LOCUS       V026474                10177 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026474
VERSION     V026474
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             624..6089
                     /label="ITGB4(NM_000213)"
                     /note="ITGB4(NM_000213)"
                     /gene="ITGB4"
     CDS             6123..6842
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     polyA_signal    6965..7086
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(7093..7548)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7575..7679
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        7681..8038
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8073..8867
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9099..9146
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9475..10063
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatggcag ggccacgccc cagcccatgg gccaggctgc
      661 tcctggcagc cttgatcagc gtcagcctct ctgggacctt ggcaaaccgc tgcaagaagg
      721 ccccagtgaa gagctgcacg gagtgtgtcc gtgtggataa ggactgcgcc tactgcacag
      781 acgagatgtt cagggaccgg cgctgcaaca cccaggcgga gctgctggcc gcgggctgcc
      841 agcgggagag catcgtggtc atggagagca gcttccaaat cacagaggag acccagattg
      901 acaccaccct gcggcgcagc cagatgtccc cccaaggcct gcgggtccgt ctgcggcccg
      961 gtgaggagcg gcattttgag ctggaggtgt ttgagccact ggagagcccc gtggacctgt
     1021 acatcctcat ggacttctcc aactccatgt ccgatgatct ggacaacctc aagaagatgg
     1081 ggcagaacct ggctcgggtc ctgagccagc tcaccagcga ctacactatt ggatttggca
     1141 agtttgtgga caaagtcagc gtcccgcaga cggacatgag gcctgagaag ctgaaggagc
     1201 cctggcccaa cagtgacccc cccttctcct tcaagaacgt catcagcctg acagaagatg
     1261 tggatgagtt ccggaataaa ctgcagggag agcggatctc aggcaacctg gatgctcctg
     1321 agggcggctt cgatgccatc ctgcagacag ctgtgtgcac gagggacatt ggctggcgcc
     1381 cggacagcac ccacctgctg gtcttctcca ccgagtcagc cttccactat gaggctgatg
     1441 gcgccaacgt gctggctggc atcatgagcc gcaacgatga acggtgccac ctggacacca
     1501 cgggcaccta cacccagtac aggacacagg actacccgtc ggtgcccacc ctggtgcgcc
     1561 tgctcgccaa gcacaacatc atccccatct ttgctgtcac caactactcc tatagctact
     1621 acgagaagct tcacacctat ttccctgtct cctcactggg ggtgctgcag gaggactcgt
     1681 ccaacatcgt ggagctgctg gaggaggcct tcaatcggat ccgctccaac ctggacatcc
     1741 gggccctaga cagcccccga ggccttcgga cagaggtcac ctccaagatg ttccagaaga
     1801 cgaggactgg gtcctttcac atccggcggg gggaagtggg tatataccag gtgcagctgc
     1861 gggcccttga gcacgtggat gggacgcacg tgtgccagct gccggaggac cagaagggca
     1921 acatccatct gaaaccttcc ttctccgacg gcctcaagat ggacgcgggc atcatctgtg
     1981 atgtgtgcac ctgcgagctg caaaaagagg tgcggtcagc tcgctgcagc ttcaacggag
     2041 acttcgtgtg cggacagtgt gtgtgcagcg agggctggag tggccagacc tgcaactgct
     2101 ccaccggctc tctgagtgac attcagccct gcctgcggga gggcgaggac aagccgtgct
     2161 ccggccgtgg ggagtgccag tgcgggcact gtgtgtgcta cggcgaaggc cgctacgagg
     2221 gtcagttctg cgagtatgac aacttccagt gtccccgcac ttccgggttc ctctgcaatg
     2281 accgaggacg ctgctccatg ggccagtgtg tgtgtgagcc tggttggaca ggcccaagct
     2341 gtgactgtcc cctcagcaat gccacctgca tcgacagcaa tgggggcatc tgtaatggac
     2401 gtggccactg tgagtgtggc cgctgccact gccaccagca gtcgctctac acggacacca
     2461 tctgcgagat caactactcg gcgatccacc cgggcctctg cgaggaccta cgctcctgcg
     2521 tgcagtgcca ggcgtggggc accggcgaga agaaggggcg cacgtgtgag gaatgcaact
     2581 tcaaggtcaa gatggtggac gagcttaaga gagccgagga ggtggtggtg cgctgctcct
     2641 tccgggacga ggatgacgac tgcacctaca gctacaccat ggaaggtgac ggcgcccctg
     2701 ggcccaacag cactgtcctg gtgcacaaga agaaggactg ccctccgggc tccttctggt
     2761 ggctcatccc cctgctcctc ctcctcctgc cgctcctggc cctgctactg ctgctatgct
     2821 ggaagtactg tgcctgctgc aaggcctgcc tggcacttct cccgtgctgc aaccgaggtc
     2881 acatggtggg ctttaaggaa gaccactaca tgctgcggga gaacctgatg gcctctgacc
     2941 acttggacac gcccatgctg cgcagcggga acctcaaggg ccgtgacgtg gtccgctgga
     3001 aggtcaccaa caacatgcag cggcctggct ttgccactca tgccgccagc atcaacccca
     3061 cagagctggt gccctacggg ctgtccttgc gcctggcccg cctttgcacc gagaacctgc
     3121 tgaagcctga cactcgggag tgcgcccagc tgcgccagga ggtggaggag aacctgaacg
     3181 aggtctacag gcagatctcc ggtgtacaca agctccagca gaccaagttc cggcagcagc
     3241 ccaatgccgg gaaaaagcaa gaccacacca ttgtggacac agtgctgatg gcgccccgct
     3301 cggccaagcc ggccctgctg aagcttacag agaagcaggt ggaacagagg gccttccacg
     3361 acctcaaggt ggcccccggc tactacaccc tcactgcaga ccaggacgcc cggggcatgg
     3421 tggagttcca ggagggcgtg gagctggtgg acgtacgggt gcccctcttt atccggcctg
     3481 aggatgacga cgagaagcag ctgctggtgg aggccatcga cgtgcccgca ggcactgcca
     3541 ccctcggccg ccgcctggta aacatcacca tcatcaagga gcaagccaga gacgtggtgt
     3601 cctttgagca gcctgagttc tcggtcagcc gcggggacca ggtggcccgc atccctgtca
     3661 tccggcgtgt cctggacggc gggaagtccc aggtctccta ccgcacacag gatggcaccg
     3721 cgcagggcaa ccgggactac atccccgtgg agggtgagct gctgttccag cctggggagg
     3781 cctggaaaga gctgcaggtg aagctcctgg agctgcaaga agttgactcc ctcctgcggg
     3841 gccgccaggt ccgccgtttc cacgtccagc tcagcaaccc taagtttggg gcccacctgg
     3901 gccagcccca ctccaccacc atcatcatca gggacccaga tgaactggac cggagcttca
     3961 cgagtcagat gttgtcatca cagccacccc ctcacggcga cctgggcgcc ccgcagaacc
     4021 ccaatgctaa ggccgctggg tccaggaaga tccatttcaa ctggctgccc ccttctggca
     4081 agccaatggg gtacagggta aagtactgga ttcagggtga ctccgaatcc gaagcccacc
     4141 tgctcgacag caaggtgccc tcagtggagc tcaccaacct gtacccgtat tgcgactatg
     4201 agatgaaggt gtgcgcctac ggggctcagg gcgagggacc ctacagctcc ctggtgtcct
     4261 gccgcaccca ccaggaagtg cccagcgagc cagggcgtct ggccttcaat gtcgtctcct
     4321 ccacggtgac ccagctgagc tgggctgagc cggctgagac caacggtgag atcacagcct
     4381 acgaggtctg ctatggcctg gtcaacgatg acaaccgacc tattgggccc atgaagaaag
     4441 tgctggttga caaccctaag aaccggatgc tgcttattga gaaccttcgg gagtcccagc
     4501 cctaccgcta cacggtgaag gcgcgcaacg gggccggctg ggggcctgag cgggaggcca
     4561 tcatcaacct ggccacccag cccaagaggc ccatgtccat ccccatcatc cctgacatcc
     4621 ctatcgtgga cgcccagagc ggggaggact acgacagctt ccttatgtac agcgatgacg
     4681 ttctacgctc tccatcgggc agccagaggc ccagcgtctc cgatgacact ggctgcggct
     4741 ggaagttcga gcccctgctg ggggaggagc tggacctgcg gcgcgtcacg tggcggctgc
     4801 ccccggagct catcccgcgc ctgtcggcca gcagcgggcg ctcctccgac gccgaggcgc
     4861 cccacgggcc cccggacgac ggcggcgcgg gcgggaaggg cggcagcctg ccccgcagtg
     4921 cgacacccgg gccccccgga gagcacctgg tgaatggccg gatggacttt gccttcccgg
     4981 gcagcaccaa ctccctgcac aggatgacca cgaccagtgc tgctgcctat ggcacccacc
     5041 tgagcccaca cgtgccccac cgcgtgctaa gcacatcctc caccctcaca cgggactaca
     5101 actcactgac ccgctcagaa cactcacact cgaccacact gcccagggac tactccaccc
     5161 tcacctccgt ctcctcccac gactctcgcc tgactgctgg tgtgcccgac acgcccaccc
     5221 gcctggtgtt ctctgccctg gggcccacat ctctcagagt gagctggcag gagccgcggt
     5281 gcgagcggcc gctgcagggc tacagtgtgg agtaccagct gctgaacggc ggtgagctgc
     5341 atcggctcaa catccccaac cctgcccaga cctcggtggt ggtggaagac ctcctgccca
     5401 accactccta cgtgttccgc gtgcgggccc agagccagga aggctggggc cgagagcgtg
     5461 agggtgtcat caccattgaa tcccaggtgc acccgcagag cccactgtgt cccctgccag
     5521 gctccgcctt cactttgagc actcccagtg ccccaggccc gctggtgttc actgccctga
     5581 gcccagactc gctgcagctg agctgggagc ggccacggag gcccaatggg gatatcgtcg
     5641 gctacctggt gacctgtgag atggcccaag gaggagggcc agccaccgca ttccgggtgg
     5701 atggagacag ccccgagagc cggctgaccg tgccgggcct cagcgagaac gtgccctaca
     5761 agttcaaggt gcaggccagg accactgagg gcttcgggcc agagcgcgag ggcatcatca
     5821 ccatagagtc ccaggatgga ggacccttcc cgcagctggg cagccgtgcc gggctcttcc
     5881 agcacccgct gcaaagcgag tacagcagca tcaccaccac ccacaccagc gccaccgagc
     5941 ccttcctagt ggatgggctg accctggggg cccagcacct ggaggcaggc ggctccctca
     6001 cccggcatgt gacccaggag tttgtgagcc ggacactgac caccagcgga acccttagca
     6061 cccacatgga ccaacagttc ttccaaactg gaggtggagg ttcagatcca ccggtcgcca
     6121 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     6181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     6241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     6301 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     6361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     6421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     6481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     6541 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     6601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     6661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6721 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6781 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     6841 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     6901 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     6961 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7021 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7081 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     7141 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     7201 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     7261 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     7321 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     7381 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     7441 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     7501 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     7561 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7621 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     7681 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7741 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7861 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7921 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7981 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     8041 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     8101 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     8161 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     8221 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     8281 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     8341 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     8401 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     8461 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     8521 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     8581 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     8641 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8701 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8761 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8821 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8881 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     8941 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9001 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     9061 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     9121 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     9181 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     9241 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     9301 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     9361 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9421 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9481 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9541 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9601 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     9661 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9721 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9781 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9841 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9901 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9961 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10021 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10081 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    10141 ccctgattct gtggataacc gtattaccgc catgcat
//
