LOCUS       V027823                 7245 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027823
VERSION     V027823
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..3900
                     /label="Atg9a(NM_001014218)"
                     /note="Atg9a(NM_001014218)"
                     /gene="Atg9a"
     polyA_signal    4033..4154
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4161..4616)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4643..4747
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4749..5106
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5141..5935
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6167..6214
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6543..7131
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatggcac agtttgacac tgaataccag cgcctagagg cctcttatag cgattcaccc
     1441 ccaggggaag aggacctgtt ggtgcatgtg gctgagggga gcaaatcacc ttggcaccac
     1501 attgaaaacc ttgacctctt cttctctcga gtttataatc tacatcagaa gaatgggttc
     1561 acatgtatgc tcattggaga gatttttgaa ctcatgcagt tcctctttgt ggttgccttc
     1621 accaccttcc tggttagctg tgtggactat gacatcctat ttgccaacaa gatggtgaac
     1681 cacagtctgc atcctaccga gcctgtcaag gtcactctgc cagatgcctt tttgcctgcc
     1741 caagtttgca gtgccaggat tcaggaaaac ggctccctca tcaccatcct ggtcatcgct
     1801 ggtgtcttct ggatccaccg gcttatcaag tttatctata acatttgctg ctattgggag
     1861 atccactcct tctacctaca tgctctgcgg atcccaatgt ctgcccttcc gtattgcacg
     1921 tggcaggaag tgcaggcccg gattgtgcag acccagaaag agcatcagat ctgcatccac
     1981 aagcgtgagc tgacagagtt ggacatctac catcgcatcc tccgtttcca gaactacatg
     2041 gtggcactgg tgaacaaatc cctcttgcct ctgcgcttcc gtctgcccgg cctcggagag
     2101 gttgtcttct tcacccgtgg cctcaagtac aactttgagc tcatcctctt ctggggaccc
     2161 ggctctctgt ttctcaatga atggagcctc aaggccgagt acaaacgtgg agggcaacgg
     2221 ctagagctgg cccagcgtct cagcaaccgc atcctgtgga ttggcatcgc caacttcctg
     2281 ctctgtcccc tcatcctcat ctggcagatc ctctatgcct tcttcagcta tgccgaggtg
     2341 ctgaagagag agccgggagc cctgggagcg cgttgctggt cactctatgg ccgttgctac
     2401 ctccgccact tcaatgagct agaacacgag ctgcagtccc gcctcaaccg aggatacaag
     2461 cccgcctcca agtacatgaa ttgcttcttg tcaccgctac tgactctgct ggccaagaat
     2521 ggtgccttct tcgcgggctc tatcctggcc gtgcttattg ctctcacgat ctacgatgaa
     2581 gatgtgttgg ctgtggagca tgtcctcacc actgtcaccc tactgggagt cacggtgact
     2641 gtgtgcaggt ctttcatccc agaccagcac atggtgttct gccctgagca gctgctccga
     2701 gtgatccttg cccacatcca ctacatgcct gaccactggc agggtaatgc ccaccgctcg
     2761 cagacccggg acgagtttgc ccagctcttc cagtacaagg cagtgttcat cttggaggag
     2821 ttgctgagtc ccattgttac acccctcatt ctcatcttct gcctacgccc acgggccctg
     2881 gagatcatag acttcttccg aaactttact gtggaggtcg tcggtgtggg ggacacctgc
     2941 tcctttgcgc agatggacgt tcgccagcac ggtcatcctc agtggctgtc tggagggcag
     3001 actgaggcct cagtgtacca gcaagccgag gatgggaaga ccgagttgtc actcatgcac
     3061 tttgccatca ccaatcctgg ctggcagccc cctcgtgaga gcacagcctt cctgggcttc
     3121 ctcaaggagc aggtgcagcg agatggagcg gctgctggcc ttgcccaggg tggtctgctc
     3181 ccagagaatg cccttttcac atccatccag tccttacagt ctgagtctga gccactgagc
     3241 ctcattgcaa atgtggtagc aggctcgtcc tgccgaggac ccccactgtc cagagacctg
     3301 cagggttcca ggcacagggc tgatgttgct tctgccctta gatccttctc ccctctacag
     3361 cctggacagg cccctcaagg ccgtgttccc agtaccatga caggctctgg agtggacgcc
     3421 aggacagcca gctccgggag cagcgtgtgg gaaggacagc tgcaaagcct ggtgctgtct
     3481 gaatatgcgt ccactgagat gagccttcat gccctctata tgcaccagct ccacaagcag
     3541 cagacccagg ctgagcccga gcggcatgtg tggcaccgcc gggagagtga tgagagtgga
     3601 gagagtgccc ctgaagaggg gggagagggt gcccgggccc cccaacccat cccacgctct
     3661 gccagctatc cctgtgctac tccccggcct ggagcacctg agaccaccgc cctgcatggg
     3721 ggcttccaga ggcgctacgg gggcatcaca gatcctggca cagtgccccg tgctccctct
     3781 cacttctcca ggctgccctt gggaggatgg gcagaagatg ggcagccagc atcaagacac
     3841 ccagagccgg tgccagagga gggctcagag gatgaactgc cccctcaagt gcacaaggta
     3901 tagggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     3961 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     4021 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     4081 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     4141 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     4201 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     4261 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4321 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     4381 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     4441 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     4501 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4561 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     4621 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4681 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4741 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     4801 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4861 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4921 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4981 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5041 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5101 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     5161 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5221 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5281 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     5341 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5401 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5461 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5521 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5581 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5641 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     5701 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5761 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5821 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5881 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5941 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6001 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6061 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     6121 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     6181 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     6241 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     6301 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     6361 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     6421 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6481 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6541 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6601 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6661 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6721 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6781 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6841 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6901 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6961 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7021 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7081 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7141 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7201 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
