LOCUS       V038645                 8304 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038645
VERSION     V038645
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1351..4959
                     /label="Grid2ip(NM_001159321)"
                     /note="Grid2ip(NM_001159321)"
                     /gene="Grid2ip"
     polyA_signal    5092..5213
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5220..5675)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5702..5806
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5808..6165
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6200..6994
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7226..7273
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7602..8190
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagcc atgccagcca ccaatcaagg ctggcccgag
     1381 gacttcggct tccagctggg cggctctggc ccctgcttcg tgatagaggt ggctgaagga
     1441 agcagcgcgc acgctggggg gttgcggccg ggggatcaga tcctggaagt ggaggggctg
     1501 gctgtgggtg gcctgagccg agagcgcatc gtgcgcttgg cacggcgctg ccctcgagtg
     1561 ccacccagcc tcggtgtgct cccaggcccc gagggtggcc ccacagcctt gacagcagcc
     1621 tggctaaccc ggcgatttgg ccgcagcctc ccgctgagcc gcgaactgct gcgtttggct
     1681 ggtggtccca gaccagatgc tgtgcacaga gagcgcaggc gcaaggccca ggagttcagc
     1741 tgccaggtag atgacatctt gggggatcgg ctgacagcca aggagcaggt gtttactgca
     1801 ctgaagcagt tcgctgccga gcaacgggtg gatgagctgg tgtggaccct cactctcgtg
     1861 ctgcccagcg aggcccaggg gccagtcctg gacaacctca ggatctttat tcccaaaaag
     1921 catcgggcgc gcttcgacga ggtggtgtcg cagggtctgc tgggcaagct gtgccgtgct
     1981 cggagggcgc agggcgcaca gcgtttgcgt cggagccgca gcgaggagcg cccggagcgc
     2041 ctcttagtgt ccacgcgcgc tagcgccgct ccccgccgtc cggatgagcc acccccgcgg
     2101 aaggcaacct cgctgctggg cggtcgcact ggccctgggg gtcctcgtag gacggttcga
     2161 gtttataaag gcaacaagag tttcggcttc actctgcgag gccatggacc ggtctggatc
     2221 gaatctgtcc tgcctggcag cccggctgaa aatgcctccc tcaagtcagg tgaccggatc
     2281 ctcttcctca atggattgga catgaggaac tgctcccacg acaaggtggt gtccatgttg
     2341 cagggcagtg gcgccatgcc cacgctggtg gtagaggaag gaccggtccc tttcgccagt
     2401 gattcagatt ctctggattc cccgacccgg gcgtcagctc tcacctcgct gcagtgggtg
     2461 gcggatatcc tgccttccag tatccgagtc caggggagga ccttcagcca gcagctggac
     2521 cacctcctca ctcctcccga gcgctatgga gtctgccgag ctctcgagcg cttctttcag
     2581 cacaggaaca tcgataccct catcgtggat gtctaccccg tcctggacac acccgccaag
     2641 caggtactct ggcagttcct ctaccagctg ctgacctacg aggagcagga gctgtgtcag
     2701 gagaagatcg cgtgcttcct cggctacaca gccatgacgg aacctgaatc ctcactggac
     2761 ctcgagccag agtctacacc agagcccaca ccagagccac aaccacggag ttccctgagg
     2821 gcctcttcca tgtgccgtcg cagcttgcgc tcccagggcc tggagaccag cctcagctgt
     2881 ggtcctggtg actgccctga gatgccccta cctctgatcc caggcgagcg tcaggctggc
     2941 gatggcacat ccctcccgga aacccctaac cccaaaatga tgtcagcggt ctatgcagag
     3001 ctcgagtctc gactgaacag cagcttcaaa gggaagatag ggaccatgtc caaatcccgg
     3061 gcctctccac cagtccccag cctggtcggc acatcagggc ccaggaccct gtctggagtc
     3121 tcctggccca gtgaccggct cctgccttcc ccgtgctatg acccgctgtg ttctgggggt
     3181 ctggcctctc ccagcagctc ggaatcccac ccctacgcca gcttggacag cagcagggcg
     3241 ccctccccac agccaggcct tggatccatc cacgccgaca gccccccaag cccagacccc
     3301 atccggccgc ccagtcgccg gaagctcttc gccttctcac gcccagtccg gagccgagac
     3361 acagatcgct tcctggatgc gctgagcgag cagctgggtc cccgcctcag catcgtggat
     3421 gacttcctga cacccgagaa tgactatgaa gagatgagct tccatgatga ccagggcagc
     3481 tttgtgacca acgagaggag cagcgctagc gagtgtgtca gcagcagcga ggagggcagt
     3541 tctctgacct actcctccat ctccgaccac atccccccac ctccactcag ccccccgccg
     3601 ccaccgcccc tgcctttcca tgaccccaag cctagctccc gcacctccga tggtccccga
     3661 ggtccccctc agtcattgac caaacccctc acccagatca accatccagt cccaccccca
     3721 cccccacccc ctctaccccc acctgtgccc tgtgcacccc ccatgctatc ccgaggcgtg
     3781 ggccaccgcc gcagtgagac cagccatatg agcgtcaagc gcttacgctg ggaacaggtg
     3841 gagaactcag aaggcaccat ctggggtcag cttggggaag actctgacta tgacaagctg
     3901 agtgacatgg tgaaatacct cgacctggag ctccacttcg gcacccagaa acctccgaag
     3961 ccagtgcccg gcccggagcc cttcaggaag aaggaagtgg tggagatctt gtcccacaag
     4021 aaggcctaca atacctccat tctcctagcg cacctgaagc tgacccctgg cgagctgcgg
     4081 caagtgctca tgagcatgga accccggcgc ctggagcctg ctcatctggc gcagctgctg
     4141 ctcttcgctc ctgacgccga cgaggaacag cgatatcagg ctttccgcga ggctccgggt
     4201 cgcctcagtg agccggacca gttcgtcctg cagatgctgt cggttcccga atacaagacc
     4261 cgcctgcgca gtctacactt ccaggctacc ttgcaggaga agacagagga gatacgaggc
     4321 agcctcgagt gtctgcgaca ggcatctctg gagctcaaaa acagccggaa actcgccaag
     4381 atcctggagt ttgtgttggc catgggtaac tatctcaacg atggacagcc caagaccaac
     4441 aagaccactg gcttcaagat caacttcctg accgagctaa actccaccaa gacagtggac
     4501 gggaagtcca cctttctgca catccttgcc aaatcgctaa gccagcactt ccctgagctc
     4561 ctgggctttg cccaggacct gcccactgtg cccctggctg ccaaagtgaa ccagcgagcc
     4621 ctgactggtg acctggctga cctccatgac accgtcagtg agattcaggt ggcctgccag
     4681 agcatggccc cttccagtga ggacaggttt gctgtggtca tggcttcctt tctggaaaca
     4741 gcgcagccag ccctgcgagc gttggatggt ctgcagcgag aggccatgga ggagctaggg
     4801 aaggctctgg ccttcttcgg agaggactcc aaggccacta cctcagaggc cttctttggc
     4861 atcttttcag aattcatgag caaatttgag agagccctca gcgacctgca ggcaggggat
     4921 ggcccacgca gctcagggat ggtgtcaccc ctggcctggt gaggatccac cggatctaga
     4981 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     5041 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     5101 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5161 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     5221 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     5281 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     5341 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     5401 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     5461 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     5521 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     5581 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     5641 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     5701 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5761 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     5821 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5881 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5941 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6001 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6061 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     6181 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     6241 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6301 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6361 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6421 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6481 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6541 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6601 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6661 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6721 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6781 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6841 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6901 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6961 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     7021 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     7081 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7141 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     7201 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     7261 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     7321 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     7381 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     7441 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7501 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7561 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7621 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7681 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7741 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7801 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7861 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7921 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7981 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8041 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8101 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8161 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8221 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8281 gataaccgta ttaccgccat gcat
//
