LOCUS       V040098                 5591 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040098
VERSION     V040098
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..1488
                     /label="LACTB2(NM_016027)"
                     /note="LACTB2(NM_016027)"
                     /gene="LACTB2"
     CDS             1537..2256
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2379..2500
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2507..2962)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        2989..3093
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3095..3452
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3487..4281
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4513..4560
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4889..5477
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatggct gctgtactgc agcgcgtcga gcggctgtcc
      661 aatcgagtcg tgcgtgtgtt gggctgtaac ccgggtccca tgaccctcca aggcaccaac
      721 acctacctag tggggaccgg ccccaggaga atcctcattg acactggaga accagcaatt
      781 ccagaataca tcagctgttt aaagcaggct ctaactgaat ttaacacagc aatccaggaa
      841 attgtagtga ctcactggca ccgagatcat tctggaggca taggagatat ttgtaaaagc
      901 atcaataatg acactaccta ttgcattaaa aaactcccac ggaatcctca gagagaagaa
      961 attataggaa atggagagca acaatatgtt tatctgaaag atggagatgt gattaagact
     1021 gagggagcca ctctaagagt tctatatacc cctggccaca ctgatgatca catggctcta
     1081 ctcttagaag aggaaaatgc tatcttttct ggagattgca tcctagggga aggaacaacg
     1141 gtatttgaag acctctatga ttatatgaac tctttaaaag agttattgaa aatcaaagct
     1201 gatattatat atccaggaca tggcccagta attcataatg ctgaagctaa aattcaacaa
     1261 tacatttctc acagaaatat tcgagagcag caaattctta cattatttcg tgagaacttt
     1321 gagaaatcat ttacagtaat ggagcttgta aaaattattt acaagaatac tcctgagaat
     1381 ttacatgaaa tggctaaaca taatctctta cttcatttga aaaaactaga aaaagaagga
     1441 aaaatattta gcaacacaga tcctgacaag aaatggaaag ctcatcttgg cggcggaggg
     1501 agtggaggag ggggaagcga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     1561 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1621 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1681 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1741 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1801 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1861 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1921 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1981 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2041 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2101 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     2161 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2221 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
     2281 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2341 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2401 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2461 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
     2521 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     2581 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     2641 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     2701 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     2761 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     2821 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     2881 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     2941 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3001 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3061 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     3121 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3181 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3241 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3301 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3361 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3421 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     3481 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3541 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3601 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3661 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3721 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3781 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3841 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     3901 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     3961 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     4021 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4081 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4141 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4201 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4261 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4321 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4381 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4441 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4501 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     4561 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     4621 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     4681 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     4741 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4801 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5521 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5581 ccgccatgca t
//
