LOCUS       V020168                 6768 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020168
VERSION     V020168
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1354..3423
                     /label="LATS1(NM_001270519)"
                     /note="LATS1(NM_001270519)"
                     /gene="LATS1"
     polyA_signal    3556..3677
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3684..4139)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4166..4270
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4272..4629
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4664..5458
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5690..5737
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6066..6654
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagg aggaggggga agcatgaaga ggagtgaaaa gccagaagga
     1381 tatagacaaa tgaggcctaa gacctttcct gccagtaact atactgtcag tagccggcaa
     1441 atgttacaag aaattcggga atcccttagg aatttatcta aaccatctga tgctgctaag
     1501 gctgagcata acatgagtaa aatgtcaacc gaagatcctc gacaagtcag aaatccaccc
     1561 aaatttggga cgcatcataa agccttgcag gaaattcgaa actctctgct tccatttgca
     1621 aatgaaacaa attcttctcg gagtacttca gaagttaatc cacaaatgct tcaagacttg
     1681 caagctgctg gatttgatga ggatatggtt atacaagctc ttcagaaaac taacaacaga
     1741 agtatagaag cagcaattga attcattagt aaaatgagtt accaagatcc tcgacgagag
     1801 cagatggctg cagcagctgc cagacctatt aatgccagca tgaaaccagg gaatgtgcag
     1861 caatcagtta accgcaaaca gagctggaaa ggttctaaag aatccttagt tcctcagagg
     1921 catggcccgc cactaggaga aagtgtggcc tatcattctg agagtcccaa ctcacagaca
     1981 gatgtaggaa gacctttgtc tggatctggt atatcagcat ttgttcaagc tcaccctagc
     2041 aacggacaga gagtgaaccc cccaccacca cctcaagtaa ggagtgttac tcctccacca
     2101 cctccaagag gccagactcc ccctccaaga ggtacaactc cacctccccc ttcatgggaa
     2161 ccaaactctc aaacaaagcg ctattctgga aacatggaat acgtaatctc ccgaatctct
     2221 cctgtcccac ctggggcatg gcaagagggc tatcctccac cacctctcaa cacttccccc
     2281 atgaatcctc ctaatcaagg acagagaggc attagttctg ttcctgttgg cagacaacca
     2341 atcatcatgc agagttctag caaatttaac tttccatcag ggagacctgg aatgcagaat
     2401 ggtactggac aaactgattt catgatacac caaaatgttg tccctgctgg cactgtgaat
     2461 cggcagccac cacctccata tcctctgaca gcagctaatg gacaaagccc ttctgcttta
     2521 caaacagggg gatctgctgc tccttcgtca tatacaaatg gaagtattcc tcagtctatg
     2581 atggtgccaa acagaaatag tcataacatg gaactatata acattagtgt acctggactg
     2641 caaacaaatt ggcctcagtc atcttctgct ccagcccagt catccccgag cagtgggcat
     2701 gaaatcccta catggcaacc taacatacca gtgaggtcaa attcttttaa taacccatta
     2761 ggaaatagag caagtcactc tgctaattct cagccttctg ctacaacagt cactgcaatt
     2821 acaccagctc ctattcaaca gcctgtgaaa agtatgcgtg tattaaaacc agagctacag
     2881 actgctttag cacctacaca cccttcttgg ataccacagc caattcaaac tgttcaaccc
     2941 agtccttttc ctgagggaac cgcttcaaat gtgactgtga tgccacctgt tgctgaagct
     3001 ccaaactatc aaggaccacc accaccctac ccaaaacatc tgctgcacca aaacccatct
     3061 gttcctccat acgagtcaat cagtaagcct agcaaagagg atcagccaag cttgcccaag
     3121 gaagatgaga gtgaaaagag ttatgaaaat gttgatagtg gggataaaga aaagaaacag
     3181 attacaactt cacctattac tgttaggaaa aacaagaaag atgaagagcg aagggaatct
     3241 cgtattcaaa gttattctcc tcaagcattt aaattcttta tggagcaaca tgtagaaaat
     3301 gtactcaaat ctcatcagca gcgtctacat cgtaaaaaac aattagagaa tgaaatgatg
     3361 cgggtaaaac cttttaaaat gtccattttt atacttaatc atctgtttgc ttggtgttta
     3421 ttttaaggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     3481 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     3541 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3601 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3661 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     3721 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     3781 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     3841 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     3901 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     3961 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4021 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4081 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4141 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4201 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4261 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4321 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4381 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4441 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4501 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4561 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4621 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     4681 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     4741 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4801 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     4861 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4921 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     4981 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5041 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5101 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5161 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     5221 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5281 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5341 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5401 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5461 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     5521 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5581 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     5641 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     5701 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     5761 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     5821 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     5881 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     5941 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6001 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6061 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6121 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6181 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6241 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6301 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6361 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6421 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6481 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6541 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6601 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6661 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6721 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
