LOCUS       V032132                 7165 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032132
VERSION     V032132
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1287
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /transl_table=1
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
                     /label="DsRed2"
     CDS             1348..3825
                     /label="HIF1A(NM_001530)"
                     /note="HIF1A(NM_001530)"
                     /gene="HIF1A"
     polyA_signal    3958..4079
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4086..4541)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4568..4672
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4674..5031
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5066..5860
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6092..6139
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6468..7056
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg
      661 cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      721 ccctacgagg gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc
      781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc
      841 gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      901 atgaacttcg aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc
      961 tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg
     1021 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     1081 ctgaagggcg agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag
     1141 ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac
     1201 gccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc
     1261 accgagggcc gccaccacct gttcctgaga tctcgaggag gcggaggtag tggaggaggg
     1321 ggaagcggag gcggagggag tgaattcatg gagggcgccg gcggcgcgaa cgacaagaaa
     1381 aagataagtt ctgaacgtcg aaaagaaaag tctcgagatg cagccagatc tcggcgaagt
     1441 aaagaatctg aagtttttta tgagcttgct catcagttgc cacttccaca taatgtgagt
     1501 tcgcatcttg ataaggcctc tgtgatgagg cttaccatca gctatttgcg tgtgaggaaa
     1561 cttctggatg ctggtgattt ggatattgaa gatgacatga aagcacagat gaattgcttt
     1621 tatttgaaag ccttggatgg ttttgttatg gttctcacag atgatggtga catgatttac
     1681 atttctgata atgtgaacaa atacatggga ttaactcagt ttgaactaac tggacacagt
     1741 gtgtttgatt ttactcatcc atgtgaccat gaggaaatga gagaaatgct tacacacaga
     1801 aatggccttg tgaaaaaggg taaagaacaa aacacacagc gaagcttttt tctcagaatg
     1861 aagtgtaccc taactagccg aggaagaact atgaacataa agtctgcaac atggaaggta
     1921 ttgcactgca caggccacat tcacgtatat gataccaaca gtaaccaacc tcagtgtggg
     1981 tataagaaac cacctatgac ctgcttggtg ctgatttgtg aacccattcc tcacccatca
     2041 aatattgaaa ttcctttaga tagcaagact ttcctcagtc gacacagcct ggatatgaaa
     2101 ttttcttatt gtgatgaaag aattaccgaa ttgatgggat atgagccaga agaactttta
     2161 ggccgctcaa tttatgaata ttatcatgct ttggactctg atcatctgac caaaactcat
     2221 catgatatgt ttactaaagg acaagtcacc acaggacagt acaggatgct tgccaaaaga
     2281 ggtggatatg tctgggttga aactcaagca actgtcatat ataacaccaa gaattctcaa
     2341 ccacagtgca ttgtatgtgt gaattacgtt gtgagtggta ttattcagca cgacttgatt
     2401 ttctcccttc aacaaacaga atgtgtcctt aaaccggttg aatcttcaga tatgaaaatg
     2461 actcagctat tcaccaaagt tgaatcagaa gatacaagta gcctctttga caaacttaag
     2521 aaggaacctg atgctttaac tttgctggcc ccagccgctg gagacacaat catatcttta
     2581 gattttggca gcaacgacac agaaactgat gaccagcaac ttgaggaagt accattatat
     2641 aatgatgtaa tgctcccctc acccaacgaa aaattacaga atataaattt ggcaatgtct
     2701 ccattaccca ccgctgaaac gccaaagcca cttcgaagta gtgctgaccc tgcactcaat
     2761 caagaagttg cattaaaatt agaaccaaat ccagagtcac tggaactttc ttttaccatg
     2821 ccccagattc aggatcagac acctagtcct tccgatggaa gcactagaca aagttcacct
     2881 gagcctaata gtcccagtga atattgtttt tatgtggata gtgatatggt caatgaattc
     2941 aagttggaat tggtagaaaa actttttgct gaagacacag aagcaaagaa cccattttct
     3001 actcaggaca cagatttaga cttggagatg ttagctccct atatcccaat ggatgatgac
     3061 ttccagttac gttccttcga tcagttgtca ccattagaaa gcagttccgc aagccctgaa
     3121 agcgcaagtc ctcaaagcac agttacagta ttccagcaga ctcaaataca agaacctact
     3181 gctaatgcca ccactaccac tgccaccact gatgaattaa aaacagtgac aaaagaccgt
     3241 atggaagaca ttaaaatatt gattgcatct ccatctccta cccacataca taaagaaact
     3301 actagtgcca catcatcacc atatagagat actcaaagtc ggacagcctc accaaacaga
     3361 gcaggaaaag gagtcataga acagacagaa aaatctcatc caagaagccc taacgtgtta
     3421 tctgtcgctt tgagtcaaag aactacagtt cctgaggaag aactaaatcc aaagatacta
     3481 gctttgcaga atgctcagag aaagcgaaaa atggaacatg atggttcact ttttcaagca
     3541 gtaggaattg gaacattatt acagcagcca gacgatcatg cagctactac atcactttct
     3601 tggaaacgtg taaaaggatg caaatctagt gaacagaatg gaatggagca aaagacaatt
     3661 attttaatac cctctgattt agcatgtaga ctgctggggc aatcaatgga tgaaagtgga
     3721 ttaccacagc tgaccagtta tgattgtgaa gttaatgctc ctatacaagg cagcagaaac
     3781 ctactgcagg gtgaagaatt actcagagct ttggatcaag ttaactgagg atccaccgga
     3841 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     3901 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     3961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     4081 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4141 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     4201 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4261 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4321 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4381 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4441 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4501 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     4561 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4621 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     4681 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4741 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4801 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4861 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4921 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4981 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     5041 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     5101 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5161 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5221 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     5281 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5341 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5401 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5461 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5521 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5581 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5641 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5701 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5761 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5821 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5881 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5941 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6001 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     6061 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     6121 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     6181 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     6241 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     6301 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6361 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6421 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6481 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6541 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6601 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6661 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6721 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6781 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6841 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6901 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6961 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7021 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7081 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7141 tctgtggata accgtattac cgcca
//
