LOCUS V022649 6183 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022649 VERSION V022649 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1351..2838 /label="GSDME(NM_004403)" /note="GSDME(NM_004403)" /gene="GSDME" polyA_signal 2971..3092 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3099..3554) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3581..3685 /gene="bla" /label="AmpR promoter" promoter 3687..4044 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4079..4873 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5105..5152 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5481..6069 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgagct atgtttgcca aagcaaccag gaattttctt 1381 agagaagttg atgctgatgg tgacctgatt gcagtatcaa atctgaatga ctctgataag 1441 ttacagcttc taagtctggt gacaaaaaag aagagattct ggtgctggca gagacccaag 1501 taccagtttt tatccctcac ccttggcgat gtactcatag aagaccaatt tccgagtcca 1561 gtggtcgtgg agtcggactt tgtgaaatac gagggcaagt ttgcaaacca cgtgagtgga 1621 accctggaga ctgcactggg gaaggtcaag ctgaacctgg ggggcagcag ccgcgtagag 1681 agccagtctt catttggaac cctgaggaag caggaggtgg atttgcagca gctcatcaga 1741 gactctgccg agagaacaat aaatctgaga aaccctgtgc tccagcaggt gctggaagga 1801 aggaatgagg tcctgtgcgt tttgacacag aagatcacga cgatgcagaa gtgtgtgatc 1861 tctgagcaca tgcaggtcga ggagaagtgt ggtggcatcg tgggcatcca gaccaagacg 1921 gtgcaggtgt cagcgacgga ggatgggaat gtcaccaagg actccaacgt ggtgctggag 1981 atcccagctg ccaccaccat tgcctacggt gtcattgagt tatacgtgaa actggacggc 2041 cagttcgagt tctgccttct ccgagggaag caaggtggct tcgagaacaa gaagagaatt 2101 gactctgtct acctggaccc cctggtcttt cgagagtttg cattcataga catgccagat 2161 gctgcgcatg ggatatcttc ccaggatgga ccattaagtg ttttaaagca agcgaccctg 2221 ctcctggaga ggaatttcca tccatttgcg gagctgcctg agccacaaca gacagctttg 2281 agtgacatct tccaggcggt cctatttgat gatgaactac tcatggtcct ggaaccagtg 2341 tgcgatgacc tggtcagcgg cctctcgccc acagtggcgg tgctggggga gctgaagccc 2401 cggcagcagc aggaccttgt ggccttcctg cagctggtgg ggtgcagctt acagggtggg 2461 tgtccgggcc ccgaggatgc aggcagcaag cagctgttta tgacagccta cttcttggtc 2521 agtgccctcg cagaaatgcc agatagcgca gcagctctgc tgggcacttg ctgcaaactc 2581 cagatcattc ccacactgtg ccacttgctt cgtgctctgt ctgatgatgg agtatctgat 2641 cttgaagacc caaccttgac tcccctgaaa gatacagaaa ggtttgggat tgtgcagcgc 2701 ttgtttgcct cagctgacat tagtctggag agactgaagt catctgtgaa agctgtcatt 2761 ctgaaggact ctaaagtctt cccactgctt ctttgtataa ccctgaatgg actctgtgct 2821 ttaggcagag aacattcatg aggatccacc ggatctagat aactgatcat aatcagccat 2881 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 2941 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 3001 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 3061 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 3121 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 3181 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 3241 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 3301 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 3361 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 3421 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 3481 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 3541 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 3601 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 3661 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 3721 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3781 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3841 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3961 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 4021 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 4081 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4141 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4201 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4261 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4321 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 4381 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 4441 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 4501 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 4561 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 4621 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4681 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4741 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4801 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4861 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 4921 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 4981 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 5041 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 5101 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 5161 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 5221 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 5281 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 5341 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 5401 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 5461 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 5521 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 5581 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 5641 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5701 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5761 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5821 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5881 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5941 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 6001 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 6061 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 6121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 6181 cat //