LOCUS       V024130                 5997 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024130
VERSION     V024130
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             601..2574
                     /label="CPT2(NM_000098)"
                     /note="CPT2(NM_000098)"
                     /gene="CPT2"
     CDS             2581..2646
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    2785..2906
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2913..3368)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3395..3499
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3501..3858
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3893..4687
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4919..4966
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5295..5883
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggtgcccc gcctgctgct gcgcgcctgg ccccggggcc ccgcggttgg tccgggagcc
      661 cccagtcggc ccctcagcgc cggctccggg cccggccagt acctgcagcg cagcatcgtg
      721 cccaccatgc actaccagga cagcctgccc aggctgccta ttcccaaact tgaagacacc
      781 attaggagat acctcagtgc acagaagcct ctcttgaatg atggccagtt caggaaaaca
      841 gaacaatttt gcaagagttt tgaaaatggg attggaaaag aactgcatga gcagctggtt
      901 gctctggaca aacagaataa acatacaagc tacatttcgg gaccctggtt tgatatgtac
      961 ctatctgctc gagactccgt tgttctgaac tttaatccat ttatggcttt caatcctgac
     1021 ccaaaatctg agtataatga ccagctcacc cgggcaacca acatgactgt ttctgccatc
     1081 cggtttctga agacactccg ggctggcctt ctggagccag aagtgttcca cttgaaccct
     1141 gcaaaaagtg acactatcac cttcaagaga ctcatacgct ttgtgccttc ctctctgtcc
     1201 tggtatgggg cctacctggt caatgcgtat cccctggata tgtcccagta ttttcggctt
     1261 ttcaactcaa ctcgtttacc caaacccagt cgggatgaac tcttcactga tgacaaggcc
     1321 agacacctcc tggtcctaag gaaaggaaat ttttatatct ttgatgtcct ggatcaagat
     1381 gggaacattg tgagcccctc ggaaatccag gcacatctga agtacattct ctcagacagc
     1441 agccccgccc ccgagtttcc cctggcatac ctgaccagtg agaaccgaga catctgggca
     1501 gagctcaggc agaagctgat gagtagtggc aatgaggaga gcctgaggaa agtggactcg
     1561 gcagtgttct gtctctgcct agatgacttc cccattaagg accttgtcca cttgtcccac
     1621 aatatgctgc atggggatgg cacaaaccgc tggtttgata aatcctttaa cctcattatc
     1681 gccaaggatg gctctactgc cgtccacttt gagcactctt ggggtgatgg tgtggcagtg
     1741 ctcagatttt ttaatgaagt atttaaagac agcactcaga cccctgccgt cactccacag
     1801 agccagccag ctaccactga ctctactgtc acggtgcaga aactcaactt cgagctgact
     1861 gatgccttaa agactggcat cacagctgct aaggaaaagt ttgatgccac catgaaaacc
     1921 ctcactattg actgcgtcca gtttcagaga ggaggcaaag aattcctgaa gaagcaaaag
     1981 ctgagccctg acgcagttgc ccagctggca ttccagatgg ccttcctgcg gcagtacggg
     2041 cagacagtgg ccacctacga gtcctgtagc actgccgcat tcaagcacgg ccgcactgag
     2101 accatccgcc cggcctccgt ctatacaaag aggtgctctg aggcctttgt cagggagccc
     2161 tccaggcaca gtgctggtga gcttcagcag atgatggttg agtgctccaa gtaccatggc
     2221 cagctgacca aagaagcagc aatgggccag ggctttgacc gacacttgtt tgctctgcgg
     2281 catctggcag cagccaaagg gatcatcttg cctgagctct acctggaccc tgcatacggg
     2341 cagataaacc acaatgtcct gtccacgagc acactgagca gcccagcagt gaaccttggg
     2401 ggctttgccc ctgtggtctc tgatggcttt ggtgttgggt atgctgttca tgacaactgg
     2461 ataggctgca atgtctcttc ctacccaggc cgcaatgccc gggagtttct ccaatgtgtg
     2521 gagaaggcct tagaagacat gtttgatgcc ttagaaggca aatccatcaa aagtggatcc
     2581 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     2641 gacaagtgag ggcccgttta aacgcggccg cgactctaga tcataatcag ccataccaca
     2701 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2761 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2821 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2881 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     2941 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3001 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3061 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3121 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3181 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3241 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3301 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3361 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3481 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3541 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3601 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3661 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3721 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3781 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3841 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     3901 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3961 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4021 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4081 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4141 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4201 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4261 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4321 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4381 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4441 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4501 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4561 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4621 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4681 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4741 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     4801 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     4861 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4921 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     4981 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5041 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5101 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5161 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5221 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5281 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5341 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5401 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     5461 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5521 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5581 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5641 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5701 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5761 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5821 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5881 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5941 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
