LOCUS       V021477                 5767 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021477
VERSION     V021477
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             627..653
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             654..1679
                     /label="GALM(NM_138801)"
                     /note="GALM(NM_138801)"
                     /gene="GALM"
     CDS             1713..2432
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2555..2676
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2683..3138)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3165..3269
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3271..3628
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3663..4457
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4689..4736
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5065..5653
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgtacc catacgacgt accagattac gctatggctt
      661 cggtgaccag ggccgtgttt ggagagctgc cctcgggagg agggacagtg gagaagttcc
      721 agctgcagtc agacctcttg agagtggaca tcatctcctg gggctgcacg atcacagccc
      781 tagaggtcaa agacaggcag gggagagcct cggacgtggt gcttggcttc gccgagttgg
      841 aaggatacct ccaaaagcag ccatactttg gagcagttat tgggagggtg gccaaccgaa
      901 tcgccaaagg aaccttcaag gtggatggga aggagtatca cctggccatt aacaaggaac
      961 ccaacagtct gcatggagga gtcagagggt ttgataaagt gctctggacc cctcgggtgc
     1021 tgtcaaatgg cgtccagttc tcgcgcatca gtccagatgg tgaagaaggc taccccggag
     1081 agttaaaagt ctgggtgaca tacaccctgg atggcggaga gctcatagtc aactacagag
     1141 cacaagccag tcaggccaca ccagtcaacc tgaccaacca ttcttacttc aacctggcag
     1201 gccaggcttc cccaaatata aatgaccatg aagtcaccat agaagcggat acttatttgc
     1261 ctgtggatga aaccctgatt cctacaggag aagttgcccc agtgcaaggc actgcattcg
     1321 acctgagaaa gccagtggag cttggaaaac acctgcagga cttccatctc aatggttttg
     1381 accacaattt ctgtctgaag ggatctaaag aaaagcattt ttgtgcaagg gtgcatcatg
     1441 ctgcaagcgg gcgggtacta gaagtataca ccacccagcc cggggtccag ttttacacgg
     1501 gcaacttcct ggatggcaca ttaaagggca agaatggagc tgtctatccc aagcactccg
     1561 gtttctgcct ggagactcag aactggcctg atgcagtcaa tcagccccgc ttccctcctg
     1621 tgctgctgag gcctggtgag gagtatgacc acaccacctg gttcaagttt tctgtggctg
     1681 gaggaggggg aagcgatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     1741 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1801 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1861 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     1921 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1981 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2041 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2101 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2161 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2221 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2281 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     2341 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2401 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag
     2461 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2521 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2581 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2641 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     2701 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     2761 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     2821 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     2881 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     2941 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3001 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3061 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3121 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3181 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3241 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3301 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3361 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3421 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3541 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3601 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     3661 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3721 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3781 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3841 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3901 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3961 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4021 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4081 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4141 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4201 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4261 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4321 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4381 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4441 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4501 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4561 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4621 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     4681 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     4741 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     4801 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     4861 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     4921 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4981 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5041 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5101 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5161 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5221 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5281 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5341 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5401 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5461 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5521 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5581 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5641 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5701 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5761 catgcat
//
