LOCUS       V021874                 8341 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021874
VERSION     V021874
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             624..4253
                     /EC_number=""
                     /codon_start=1
                     /gene=""
                     /note=""
                     /product="precursor of human epidermal growth factor
                     receptor"
                     /protein_id=""
                     /transl_table=1
                     /translation="MRPSGTAGAALLALLAALCPASRA,LEEKKVCQGTSNKLTQLGTF
                     EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLEN
                     LQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESI
                     QWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSG
                     RCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVN
                     PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG
                     IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTV
                     KEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEIS
                     DGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCW
                     GPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRG
                     PDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLE
                     GCPTNGPKIPS,IATGMVGALLLLLVVALGIGLFM,RRRHIVRKRTLRRLLQERELVEP
                     LTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA
                     TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNI
                     GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKE
                     YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL
                     EKGERLPQPPICTIDVYMIMRKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER
                     MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS
                     TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS
                     VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH
                     QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
                     /label="EGFR"
     CDS             4287..5006
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    5129..5250
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5257..5712)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5739..5843
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5845..6202
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6237..7031
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7263..7310
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7639..8227
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgcgac cctccgggac ggccggggca gcgctcctgg
      661 cgctgctggc tgcgctctgc ccggcgagtc gggctctgga ggaaaagaaa gtttgccaag
      721 gcacgagtaa caagctcacg cagttgggca cttttgaaga tcattttctc agcctccaga
      781 ggatgttcaa taactgtgag gtggtccttg ggaatttgga aattacctat gtgcagagga
      841 attatgatct ttccttctta aagaccatcc aggaggtggc tggttatgtc ctcattgccc
      901 tcaacacagt ggagcgaatt cctttggaaa acctgcagat catcagagga aatatgtact
      961 acgaaaattc ctatgcctta gcagtcttat ctaactatga tgcaaataaa accggactga
     1021 aggagctgcc catgagaaat ttacaggaaa tcctgcatgg cgccgtgcgg ttcagcaaca
     1081 accctgccct gtgcaacgtg gagagcatcc agtggcggga catagtcagc agtgactttc
     1141 tcagcaacat gtcgatggac ttccagaacc acctgggcag ctgccaaaag tgtgatccaa
     1201 gctgtcccaa tgggagctgc tggggtgcag gagaggagaa ctgccagaaa ctgaccaaaa
     1261 tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa gtcccccagt gactgctgcc
     1321 acaaccagtg tgctgcaggc tgcacaggcc cccgggagag cgactgcctg gtctgccgca
     1381 aattccgaga cgaagccacg tgcaaggaca cctgcccccc actcatgctc tacaacccca
     1441 ccacgtacca gatggatgtg aaccccgagg gcaaatacag ctttggtgcc acctgcgtga
     1501 agaagtgtcc ccgtaattat gtggtgacag atcacggctc gtgcgtccga gcctgtgggg
     1561 ccgacagcta tgagatggag gaagacggcg tccgcaagtg taagaagtgc gaagggcctt
     1621 gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa agactcactc tccataaatg
     1681 ctacgaatat taaacacttc aaaaactgca cctccatcag tggcgatctc cacatcctgc
     1741 cggtggcatt taggggtgac tccttcacac atactcctcc tctggatcca caggaactgg
     1801 atattctgaa aaccgtaaag gaaatcacag ggtttttgct gattcaggct tggcctgaaa
     1861 acaggacgga cctccatgcc tttgagaacc tagaaatcat acgcggcagg accaagcaac
     1921 atggtcagtt ttctcttgca gtcgtcagcc tgaacataac atccttggga ttacgctccc
     1981 tcaaggagat aagtgatgga gatgtgataa tttcaggaaa caaaaatttg tgctatgcaa
     2041 atacaataaa ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa attataagca
     2101 acagaggtga aaacagctgc aaggccacag gccaggtctg ccatgccttg tgctcccccg
     2161 agggctgctg gggcccggag cccagggact gcgtctcttg ccggaatgtc agccgaggca
     2221 gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc aagggagttt gtggagaact
     2281 ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc catgaacatc acctgcacag
     2341 gacggggacc agacaactgt atccagtgtg cccactacat tgacggcccc cactgcgtca
     2401 agacctgccc ggcaggagtc atgggagaaa acaacaccct ggtctggaag tacgcagacg
     2461 ccggccatgt gtgccacctg tgccatccaa actgcaccta cggatgcact gggccaggtc
     2521 ttgaaggctg tccaacgaat gggcctaaga tcccgtccat cgccactggg atggtggggg
     2581 ccctcctctt gctgctggtg gtggccctgg ggatcggcct cttcatgcga aggcgccaca
     2641 tcgttcggaa gcgcacgctg cggaggctgc tgcaggagag ggagcttgtg gagcctctta
     2701 cacccagtgg agaagctccc aaccaagctc tcttgaggat cttgaaggaa actgaattca
     2761 aaaagatcaa agtgctgggc tccggtgcgt tcggcacggt gtataaggga ctctggatcc
     2821 cagaaggtga gaaagttaaa attcccgtcg ctatcaagga attaagagaa gcaacatctc
     2881 cgaaagccaa caaggaaatc ctcgatgaag cctacgtgat ggccagcgtg gacaaccccc
     2941 acgtgtgccg cctgctgggc atctgcctca cctccaccgt gcaactcatc acgcagctca
     3001 tgcccttcgg ctgcctcctg gactatgtcc gggaacacaa agacaatatt ggctcccagt
     3061 acctgctcaa ctggtgtgtg cagatcgcaa agggcatgaa ctacttggag gaccgtcgct
     3121 tggtgcaccg cgacctggca gccaggaacg tactggtgaa aacaccgcag catgtcaaga
     3181 tcacagattt tgggctggcc aaactgctgg gtgcggaaga gaaagaatac catgcagaag
     3241 gaggcaaagt gcctatcaag tggatggcat tggaatcaat tttacacaga atctataccc
     3301 accagagtga tgtctggagc tacggggtga ccgtttggga gttgatgacc tttggatcca
     3361 agccatatga cggaatccct gccagcgaga tctcctccat cctggagaaa ggagaacgcc
     3421 tccctcagcc acccatatgt accatcgatg tctacatgat catgcgcaag tgctggatga
     3481 tagacgcaga tagtcgccca aagttccgtg agttgatcat cgaattctcc aaaatggccc
     3541 gagaccccca gcgctacctt gtcattcagg gggatgaaag aatgcatttg ccaagtccta
     3601 cagactccaa cttctaccgt gccctgatgg atgaagaaga catggacgac gtggtggatg
     3661 ccgacgagta cctcatccca cagcagggct tcttcagcag cccctccacg tcacggactc
     3721 ccctcctgag ctctctgagt gcaaccagca acaattccac cgtggcttgc attgatagaa
     3781 atgggctgca aagctgtccc atcaaggaag acagcttctt gcagcgatac agctcagacc
     3841 ccacaggcgc cttgactgag gacagcatag acgacacctt cctcccagtg cctgaataca
     3901 taaaccagtc cgttcccaaa aggcccgctg gctctgtgca gaatcctgtc tatcacaatc
     3961 agcctctgaa ccccgcgccc agcagagacc cacactacca ggacccccac agcactgcag
     4021 tgggcaaccc cgagtatctc aacactgtcc agcccacctg tgtcaacagc acattcgaca
     4081 gccctgccca ctgggcccag aaaggcagcc accaaattag cctggacaac cctgactacc
     4141 agcaggactt ctttcccaag gaagccaagc caaatggcat ctttaagggc tccacagctg
     4201 aaaatgcaga atacctaagg gtcgcgccac aaagcagtga atttattgga gcaggaggag
     4261 ggggaagcga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4321 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4381 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4441 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     4501 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4561 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4621 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4681 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4741 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     4801 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4861 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     4921 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4981 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     5041 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     5101 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     5161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5221 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     5281 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     5341 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     5401 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     5461 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     5521 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     5581 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     5641 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     5701 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5761 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5821 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     5881 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5941 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6001 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6061 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6121 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6181 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     6241 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     6301 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     6361 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     6421 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     6481 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     6541 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     6601 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     6661 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     6721 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     6781 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     6841 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     6901 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     6961 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     7021 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     7081 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7141 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7201 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     7261 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     7321 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     7381 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     7441 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     7501 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7561 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7621 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7681 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7741 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7801 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7861 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7921 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7981 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8041 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8101 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8161 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8221 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8281 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     8341 t
//
