LOCUS       V039008                 6800 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039008
VERSION     V039008
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1378..3360
                     /label="Cd180(NM_008533)"
                     /note="Cd180(NM_008533)"
                     /gene="Cd180"
     CDS             3367..3456
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     polyA_signal    3588..3709
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3716..4171)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4198..4302
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4304..4661
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4696..5490
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5722..5769
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6098..6686
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg
     1381 gctccggaca tcagctgctt ctttttagtg gccctcttct tggccagttg ccgagccacc
     1441 acatcctcag atcagaagtg cattgagaaa gaagtcaaca aaacatacaa ctgtgaaaat
     1501 ttaggtctca atgaaattcc tggcacttta ccaaactcaa cagaatgttt ggagttcagc
     1561 tttaatgtct tgcctaccat tcaaaacacg accttcagca gacttatcaa tctcaccttc
     1621 ctggatttaa ccaggtgcca gatttactgg atacatgaag atactttcca aagccaacat
     1681 cggttagaca cacttgtact aaccgcaaat cccctgatat ttatggcaga gacagcactt
     1741 agtgggccta aggcattgaa gcatctgttc ttcatccaaa caggaatatc cagtattgat
     1801 tttatcccac tgcacaatca aaaaaccttg gaaagtctct atctcggaag caaccatatt
     1861 tcctccatta agctccccaa aggtttccca acagagaagc tgaaggtcct ggattttcag
     1921 aataatgcta tccattacct gtctaaagaa gatatgagct ctctccagca agccactaat
     1981 ctgagcctta acttaaatgg aaatgacatt gcaggaatag agctgggggc tttcgactca
     2041 gctgtcttcc aaagtttaaa ctttggaggg actcagaact tgctagttat cttcaagggt
     2101 ttgaagaact ctacgatcca gtctctttgg ctggggacat ttgaggacat ggatgacgaa
     2161 gatattagtc ctgccgtgtt tgagggtctc tgtgaaatgt ctgtggagag catcaaccta
     2221 cagaagcatt atttcttcaa catttcctcc aacacattcc attgcttcag tggcctccag
     2281 gaactggacc taacagccac tcacctgagt gaattgcctt ctggacttgt gggactaagc
     2341 acacttaaga aattagttct cagtgcaaat aagtttgaga atttgtgcca aatcagtgct
     2401 tccaatttcc cctcccttac tcacctttcc atcaagggca acacgaagag acttgagctc
     2461 ggtactggct gtttagaaaa cctagaaaat ctccgcgaac ttgacctcag ccatgatgac
     2521 attgaaactt ctgattgttg caacctgcaa ctccgaaacc tgtctcactt acagagcctg
     2581 aacttgagct acaatgaacc cctgagcctc aagacggagg cattcaaaga atgccctcag
     2641 ctagaactcc tagatttggc atttactcga ctaaaggtga aagatgcaca gagtcccttc
     2701 cagaacctcc atcttttgaa ggtgctgaat ctctcccaca gcctccttga catcagcagt
     2761 gagcagctct tcgatggcct gccagcactc cagcatttga acttacaggg aaatcacttt
     2821 ccaaaaggga atattcaaaa gaccaactca cttcagacac tgggaagact agaaatcctg
     2881 gttttatcct tttgtgatct ctcctccatt gaccagcacg ccttcaccag tctgaagatg
     2941 atgaatcatg tagacctgag tcacaacagg ctgacatcca gtagcattga ggctcttagt
     3001 catcttaagg ggatctacct caatctggcc tccaatcgca tcagcatcat cctacccagt
     3061 ctcctcccca tcttgtccca gcagaggacc attaatttaa gacaaaatcc ccttgactgc
     3121 acttgctcaa acatttactt tttagaatgg tacaaagaaa acatgcaaaa actcgaggac
     3181 acagaggaca ctctttgcga aaatccccca ttgctgaggg gagtcaggct ctccgatgtc
     3241 acgctatcct gtagtatggc agctgtgggc attttctttc ttattgtatt cttgctcgtg
     3301 tttgctattt tgctgatttt tgcagtgaaa tattttctca gatggaaata ccaacacatt
     3361 gaattcgaac aaaaactaat atcagaggaa gatttggaac agaaactaat cagtgaagaa
     3421 gatttagagc aaaagttaat ttccgaggag gacttatagg atccaccgga tctagataac
     3481 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3541 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt
     3721 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3781 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3841 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3901 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3961 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4021 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4081 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4141 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     4201 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4261 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     4321 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4381 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4441 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4501 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4561 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4621 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     4681 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4741 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4801 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4861 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     4921 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4981 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5041 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5101 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5161 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5221 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5281 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5341 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5401 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5461 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     5521 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     5581 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5641 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     5701 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     5761 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     5821 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     5881 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     5941 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6001 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6061 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6121 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6181 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6241 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6301 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6361 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6421 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6481 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6541 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6601 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6661 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6721 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6781 accgtattac cgccatgcat
//
