LOCUS       V033965                 6740 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033965
VERSION     V033965
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced GFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="mEGFP"
     CDS             1378..3363
                     /label="DDX3X(NM_001356)"
                     /note="DDX3X(NM_001356)"
                     /gene="DDX3X"
     CDS             3370..3396
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3528..3649
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3656..4111)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4138..4242
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4244..4601
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4636..5430
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5662..5709
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6038..6626
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggt ggaggtggat caggtggtgg aggatctatg
     1381 agtcatgtgg cagtggaaaa tgcgctcggg ctggaccagc agtttgctgg cctagacctg
     1441 aactcttcag ataatcagag tggaggaagt acagccagca aagggcgcta tattcctcct
     1501 catttaagga accgagaagc tactaaaggt ttctacgata aagacagttc agggtggagt
     1561 tctagcaaag ataaggatgc gtatagcagt tttggatctc gtagtgattc aagagggaag
     1621 tctagcttct tcagtgatcg tggaagtgga tcaaggggaa ggtttgatga tcgtggacgg
     1681 agtgattacg atggcattgg cagccgtggt gacagaagtg gctttggcaa atttgaacgt
     1741 ggtggaaaca gtcgctggtg tgacaaatca gatgaagatg attggtcaaa accactccca
     1801 ccaagtgaac gcttggaaca ggaactcttt tctggaggca acactgggat taattttgag
     1861 aaatacgatg acattccagt tgaggcaaca ggcaacaact gtcctccaca tattgaaagt
     1921 ttcagtgatg ttgagatggg agaaattatc atgggaaaca ttgagcttac tcgttatact
     1981 cgcccaactc cagtgcaaaa gcatgctatt cctattatca aagagaaaag agacttgatg
     2041 gcttgtgccc aaacagggtc tggaaaaact gcagcatttc tgttgcccat cttgagtcag
     2101 atttattcag atggtccagg cgaggctttg agggccatga aggaaaatgg aaggtatggg
     2161 cgccgcaaac aatacccaat ctccttggta ttagcaccaa cgagagagtt ggcagtacag
     2221 atctacgagg aagccagaaa attttcatac cgatctagag ttcgtccttg cgtggtttat
     2281 ggtggtgccg atattggtca gcagattcga gacttggaac gtggatgcca tttgttagta
     2341 gccactccag gacgtctagt ggatatgatg gaaagaggaa agattggatt agacttttgc
     2401 aaatacttgg tgttagatga agctgatcgg atgttggata tggggtttga gcctcagatt
     2461 cgtagaatag tcgaacaaga tactatgcct ccaaagggtg tccgccacac tatgatgttt
     2521 agtgctactt ttcctaagga aatacagatg ctggctcgtg atttcttaga tgaatatatc
     2581 ttcttggctg taggaagagt tggctctacc tctgaaaaca tcacacagaa agtagtttgg
     2641 gtggaagaat cagacaaacg gtcatttctg cttgacctcc taaatgcaac aggcaaggat
     2701 tcactgacct tagtgtttgt ggagaccaaa aagggtgcag attctctgga ggatttctta
     2761 taccatgaag gatacgcatg taccagcatc catggagacc gttctcagag ggatagagaa
     2821 gaggcccttc accagttccg ctcaggaaaa agcccaattt tagtggctac agcagtagca
     2881 gcaagaggac tggacatttc aaatgtgaaa catgttatca attttgactt gccaagtgat
     2941 attgaagaat atgtacatcg tattggtcgt acgggacgtg taggaaacct tggcctggca
     3001 acctcattct ttaacgagag gaacataaat attactaagg atttgttgga tcttcttgtt
     3061 gaagctaaac aagaagtgcc gtcttggtta gaaaacatgg cttatgaaca ccactacaag
     3121 ggtagcagtc gtggacgttc taagagtagc agatttagtg gagggtttgg tgccagagac
     3181 taccgacaaa gtagcggtgc cagcagttcc agcttcagca gcagccgcgc aagcagcagc
     3241 cgcagtggcg gaggtggcca cggtagcagc agaggatttg gtggaggtgg ctatggaggc
     3301 ttttacaaca gtgatggata tggaggaaat tataactccc agggggttga ctggtggggt
     3361 aacctcgagt acccatacga cgtacctgat tatgcatagg atccaccgga tctagataac
     3421 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3481 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3541 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3601 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt
     3661 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3721 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3781 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3841 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3901 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3961 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4021 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4081 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     4141 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4201 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     4261 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4321 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4381 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4441 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4501 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4561 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     4621 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4681 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4741 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4801 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     4861 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4921 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4981 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5041 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5101 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5161 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5221 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5281 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5341 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5401 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     5461 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     5521 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5581 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     5641 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     5701 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     5761 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     5821 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     5881 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5941 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6001 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6061 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6181 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6721 accgtattac cgccatgcat
//
