LOCUS       V027221                 6313 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027221
VERSION     V027221
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced blue variant of GFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EBFP"
     CDS             1336..2937
                     /label="LPCAT1(NM_024830)"
                     /note="LPCAT1(NM_024830)"
                     /gene="LPCAT1"
     polyA_signal    3101..3222
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3229..3684)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3711..3815
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3817..4174
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4209..5003
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5235..5282
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5611..6199
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc cacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggaatgag gctgcgggga tgcggacccc gggccgcccc tgcctccagc
     1381 gcaggggcca gcgacgctcg gctgctggcg cccccggggc ggaacccctt cgtgcacgag
     1441 ctgcgcctca gcgccctgca gaaggcccag gtggccctca tgacactgac gctcttcccg
     1501 gtccggctcc tggttgccgc tgccatgatg ctgctggcct ggcccctcgc acttgtcgca
     1561 tccctgggct ctgcggagaa ggaacccgag cagcccccgg ccctgtggag gaaggttgtg
     1621 gacttcctgc tgaaggccat catgcgcacc atgtggttcg ccggcggctt ccaccgggtg
     1681 gccgtgaagg ggcggcaggc gctgcccacc gaggcggcca tcctcacgct cgcgcctcac
     1741 tcgtcctact tcgacgccat ccctgtgacc atgacgatgt cctccatcgt gatgaaggca
     1801 gagagcagag acatcccgat ctggggaact ctgatccagt atatacggcc tgtgttcgtg
     1861 tcccggtcag accaggattc tcgcaggaaa acagtagaag aaatcaagag acgggcgcag
     1921 tccaacggaa agtggccaca gataatgatt tttccagaag gaacttgtac aaacaggacc
     1981 tgcctaatta ccttcaaacc tggtgcattc atccctggag cgcccgtcca gcctgtggtt
     2041 ttacgatatc caaataaact ggacaccatc acatggacgt ggcaaggacc tggagcgctg
     2101 gaaatcctgt ggctcacgct gtgtcagttt cacaaccaag tggaaatcga gttccttcct
     2161 gtgtacagcc cttctgagga ggagaagagg aaccccgcgc tgtatgccag caacgtgcgg
     2221 cgagtcatgg ccgaggcctt gggtgtctcc gtgactgact acacgttcga ggactgccag
     2281 ctggccctgg cggaaggaca gctccgtctc cccgctgaca cttgcctttt agaatttgcc
     2341 aggctcgtgc ggggcctcgg gctaaaacca gaaaagcttg aaaaagatct ggacagatac
     2401 tcagaaagag ccaggatgaa gggaggagag aagataggta ttgcggagtt tgccgcctcc
     2461 ctggaagtcc ccgtttctga cttgctggaa gacatgtttt cactgttcga cgagagcggc
     2521 agcggcgagg tggacctgcg agagtgtgtg gttgccctgt ctgtcgtctg ccggccggcc
     2581 cggaccctgg acaccatcca gctggctttc aagatgtacg gagcgcaaga ggacggcagc
     2641 gtcggcgaag gtgacctgtc ctgcatcctc aagacggccc tgggggtggc agagctcacc
     2701 gtgaccgacc tattccgagc cattgaccaa gaggagaagg ggaagatcac attcgctgac
     2761 ttccacaggt ttgcagaaat gtaccctgcc ttcgcagagg aatacctgta cccggatcag
     2821 acacatttcg aaagctgtgc agagacctca cctgcgccaa tcccaaacgg cttctgtgcc
     2881 gatttcagcc cggaaaactc agacgctggg cggaagcctg ttcgcaagaa gctggattag
     2941 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
     3001 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3061 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3121 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3181 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     3241 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3301 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3361 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3421 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3481 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3541 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3601 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3661 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3721 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3781 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     3841 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3901 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3961 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4021 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4081 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4141 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     4201 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4261 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4321 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4381 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4441 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4501 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4561 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4621 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4681 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     4741 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4801 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4861 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4921 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4981 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5041 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5101 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5161 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5221 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     5281 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5341 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5401 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     5461 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5521 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5581 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5641 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5701 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5761 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5821 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5881 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5941 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6001 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6061 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6241 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6301 taccgccatg cat
//
