LOCUS       V022335                 7335 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022335
VERSION     V022335
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1393..3990
                     /label="DMGDH(NM_013391)"
                     /note="DMGDH(NM_013391)"
                     /gene="DMGDH"
     polyA_signal    4123..4244
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4251..4706)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4733..4837
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4839..5196
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5231..6025
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6257..6304
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6633..7221
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttcgaattc tgatgctccg tcccggcgcg cagctgctgc ggggcctcct gctgcggagc
     1441 tgcccgctgc agggctcccc cgggcgcccg cgctctgtct gcggccggga aggagaggaa
     1501 aaaccaccct tatctgcaga aacacaatgg aaagacagag cagaaacagt gataattgga
     1561 ggtggctgtg ttggtgtgag tctggcttat cacctggcca aagcagggat gaaagatgtg
     1621 gtcctgctgg agaaatcaga gctcacggct ggatctacct ggcacgcagc aggtttaaca
     1681 acttactttc atcctggaat aaacttgaag aaaatacatt atgatagcat caaactttat
     1741 gagaaactgg aagaagaaac tggtcaggtg gtgggattcc atcagccagg tagtatcaga
     1801 cttgctacca cccctgtaag ggtagatgaa tttaaatatc aaatgactcg gactggctgg
     1861 catgcaacag aacagtatct cattgaacct gaaaaaattc aagagatgtt ccctttactc
     1921 aacatgaata aggttttagc tggattgtat aatcctggag atggtcacat tgatccttat
     1981 tctctaacta tggcactggc tgctggggct aggaaatgtg gtgccctttt aaaatatcct
     2041 gcaccagtaa cttctctgaa agccaggtca gatggaacat gggacgttga aacaccacag
     2101 gggtctatga gagcaaatag aattgtgaat gctgcaggat tttgggctcg tgaagtaggt
     2161 aaaatgattg gactagaaca tcctctcatt ccggttcaac atcaatatgt tgttacatcg
     2221 actatatctg aagtgaaagc tttgaaacga gaactgcctg tgctccgtga cctggaagga
     2281 tcatattatc tccgacagga aagggatggg cttttgtttg gtccatatga aagtcaagag
     2341 aaaatgaaag ttcaggactc ctgggtcacc aatggagttc ctccaggttt tggaaaggaa
     2401 ctctttgagt ctgatctaga tcgaatcatg gaacacatca aagctgccat ggaaatggtt
     2461 cctgtcttga aaaaggctga catcatcaat gttgtcaatg gtcctatcac gtattctcct
     2521 gacattctgc ctatggtggg gccccatcag ggggtcagaa actactgggt ggctataggc
     2581 tttggatatg gcataatcca cgctggtggg gtagggaaat atctcagtga ctggatcctg
     2641 catggagaac ctccttttga tctgatagaa ttggatccta atcgctatgg caaatggaca
     2701 acaacccagt acactgaggc caaagcaaga gaatcatatg gattcaacaa tattgttggt
     2761 tatcctaaag aagaacggtt tgctgggagg ccgactcaac gagtcagtgg gctctatcaa
     2821 aggctggagt ctaagtgttc catggggttc catgctggct gggagcagcc gcactggttc
     2881 tacaaaccag gccaggacac tcagtacagg ccaagttttc gccgcacaaa ctggtttgag
     2941 cctgtgggct cggagtataa acaggttatg caaagagtag cggtaactga cctatcacca
     3001 tttggcaagt ttaacatcaa aggccaagat tccattagac tactggacca tctctttgca
     3061 aatgtcattc caaaggtggg ttttacaaat ataagtcaca tgttaacacc caagggtcga
     3121 gtgtatgctg agctgactgt ttctcaccaa tctcctgggg agtttctttt aattactggc
     3181 tctggatcag aacttcatga tcttagatgg attgaagaag aagcagtcaa aggtggatat
     3241 gatgttgaaa ttaaaaacat aactgatgag cttggagttc ttggagttgc tgggccacag
     3301 gcaagaaagg tccttcagaa actgacctct gaagatctta gtgatgatgt tttcaagttt
     3361 cttcaaacca agtccttaaa ggtttccaac attcctgtca ctgctattag gatatcttat
     3421 actggtgagc tgggttggga gctgtatcac agaagagaag attctgtggc gctgtatgac
     3481 gctatcatga atgcaggcca ggaggaggga atcgacaatt ttggaaccta tgccatgaat
     3541 gccttacgcc tggagaaagc cttcagagcc tgggggttag agatgaactg tgatacaaat
     3601 cctttggaag ctggactgga atattttgtg aagttaaata agccagcaga cttcatagga
     3661 aagcaagcac tgaaacagat taaagccaag gggctgaaac gaagactggt ctgcctcacc
     3721 ttggcaacgg atgatgttga tccagaggga aatgaaagca tctggtacaa tggcaaggtg
     3781 gttggcaaca cgacatctgg aagctatagc tacagcatcc agaagagtct ggctttcgca
     3841 tatgtccctg tacaactaag tgaagtggga cagcaagtgg aagttgaact attaggcaaa
     3901 aattacccag cagtcatcat acaagaacct ttggtattga ccgaaccaac cagaaaccgg
     3961 cttcagaaaa aaggtggaaa ggacaaaact tgaggatcca ccggatctag ataactgatc
     4021 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4081 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4141 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4201 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
     4261 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     4321 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     4381 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     4441 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     4501 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4561 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4621 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4681 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     4741 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4801 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     4861 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4921 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4981 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5041 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5101 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5161 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     5221 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5281 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5341 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5401 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5461 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5521 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5581 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5641 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5701 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     5761 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5821 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5881 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5941 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6001 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6061 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6121 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6181 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     6241 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     6301 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     6361 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     6421 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     6481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7321 attaccgcca tgcat
//
