LOCUS       V037536                 7299 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037536
VERSION     V037536
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EYFP"
     CDS             1381..3954
                     /label="KCNB1(NM_004975)"
                     /note="KCNB1(NM_004975)"
                     /gene="KCNB1"
     polyA_signal    4087..4208
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4215..4670)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4697..4801
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4803..5160
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5195..5989
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6221..6268
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6597..7185
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggcggag ggagtggagg agggggaagc
     1381 atgccggcgg gcatgacgaa gcatggctcc cgctccacca gctcgctgcc gcccgagccc
     1441 atggagatcg tgcgcagcaa ggcgtgctct cggcgggtcc gcctcaacgt cggggggctg
     1501 gcgcacgagg tactctggcg taccctggac cgcctgcccc gcacgcggct gggcaagctc
     1561 cgcgactgca acacgcacga ctcgctgctc gaggtgtgcg atgactacag cctcgacgac
     1621 aacgagtact tctttgaccg ccacccgggc gccttcacct ccatcctcaa cttctaccgc
     1681 actgggcgac tgcacatgat ggaggagatg tgcgcgctca gcttcagcca agagctcgac
     1741 tactggggca tcgacgagat ctacctggag tcctgctgcc aggcccgcta ccaccagaag
     1801 aaagagcaga tgaacgagga gctcaagcgt gaggccgaga ccctacggga gcgggaaggc
     1861 gaggagttcg ataacacgtg ctgcgcagag aagaggaaaa aactctggga cctactggag
     1921 aagcccaatt cctctgtggc tgccaagatc cttgccataa tttccatcat gttcatcgtc
     1981 ctctccacca ttgccctgtc cctcaacacg ctgcctgagc tacagagcct cgatgagttc
     2041 ggccagtcca cagacaaccc ccagctggcc cacgtggagg ccgtgtgcat cgcatggttc
     2101 accatggagt acctgctgag gttcctctcc tcgcccaaga agtggaagtt cttcaagggc
     2161 ccactcaatg ccattgactt gttggccatt ctgccatact atgtcaccat tttcctcacc
     2221 gaatccaaca agagcgtgct gcaattccag aatgtccgcc gcgtggtcca gatcttccgc
     2281 atcatgcgaa ttctccgcat ccttaagctt gcacgccact ccactggcct ccagtctctg
     2341 ggcttcactt tgcggaggag ctacaatgag ttgggcttgc tcatcctctt ccttgccatg
     2401 ggcattatga tcttctccag ccttgtcttc tttgctgaga aggatgagga cgacaccaag
     2461 ttcaaaagca tcccagcctc tttctggtgg gccaccatca ccatgactac tgttgggtat
     2521 ggagacatct accccaagac tctcctgggg aaaattgttg ggggactctg ctgcattgca
     2581 ggagtcctgg tgattgctct tcccatcccc atcatcgtca ataacttctc tgagttctat
     2641 aaggagcaga agagacagga gaaagcaatc aaacggcgag aggctctgga gagagccaag
     2701 aggaatggca gcatcgtatc catgaacatg aaggatgctt ttgcccggag cattgagatg
     2761 atggacattg tggttgagaa aaatggggag aatatgggta agaaagacaa agtacaagat
     2821 aaccacttgt ctcctaacaa atggaaatgg acaaagagga cactgtctga aaccagctca
     2881 agtaagtcct ttgaaaccaa ggaacaggga tcccctgaaa aagccagatc gtcttctagt
     2941 cctcagcacc tgaacgttca gcagttggaa gacatgtaca ataagatggc caagacccaa
     3001 tcccaaccca tcctcaatac caaggagtca gcagcacaga gcaaaccaaa ggaagaactt
     3061 gaaatggaga gtatccccag ccccgtagcc cctctgccca ctcgcacaga aggggtcatt
     3121 gacatgcgaa gtatgtcaag cattgatagt ttcattagct gtgccacaga cttccctgag
     3181 gccaccagat tctcccacag ccctttgaca tcactcccca gcaagactgg gggcagcaca
     3241 gccccagaag tgggctggcg gggagctctg ggtgccagtg gtggtaggtt tgtggaggcc
     3301 aaccccagcc ctgatgccag ccagcactct agtttcttca tcgagagccc caagagttcc
     3361 atgaaaacta acaacccttt gaagctccga gcacttaaag tcaacttcat ggagggtgac
     3421 cccagtccac tcctccccgt tctagggatg taccatgacc ctctcaggaa ccgggggagt
     3481 gctgcggctg ctgtcgctgg actggagtgt gccacgcttt tggacaaggc tgtgctgagc
     3541 ccagagtcct ccatctacac cacagcaagt gctaagacac ccccccggtc tcctgagaaa
     3601 cacacagcaa tagcgttcaa ctttgaggcg ggtgtccacc agtacattga cgcagacaca
     3661 gatgatgagg gacagctgct ctacagtgtg gactccagcc cccccaaaag cctccctggg
     3721 agcaccagtc cgaagttcag cacggggaca agatcggaga aaaaccactt tgaaagctcc
     3781 cctttaccca cctcccctaa gttcttaagg cagaactgta tttactccac agaagcattg
     3841 actggaaaag gccccagtgg tcaggaaaag tgcaaacttg agaaccacat ctcccctgac
     3901 gtccgtgtgt tgccaggggg aggagcccat ggaagcacac gagatcagag catctgagga
     3961 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     4021 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     4081 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4141 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4201 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     4261 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4321 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4381 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     4441 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4501 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4561 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4621 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     4681 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4741 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4801 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4861 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4921 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4981 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5041 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5101 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5161 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5221 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5281 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5341 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     5401 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5461 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5521 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5581 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5641 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5701 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5761 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5821 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5881 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5941 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6001 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6061 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6121 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     6181 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     6241 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     6301 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     6361 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     6421 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     6481 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6541 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6601 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6661 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6721 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6781 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6841 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6901 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6961 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7021 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7081 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7141 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7201 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7261 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
