LOCUS       V023721                 8462 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023721
VERSION     V023721
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1378..5022
                     /label="HDAC6(NM_001321226)"
                     /note="HDAC6(NM_001321226)"
                     /gene="HDAC6"
     CDS             5029..5118
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     polyA_signal    5250..5371
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5378..5833)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5860..5964
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5966..6323
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6358..7152
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7384..7431
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7760..8348
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg
     1381 acctcaaccg gccaggattc caccacaacc aggcagcgaa gaagtaggca gaacccccag
     1441 tcgccccctc aggactccag tgtcacttcg aagcgaaata ttaaaaaggg agccgttccc
     1501 cgctctatcc ccaatctagc ggaggtaaag aagaaaggca aaatgaagaa gctcggccaa
     1561 gcaatggaag aagacctaat cgtgggactg caagggatgg atctgaacct tgaggctgaa
     1621 gcactggctg gcactggctt ggtgttggat gagcagttaa atgaattcca ttgcctctgg
     1681 gatgacagct tcccggaagg ccctgagcgg ctccatgcca tcaaggagca actgatccag
     1741 gagggcctcc tagatcgctg cgtgtccttt caggcccggt ttgctgaaaa ggaagagctg
     1801 atgttggttc acagcctaga atatattgat ctgatggaaa caacccagta catgaatgag
     1861 ggagaactcc gtgtcctagc agacacctac gactcagttt atctgcatcc gaactcatac
     1921 tcctgtgcct gcctggcctc aggctctgtc ctcaggctgg tggatgcggt cctgggggct
     1981 gagatccgga atggcatggc catcattagg cctcctggac atcacgccca gcacagtctt
     2041 atggatggct attgcatgtt caaccacgtg gctgtggcag cccgctatgc tcaacagaaa
     2101 caccgcatcc ggagggtcct tatcgtagat tgggatgtgc accacggtca aggaacacag
     2161 ttcaccttcg accaggaccc cagtgtcctc tatttctcca tccaccgcta cgagcagggt
     2221 aggttctggc cccacctgaa ggcctctaac tggtccacca caggtttcgg ccaaggccaa
     2281 ggatatacca tcaatgtgcc ttggaaccag gtggggatgc gggatgctga ctacattgct
     2341 gctttcctgc acgtcctgct gccagtcgcc ctcgagttcc agcctcagct ggtcctggtg
     2401 gctgctggat ttgatgccct gcaaggggac cccaagggtg agatggccgc cactccggca
     2461 gggttcgccc agctaaccca cctgctcatg ggtctggcag gaggcaagct gatcctgtct
     2521 ctggagggtg gctacaacct ccgcgccctg gctgaaggcg tcagtgcttc gctccacacc
     2581 cttctgggag acccttgccc catgctggag tcacctggtg ccccctgccg gagtgcccag
     2641 gcttcagttt cctgtgctct ggaagccctt gagcccttct gggaggttct tgtgagatca
     2701 actgagaccg tggagaggga caacatggag gaggacaatg tagaggagag cgaggaggaa
     2761 ggaccctggg agccccctgt gctcccaatc ctgacatggc cagtgctaca gtctcgcaca
     2821 gggctggtct atgaccaaaa tatgatgaat cactgcaact tgtgggacag ccaccaccct
     2881 gaggtacccc agcgcatctt gcggatcatg tgccgtctgg aggagctggg ccttgccggg
     2941 cgctgcctca ccctgacacc gcgccctgcc acagaggctg agctgctcac ctgtcacagt
     3001 gctgagtacg tgggtcatct ccgggccaca gagaaaatga aaacccggga gctgcaccgt
     3061 gagagttcca actttgactc catctatatc tgccccagta ccttcgcctg tgcacagctt
     3121 gccactggcg ctgcctgccg cctggtggag gctgtgctct caggagaggt tctgaatggt
     3181 gctgctgtgg tgcgtccccc aggacaccac gcagagcagg atgcagcttg cggtttttgc
     3241 tttttcaact ctgtggctgt ggctgctcgc catgcccaga ctatcagtgg gcatgcccta
     3301 cggatcctga ttgtggattg ggatgtccac cacggtaatg gaactcagca catgtttgag
     3361 gatgacccca gtgtgctata tgtgtccctg caccgctatg atcatggcac cttcttcccc
     3421 atgggggatg agggtgccag cagccagatc ggccgggctg cgggcacagg cttcaccgtc
     3481 aacgtggcat ggaacgggcc ccgcatgggt gatgctgact acctagctgc ctggcatcgc
     3541 ctggtgcttc ccattgccta cgagtttaac ccagaactgg tgctggtctc agctggcttt
     3601 gatgctgcac ggggggatcc gctggggggc tgccaggtgt cacctgaggg ttatgcccac
     3661 ctcacccacc tgctgatggg ccttgccagt ggccgcatta tccttatcct agagggtggc
     3721 tataacctga catccatctc agagtccatg gctgcctgca ctcgctccct ccttggagac
     3781 ccaccacccc tgctgaccct gccacggccc ccactatcag gggccctggc ctcaatcact
     3841 gagaccatcc aagtccatcg cagatactgg cgcagcttac gggtcatgaa ggtagaagac
     3901 agagaaggac cctccagttc taagttggtc accaagaagg caccccaacc agccaaacct
     3961 aggttagctg agcggatgac cacacgagaa aagaaggttc tggaagcagg catggggaaa
     4021 gtcacctcgg catcatttgg ggaagagtcc actccaggcc agactaactc agagacagct
     4081 gtggtggccc tcactcagga ccagccctca gaggcagcca cagggggagc cactctggcc
     4141 cagaccattt ctgaggcagc cattggggga gccatgctgg gccagaccac ctcagaggag
     4201 gctgtcgggg gagccactcc ggaccagacc acctcagagg agactgtggg aggagccatt
     4261 ctggaccaga ccacctcaga ggatgctgtt gggggagcca cgctgggcca gactacctca
     4321 gaggaggctg taggaggagc tacactggcc cagaccacct cggaggcagc catggaggga
     4381 gccacactgg accagactac gtcagaggag gctccagggg gcaccgagct gatccaaact
     4441 cctctagcct cgagcacaga ccaccagacc cccccaacct cacctgtgca gggaactaca
     4501 ccccagatat ctcccagtac actgattggg agtctcagga ccttggagct aggcagcgaa
     4561 tctcaggggg cctcagaatc tcaggcccca ggagaggaga acctactagg agaggcagct
     4621 ggaggtcagg acatggctga ttcgatgctg atgcagggat ctaggggcct cactgatcag
     4681 gccatatttt atgctgtgac accactgccc tggtgtcccc atttggtggc agtatgcccc
     4741 atacctgcag caggcctaga cgtgacccaa ccttgtgggg actgtggaac aatccaagag
     4801 aattgggtgt gtctctcttg ctatcaggtc tactgtggtc gttacatcaa tggccacatg
     4861 ctccaacacc atggaaattc tggacacccg ctggtcctca gctacatcga cctgtcagcc
     4921 tggtgttact actgtcaggc ctatgtccac caccaggctc tcctagatgt gaagaacatc
     4981 gcccaccaga acaagtttgg ggaggatatg ccccacccac acgaattcga acaaaaacta
     5041 atatcagagg aagatttgga acagaaacta atcagtgaag aagatttaga gcaaaagtta
     5101 atttccgagg aggacttata ggatccaccg gatctagata actgatcata atcagccata
     5161 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5221 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5281 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5341 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
     5401 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     5461 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     5521 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     5581 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     5641 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     5701 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     5761 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     5821 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5881 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5941 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     6001 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6061 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6121 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6181 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6241 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6301 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     6361 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     6421 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     6481 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     6541 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     6601 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     6661 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     6721 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     6781 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     6841 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     6901 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6961 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     7021 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     7081 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     7141 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     7201 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     7261 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     7321 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     7381 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     7441 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     7501 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     7561 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     7621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     8461 at
//
