LOCUS       V021848                 6758 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021848
VERSION     V021848
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2652
                     /label="LIMD1(NM_014240)"
                     /note="LIMD1(NM_014240)"
                     /gene="LIMD1"
     CDS             2704..3423
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3546..3667
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3674..4129)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4156..4260
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4262..4619
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4654..5448
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5680..5727
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6056..6644
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatggat aagtatgacg acctgggcct ggaggccagt
      661 aaattcatcg aggacctgaa catgtatgag gcctctaagg atgggctctt ccgagtggac
      721 aagggtgcag gcaacaaccc cgagtttgag gaaactcgca gggtgttcgc caccaagatg
      781 gccaaaatcc acctccagca gcagcagcag cagctcctgc aggaggagac tctgcccagg
      841 gggagtagag gccctgtcaa tggagggggc cgcctgggcc cacaggcccg ttgggaagtt
      901 gtgggcagca agctgactgt ggatggtgct gccaagcctc ctcttgctgc ctcgacaggg
      961 gcacctgggg cagtcaccac cctcgctgct gggcagcccc cgtacccacc gcaggagcag
     1021 agatccaggc catacctgca tggcacgagg catggcagcc aggactgtgg ttccagggag
     1081 agcctggcga cttctgagat gtctgctttc caccagccag gcccctgtga ggatccttcc
     1141 tgcctcactc atggagacta ttatgacaac ctctccttgg caagcccaaa gtggggtgac
     1201 aaaccaggag tgtcccccag catcggcctg agtgtaggga gtgggtggcc tagctccccg
     1261 gggagtgacc caccactgcc caaaccctgc ggggaccatc ccctaaatca ccgacagctc
     1321 tccctgagct ccagcaggtc ttctgagggt agcctcggtg gtcagaatag tggcattggt
     1381 ggccgcagca gcgagaagcc aacaggcctt tggtccactg cctcctccca gcgggtgagc
     1441 cctggcctgc cttccccaaa cttggagaac ggagcaccag ctgtggggcc tgttcagccc
     1501 aggacccctt ctgtgtcagc acccttggcc ctgagctgcc ccaggcaagg aggtcttcca
     1561 agatcaaact cggggctggg gggtgaggtt tcaggtgtga tgtccaaacc caatgtggac
     1621 ccccaaccct ggttccagga tgggcccaaa tcttaccttt ccagttctgc cccgtcatcc
     1681 tcgccagctg gtctggacgg ttcacagcag ggtgcggtcc ctgggctggg gccgaagcct
     1741 ggctgcacag accttggcac tggtcccaag ctcagcccca ccagtcttgt ccatccagtg
     1801 atgtccaccc tgcctgagtt atcttgtaaa gagggtcccc tgggctggtc ttctgatggt
     1861 agcctgggat ctgtgctcct ggacagcccc agctccccta gggtaaggct gccctgccag
     1921 cccctcgtcc caggtcctga gctgagaccc tctgctgctg agttgaaatt agaagccctc
     1981 acccaacgtc tggagcgaga gatggatgct cacccgaagg ctgattactt tggagcctgt
     2041 gtgaaatgca gcaaaggggt gtttggggct ggccaggcct gtcaggccat ggggaacctc
     2101 taccatgaca catgcttcac ctgtgcagct tgcagccgga agctgagagg aaaagccttt
     2161 tattttgtca acggcaaagt gttttgtgaa gaagacttcc tgtactctgg tttccagcag
     2221 tcggctgaca ggtgttttct ttgtggacat ctgatcatgg acatgatcct gcaagccctg
     2281 gggaagtcct accaccccgg ctgtttccgc tgtgtcatct gtaatgagtg tttggatggg
     2341 gtgcccttca ccgtggactc agagaacaag atctactgtg tccgagatta ccacaaggtg
     2401 ctggccccca agtgtgcagc ctgtgggctt cccatccttc cacctgaggg ctcagatgag
     2461 accatccgtg tcgtgtccat ggacagagac taccacgtgg agtgttacca ctgcgaggac
     2521 tgtggtctgg agctcaatga tgaagatggc caccgctgtt atccgctgga ggaccacctg
     2581 ttctgtcact cctgccacgt gaagaggctg gagaagagac cctcatctac agcccttcac
     2641 cagcaccact tccgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc
     2701 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2761 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     2821 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     2881 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     2941 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3001 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3061 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3121 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3181 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3241 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3301 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     3361 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3421 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     3481 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3541 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3601 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3661 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3721 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3781 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3841 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3901 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3961 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     4021 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     4081 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     4141 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4201 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4261 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4321 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4381 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4441 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4501 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4561 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4621 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4681 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4741 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4801 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4861 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4921 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4981 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5041 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5101 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5161 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     5221 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5281 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5341 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5401 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5461 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5521 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5581 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     5641 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     5701 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     5761 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5821 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5881 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6181 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6721 cccctgattc tgtggataac cgtattaccg ccatgcat
//
