LOCUS       V018359                 5151 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018359
VERSION     V018359
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..1086
                     /label="Fgf2(NM_008006)"
                     /note="Fgf2(NM_008006)"
                     /gene="Fgf2"
     CDS             1138..1818
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="wild-type DsRed"
                     /transl_table=1
                     /translation="M,V,RSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHN
                     TVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGE
                     IHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEG
                     RHHLFL*"
                     /label="DsRed1"
     polyA_signal    1939..2060
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2067..2522)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        2549..2653
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        2655..3012
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3047..3841
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4073..4120
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4449..5037
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatggct gccagcggca tcacctcgct tcccgcactg
      661 ccggaggacg gcggcgccgc cttcccacca ggccacttca aggaccccaa gcggctctac
      721 tgcaagaacg gcggcttctt cctgcgcatc catcccgacg gccgcgtgga tggcgtccgc
      781 gagaagagcg acccacacgt caaactacaa ctccaagcag aagagagagg agttgtgtct
      841 atcaagggag tgtgtgccaa ccggtacctt gctatgaagg aagatggacg gctgctggct
      901 tctaagtgtg ttacagaaga gtgtttcttc tttgaacgac tggaatctaa taactacaat
      961 acttaccggt cacggaaata ctccagttgg tatgtggcac tgaaacgaac tgggcagtat
     1021 aaactcggat ccaaaacggg acctggacag aaggccatac tgtttcttcc aatgtctgct
     1081 aagagcagaa ttctgcagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg
     1141 gtgcgctcct ccaagaacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
     1201 accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1261 cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     1321 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
     1381 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1441 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac
     1501 aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1561 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     1621 atccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc
     1681 tacatggcca agaagcccgt gcagctgccc ggctactact acgtggactc caagctggac
     1741 atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc
     1801 caccacctgt tcctgtagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     1861 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     1921 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1981 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2041 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     2101 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2161 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     2221 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     2281 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     2341 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     2401 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     2461 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     2521 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2581 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2641 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     2701 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2761 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2821 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2881 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2941 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3001 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     3061 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3121 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     3181 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     3241 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     3301 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     3361 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     3421 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     3481 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     3541 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     3601 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     3661 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     3721 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     3781 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     3841 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     3901 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     3961 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     4021 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     4081 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     4141 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     4201 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     4261 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     4321 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4381 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4441 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4501 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4561 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4621 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4681 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4741 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4801 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4861 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4921 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4981 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5041 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5101 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
