LOCUS       V020589                 5961 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020589
VERSION     V020589
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             626..1846
                     /codon_start=1
                     /gene=""
                     /note=""
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /protein_id=""
                     /transl_table=1
                     /translation="MAAPGARRPLLLLLLAGLAHS,APALFEVKDNNGTACIMASFSAS
                     FLTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNT
                     TRYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVT
                     IVLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNG
                     TCLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQF
                     GMNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKA
                     LALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRK
                     RSHAGYQTI"
                     /label="LAMP1"
     CDS             1907..2626
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     polyA_signal    2749..2870
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2877..3332)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3359..3463
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3465..3822
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3857..4651
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4883..4930
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5259..5847
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcg ccaccatggc ggccccgggc gcccggcggc cgctgctcct
      661 gttgctgctg gcaggccttg cacacagcgc cccagcactg ttcgaggtga aagacaacaa
      721 cggcacagcg tgtataatgg ccagcttctc tgcctccttt ctgaccacct atgaggctgg
      781 acatgtttct aaggtctcga atatgaccct gccagcctct gcagaagtcc tgaagaatag
      841 cagctcttgt ggtgaaaaga atgcttctga gcccaccctc gcaatcacct ttggagaagg
      901 atatttactg aaactcacct tcacaaaaaa cacaacacgt tacagtgtcc agcacatgta
      961 tttcacatat aacctgtcag acacacaatt ctttcccaat gccagctcca aagggcccga
     1021 cactgtggat tccacaactg acatcaaggc agacatcaac aaaacatacc gatgtgtcag
     1081 cgacatcagg gtctacatga agaatgtgac cattgtgctc tgggacgcta ctatccaggc
     1141 ctacctgccg agtagcaact tcagcaagga agagacacgc tgcccacagg atcaaccttc
     1201 cccaactact gggccaccca gcccctcacc accacttgtg cccacaaacc ccagtgtgtc
     1261 caagtacaat gtgactggtg acaatggaac ctgcctgctg gcctctatgg cactgcaact
     1321 caacatcacc tacatgaaga aggacaacac gactgtgacc agagcattca acatcaaccc
     1381 aagtgacaaa tatagtggga cttgcggtgc ccagttggtg accctgaagg tggggaacaa
     1441 gagcagagtc ctggagctgc agtttgggat gaatgccact tctagcctgt ttttcctgca
     1501 aggagttcag ttgaacatga ctcttcctga tgccatagag cccacgttca gcacctccaa
     1561 ctattccctg aaagctcttc aggccagtgt cggcaactca tacaagtgca acagtgagga
     1621 gcacatcttt gtcagcaagg cgctcgccct caatgtcttc agcgtgcaag tccaggcttt
     1681 cagggtagaa agtgacaggt ttgggtctgt ggaagagtgt gtacaggacg gtaacaacat
     1741 gctgatcccc attgctgtgg gcggggccct ggcagggctg gtcctcatcg tcctcatcgc
     1801 ctacctcatc ggcaggaaga ggagtcacgc gggctatcag accatctcgg aattcggctc
     1861 caccggctcc accggctcca ccggcgcgga tccaccggtc gcccatatgg tgagcaaggg
     1921 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1981 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2041 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     2101 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     2161 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2221 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2281 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2341 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2401 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2461 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     2521 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2581 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     2641 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2701 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2761 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2821 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     2881 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2941 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3001 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3061 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3121 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3181 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3241 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3301 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3361 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3421 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3481 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3541 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3601 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3661 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3721 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3781 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3841 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3901 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3961 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4021 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4081 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4141 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4201 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4261 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4321 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4381 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4441 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4501 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4561 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4621 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4681 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4741 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4801 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4861 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4921 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4981 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5041 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     5101 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5161 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5221 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5281 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5341 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5401 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5461 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5521 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5581 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5641 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5701 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5761 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5821 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5881 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5941 aaccgtatta ccgccatgca t
//
