aatcaggaag acaacaaagt gaaagacctt
     1441 aaccaagaga ttgagaaact taagacacaa atcaaacatt ttgaatcttt ggaagaggag
     1501 cttaagaaaa tgagggctaa aaacaatgac cttcaggata attacctaac tgagctgaat
     1561 agaaacagaa gcttagccag ccagctagag gagataaagc tgcaggtcag gaaacaaaaa
     1621 gagttaggaa atggagacat agaaggtgaa gatgctttcc ttttgggcag aggcaggcac
     1681 gagaggacta agctcaaagg ccatgggagt gaggcctctg tgtccaagca tacatcacgg
     1741 gaactgtctc cccagcataa gcgggaaagg ctccggaaca gggagtttgc tctcagtaac
     1801 gaacactatt ctctaagcag caaacaggct tcttctccag ttttcacaaa caaaagggca
     1861 gcaaaagcct ccaacatggg aatgggtaca gacagtggga ctcaggagac aaagagaact
     1921 gaagaccgat ttgcacctgg gtcctctcac agtgaaggga aaaggggtag ggagcagcca
     1981 tcagtgctta gccgttaccc ccctgctgcc caggagcaca ctaaagtttg gaaaggagct
     2041 cccaagccag gcactgagag tggacttaag ggaaaagtag agaagacaac acgaacattt
     2101 agtgattcca ctcatgtatc tgtgcccaat gatatagtgg ggaaaggtga taagacttct
     2161 gacctctcct ctgaggcaca ctgtgggaag agaggacagg tgcctggcca tgcaagtcag
     2221 ggaacacagg ctgtagagtc tagctgctct aaggccatcg gagctctctc ctcatctcaa
     2281 aaagcctcct cagaaggact ttctaagggt aaaaagactg ccaatggcct agcggctgat
     2341 gccaattttt caaattctaa agctccaatt ttatcaaaat atccttatag ttcaagaagc
     2401 caagaaaata tacttcaggg cttttcattg ccaaataaag aaggagttga tcagcctgta
     2461 gcagttgtga tggaagacag cagtcagcat gaagccctga ggtgccgtgt catcaaatcc
     2521 agtggcagag agaagccaga ctcagatgat gacctggaca tagagtcttt tgttactgcc
     2581 aagttggtga acacaactat cactccagag ccagagccca aaccacagcc aaactcaaga
     2641 gaaaaggtta agtccagagg gggaactaga actgccctat ttgaaaatga taaaaatgct
     2701 gcaatagaaa atgactctgt gaaacctacc aggccttcct ccaatgctat agaattccca
     2761 gatgccaact gtgctggggt gaaaaatcaa aggcccttca gccctagaga ggccctgcgg
     2821 tctagagcaa ttataaaacc cgttatcatt gataaggatg tgaaaaaaat catgggtgga
     2881 tctggaactg aggttgtatt ggagaagcag aaatccacct ctaaatctgt gacaagcaaa
     2941 gtgacaagca gcattaccat ctacccctct gacagcagtg ggcctagagc cgtcccgagc
     3001 gaggccccaa gggagaggca tacatccacc agcaacatcc aggtggggcc cccagagctc
     3061 acagcaatta gcaaccatgt cagctcccct cttgagctct ctattcataa acatgacatc
     3121 accttgcagc tcacagaagc tgaaagagtg ggagatgggt ctccaaagaa tagagcagag
     3181 atggtggtct caagaagcag tatcctgata aaaccatcag agtctgtgga gaagaatagt
     3241 catgtgcccc cagccgagac aatcaggtgg aaaagccata gtgcctcttc agacagcaga
     3301 cacatcacag tgcggaatgc ctggaagagt aagagagact tgaaatgttc agaagacccc
     3361 ccgactggaa taggtaggaa catggaagct actaacgcct atacgcagag gccctgcaca
     3421 gacttcttag aacttgagca gcccaggtca cagccatctg aacagggcgc tcgaagagta
     3481 ggaaattcag gggatgctcc tgagctctcc ccgaggagga cccaaagtag cctcaccgca
     3541 tcagaggtgc tcacccgcag ggaccggatg ggaggtgcca tcactgctgc ctcctgcaac
     3601 cactcatcaa gcatggagga aggggaggac agcacatttg ttaccagcag gcgaatacac
     3661 aacccgctgg agcattctga actgcctgga aagcagggac tgccagagcc tgagccagtt
     3721 tggactgagg agcggctgca cccagcaaag ccttatgctg aggaagaccg aattctgcag
     3781 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
     3841 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3901 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3961 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     4021 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4081 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4141 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4201 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4261 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4321 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4381 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     4441 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4501 gccgggatca ctctcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     4561 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4621 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4681 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4741 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     4801 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4861 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4921 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4981 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     5041 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5101 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5161 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5221 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5281 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5341 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     5401 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5461 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5581 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5641 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5701 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     5761 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5821 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5881 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5941 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6001 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6061 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6121 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6181 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6241 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     6301 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6361 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6421 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6481 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6541 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6601 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6661 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6721 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     6781 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     6841 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     6901 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     6961 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     7021 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7081 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7861 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
     7921 atatggagtt ccg
//
