LOCUS       V018915                 6957 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018915
VERSION     V018915
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1351..3591
                     /label="KIF3B(NM_004798)"
                     /note="KIF3B(NM_004798)"
                     /gene="KIF3B"
     polyA_signal    3745..3866
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3873..4328)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4355..4459
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4461..4818
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4853..5647
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5879..5926
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6255..6843
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct atgtcaaagt tgaaaagctc agagtcagtc
     1381 agggtggtgg ttcgctgtcg gcccatgaat ggcaaggaaa aggctgcttc gtatgacaaa
     1441 gtggtggatg tggatgttaa gctggggcag gtgtctgtga agaaccccaa agggacggcc
     1501 catgaaatgc ccaagacctt cacctttgat gccgtctatg actggaatgc caagcagttt
     1561 gaactgtacg atgagacgtt ccgaccactt gttgactctg tcctgcaagg tttcaatgga
     1621 accatttttg cctatggaca aactgggaca ggaaaaacct acaccatgga aggaatccgt
     1681 ggtgaccctg aaaaaagagg agtcattcct aactcatttg accatatctt cacccacatc
     1741 tctcgatccc agaatcaaca atacctggtc agggcttctt acttagagat ctaccaggag
     1801 gagatccgag atttgctctc aaaggatcag accaaaaggc ttgagctcaa agagaggcct
     1861 gacacaggag tgtatgtgaa agacctgtct tcctttgtca ccaagagtgt gaaggagata
     1921 gagcatgtga tgaatgtggg gaaccagaac cgttctgtcg gtgctaccaa catgaacgag
     1981 cacagctcgc gttctcatgc aattttcgtt atcactattg agtgcagcga ggtgggcctc
     2041 gatggtgaaa accacatccg tgtaggaaaa ttgaaccttg tagatcttgc tggcagcgaa
     2101 cggcaagcca agaccggcgc acaaggggag agattaaaag aagctaccaa gatcaacctc
     2161 tccctttccg ctttgggtaa tgtcatctct gctctagtgg acggcaaaag cactcacatt
     2221 ccatatcggg actcaaagct taccaggctc ctccaagatt cccttggtgg caatgccaag
     2281 actgtgatgg tggccaacgt ggggcctgcc tcttacaacg tagaagagac tctgaccact
     2341 ctgcgatatg ccaaccgtgc caaaaacatt aagaacaaac caagggtcaa tgaggacccc
     2401 aaggatgccc tccttcgaga attccaggaa gagattgctc ggctcaaggc ccagctggaa
     2461 aaacggtcca ttggtaggag gaagaggcga gagaagcgga gggaaggtgg tggcagtggt
     2521 gggggtgggg aagaggagga ggaggaggga gaagagggtg aggaggaagg ggatgataag
     2581 gatgattact ggcgggaaca gcaagaaaaa ctggagattg agaagcgggc cattgtagag
     2641 gatcacagct tggttgcaga ggagaagatg aggctgctga aggagaaaga gaaaaagatg
     2701 gaggacctgc ggcgggagaa ggatgctgcc gagatgctgg gcgccaagat caaggccatg
     2761 gagagtaagt tgcttgttgg aggaaaaaat atagtagatc atacgaatga acagcagaaa
     2821 atcctggagc agaaacgaca ggaaattgca gagcagaaac gtcgagaaag agaaatccag
     2881 caacagatgg aaagtcgaga tgaggagacc ttggaactta aagagacata cagctcattg
     2941 cagcaagagg tggacatcaa gaccaaaaaa ctcaaaaagc tcttctccaa gcttcaggca
     3001 gtgaaggctg agatccatga cctccaagaa gaacacatca aggagcgcca agagctagag
     3061 cagactcaga atgagctcac cagggagctg aaactcaagc atcttattat agaaaacttt
     3121 atccctctgg aagaaaaaag taaaattatg aatagagcct tctttgatga agaggaagat
     3181 cattggaaac tacatcctat aaccagactg gagaaccagc agatgatgaa gcggccagtc
     3241 tcagccgtgg gatataagag accattgagc cagcacgcaa gaatgtccat gatgattcgt
     3301 ccagaggccc gatatagggc agaaaacatt gtgctgttag agctggacat gcccagccgg
     3361 accaccagag actatgaggg tccagccatt gcccccaagg tccaggctgc attggatgcg
     3421 gctctgcagg atgaagatga gatacaggtg gatgcatcat catttgaaag cactgcaaat
     3481 aagaaatcca aggccaggcc taaaagtgga aggaagtcgg gatcctcctc ctcttcctca
     3541 ggaacccctg catctcagct ttatccacag tctcgggggc tggttccaaa gtaagtcgac
     3601 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
     3661 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3721 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3841 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     3901 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3961 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4021 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4081 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4141 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4201 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4261 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4321 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4441 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4501 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4561 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4621 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4741 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4801 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4861 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4921 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4981 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     5041 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5101 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5161 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5221 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5281 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5341 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     5401 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5461 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5521 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5581 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5641 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5701 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5761 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5821 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5881 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5941 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     6001 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     6061 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     6121 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6181 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6241 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6301 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6361 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6421 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6481 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6541 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6601 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6661 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6721 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6781 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6841 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6901 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
