LOCUS       V019661                 6573 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019661
VERSION     V019661
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2496
                     /label="KEAP1(NM_203500)"
                     /note="KEAP1(NM_203500)"
                     /gene="KEAP1"
     CDS             2530..3240
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    3361..3482
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3489..3944)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3971..4075
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4077..4434
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4469..5263
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5495..5542
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5871..6459
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgcag ccagatccca ggcctagcgg ggctggggcc
      661 tgctgccgat tcctgcccct gcagtcacag tgccctgagg gggcagggga cgcggtgatg
      721 tacgcctcca ctgagtgcaa ggcggaggtg acgccctccc agcatggcaa ccgcaccttc
      781 agctacaccc tggaggatca taccaagcag gcctttggca tcatgaacga gctgcggctc
      841 agccagcagc tgtgtgacgt cacactgcag gtcaagtacc aggatgcacc ggccgcccag
      901 ttcatggccc acaaggtggt gctggcctca tccagccctg tcttcaaggc catgttcacc
      961 aacgggctgc gggagcaggg catggaggtg gtgtccattg agggtatcca ccccaaggtc
     1021 atggagcgcc tcattgaatt cgcctacacg gcctccatct ccatgggcga gaagtgtgtc
     1081 ctccacgtca tgaacggtgc tgtcatgtac cagatcgaca gcgttgtccg tgcctgcagt
     1141 gacttcctgg tgcagcagct ggaccccagc aatgccatcg gcatcgccaa cttcgctgag
     1201 cagattggct gtgtggagtt gcaccagcgt gcccgggagt acatctacat gcattttggg
     1261 gaggtggcca agcaagagga gttcttcaac ctgtcccact gccaactggt gaccctcatc
     1321 agccgggacg acctgaacgt gcgctgcgag tccgaggtct tccacgcctg catcaactgg
     1381 gtcaagtacg actgcgaaca gcgacggttc tacgtccagg cgctgctgcg ggccgtgcgc
     1441 tgccactcgt tgacgccgaa cttcctgcag atgcagctgc agaagtgcga gatcctgcag
     1501 tccgactccc gctgcaagga ctacctggtc aagatcttcg aggagctcac cctgcacaag
     1561 cccacgcagg tgatgccctg ccgggcgccc aaggtgggcc gcctgatcta caccgcgggc
     1621 ggctacttcc gacagtcgct cagctacctg gaggcttaca accccagtga cggcacctgg
     1681 ctccggttgg cggacctgca ggtgccgcgg agcggcctgg ccggctgcgt ggtgggcggg
     1741 ctgttgtacg ccgtgggcgg caggaacaac tcgcccgacg gcaacaccga ctccagcgcc
     1801 ctggactgtt acaaccccat gaccaatcag tggtcgccct gcgcccccat gagcgtgccc
     1861 cgtaaccgca tcggggtggg ggtcatcgat ggccacatct atgccgtcgg cggctcccac
     1921 ggctgcatcc accacaacag tgtggagagg tatgagccag agcgggatga gtggcacttg
     1981 gtggccccaa tgctgacacg aaggatcggg gtgggcgtgg ctgtcctcaa tcgtctcctt
     2041 tatgccgtgg ggggctttga cgggacaaac cgccttaatt cagctgagtg ttactaccca
     2101 gagaggaacg agtggcgaat gatcacagca atgaacacca tccgaagcgg ggcaggcgtc
     2161 tgcgtcctgc acaactgtat ctatgctgct gggggctatg atggtcagga ccagctgaac
     2221 agcgtggagc gctacgatgt ggaaacagag acgtggactt tcgtagcccc catgaagcac
     2281 cggcgaagtg ccctggggat cactgtccac caggggagaa tctacgtcct tggaggctat
     2341 gatggtcaca cgttcctgga cagtgtggag tgttacgacc cagatacaga cacctggagc
     2401 gaggtgaccc gaatgacatc gggccggagt ggggtgggcg tggctgtcac catggagccc
     2461 tgccggaagc agattgacca gcagaactgt acctgtggag gagggggaag cgatccaccg
     2521 gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     2581 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     2641 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     2701 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     2761 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     2821 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2881 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2941 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     3001 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     3061 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     3121 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     3181 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag
     3241 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     3301 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     3361 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3421 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3481 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     3541 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     3601 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     3661 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     3721 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     3781 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     3841 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     3901 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     3961 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4021 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     4081 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4141 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4201 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4261 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4321 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4381 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     4441 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     4501 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     4561 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4621 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     4681 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     4741 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4801 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     4861 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     4921 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     4981 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     5041 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5101 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5161 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     5221 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     5281 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     5341 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5401 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     5461 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     5521 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     5581 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     5641 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     5701 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     5761 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5821 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5881 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5941 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6001 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6061 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6121 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6181 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6241 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6301 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6361 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6421 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6481 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6541 gattctgtgg ataaccgtat taccgccatg cat
//
