LOCUS V025001 5705 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025001 VERSION V025001 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1320 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1354..2334 /label="CREB1(NM_004379)" /note="CREB1(NM_004379)" /gene="CREB1" CDS 2335..2361 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 2493..2614 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2621..3076) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3103..3207 /gene="bla" /label="AmpR promoter" promoter 3209..3566 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3601..4395 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4627..4674 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5003..5591 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagg aggaggggga agcatgacca tggaatctgg agccgagaac 1381 cagcagagtg gagatgcagc tgtaacagaa gctgaaaacc aacaaatgac agttcaagcc 1441 cagccacaga ttgccacatt agcccaggta tctatgccag cagctcatgc aacatcatct 1501 gctcccaccg taactctagt acagctgccc aatgggcaga cagttcaagt ccatggagtc 1561 attcaggcgg cccagccatc agttattcag tctccacaag tccaaacagt tcagatttca 1621 actattgcag aaagtgaaga ttcacaggag tcagtggata gtgtaactga ttcccaaaag 1681 cgaagggaaa ttctttcaag gaggccttcc tacaggaaaa ttttgaatga cttatcttct 1741 gatgcaccag gagtgccaag gattgaagaa gagaagtctg aagaggagac ttcagcacct 1801 gccatcacca ctgtaacggt gccaactcca atttaccaaa ctagcagtgg acagtatatt 1861 gccattaccc agggaggagc aatacagctg gctaacaatg gtaccgatgg ggtacagggc 1921 ctgcaaacat taaccatgac caatgcagca gccactcagc cgggtactac cattctacag 1981 tatgcacaga ccactgatgg acagcagatc ttagtgccca gcaaccaagt tgttgttcaa 2041 gctgcctctg gagacgtaca aacataccag attcgcacag cacccactag cactattgcc 2101 cctggagttg ttatggcatc ctccccagca cttcctacac agcctgctga agaagcagca 2161 cgaaagagag aggtccgtct aatgaagaac agggaagcag ctcgagagtg tcgtagaaag 2221 aagaaagaat atgtgaaatg tttagaaaac agagtggcag tgcttgaaaa tcaaaacaag 2281 acattgattg aggagctaaa agcacttaag gacctttact gccacaaatc agattaccca 2341 tacgacgtac cagattacgc ttaggatcca ccggatctag ataactgatc ataatcagcc 2401 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 2461 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 2521 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 2581 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt 2641 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 2701 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 2761 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 2821 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 2881 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 2941 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 3001 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 3061 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 3121 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 3181 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt 3241 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 3301 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 3361 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 3421 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 3481 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 3541 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc 3601 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 3661 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 3721 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 3781 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 3841 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 3901 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 3961 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 4021 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 4081 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 4141 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 4201 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 4261 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 4321 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 4381 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 4441 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 4501 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 4561 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 4621 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac 4681 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 4741 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 4801 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac 4861 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 4921 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 4981 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 5041 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 5101 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 5161 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 5221 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 5281 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 5341 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 5401 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 5461 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 5521 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 5581 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 5641 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca 5701 tgcat //