LOCUS       V021940                 7326 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021940
VERSION     V021940
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1393..3981
                     /label="ADAM15(NM_207197)"
                     /note="ADAM15(NM_207197)"
                     /gene="ADAM15"
     polyA_signal    4114..4235
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4242..4697)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4724..4828
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4830..5187
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5222..6016
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6248..6295
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6624..7212
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttcgaattc tcatgcggct ggcgctgctc tgggccctgg ggctcctggg cgcgggcagc
     1441 cctctgcctt cctggccgct cccaaatata ggtggcactg aggagcagca ggcagagtca
     1501 gagaaggccc cgagggagcc cttggagccc caggtccttc aggacgatct cccaattagc
     1561 ctcaaaaagg tgcttcagac cagtctgcct gagcccctga ggatcaagtt ggagctggac
     1621 ggtgacagtc atatcctgga gctgctacag aatagggagt tggtcccagg ccgcccaacc
     1681 ctggtgtggt accagcccga tggcactcgg gtggtcagtg agggacacac tttggagaac
     1741 tgctgctacc agggaagagt gcggggatat gcaggctcct gggtgtccat ctgcacctgc
     1801 tctgggctca gaggcttggt ggtcctgacc ccagagagaa gctataccct ggagcagggg
     1861 cctggggacc ttcagggtcc tcccattatt tcgcgaatcc aagatctcca cctgccaggc
     1921 cacacctgtg ccctgagctg gcgggaatct gtacacactc agaagccacc agagcacccc
     1981 ctgggacagc gccacattcg ccggaggcgg gatgtggtaa cagagaccaa gactgtggag
     2041 ttggtgattg tggctgatca ctcggaggcc cagaaatacc gggacttcca gcacctgcta
     2101 aaccgcacac tggaagtggc cctcttgctg gacacattct tccggcccct gaatgtacga
     2161 gtggcactag tgggcctgga ggcctggacc cagcgtgacc tggtggagat cagcccaaac
     2221 ccagctgtca ccctcgaaaa cttcctccac tggcgcaggg cacatttgct gcctcgattg
     2281 ccccatgaca gtgcccagct ggtgactggt acttcattct ctgggcctac ggtgggcatg
     2341 gccattcaga actccatctg ttctcctgac ttctcaggag gtgtgaacat ggaccactcc
     2401 accagcatcc tgggagtcgc ctcctccata gcccatgagt tgggccacag cctgggcctg
     2461 gaccatgatt tgcctgggaa tagctgcccc tgtccaggtc cagccccagc caagacctgc
     2521 atcatggagg cctccacaga cttcctacca ggcctgaact tcagcaactg cagccgacgg
     2581 gccctggaga aagccctcct ggatggaatg ggcagctgcc tcttcgaacg gctgcctagc
     2641 ctacccccta tggctgcttt ctgcggaaat atgtttgtgg agccgggcga gcagtgtgac
     2701 tgtggcttcc tggatgactg cgtcgatccc tgctgtgatt ctttgacctg ccagctgagg
     2761 ccaggtgcac agtgtgcatc tgacggaccc tgttgtcaaa attgccagct gcgcccgtct
     2821 ggctggcagt gtcgtcctac cagaggggat tgtgacttgc ctgaattctg cccaggagac
     2881 agctcccagt gtccccctga tgtcagccta ggggatggcg agccctgcgc tggcgggcaa
     2941 gctgtgtgca tgcacgggcg ttgtgcctcc tatgcccagc agtgccagtc actttgggga
     3001 cctggagccc agcccgctgc gccactttgc ctccagacag ctaatactcg gggaaatgct
     3061 tttgggagct gtgggcgcaa ccccagtggc agttatgtgt cctgcacccc tagagatgcc
     3121 atttgtgggc agctccagtg ccagacaggt aggacccagc ctctgctggg ctccatccgg
     3181 gatctactct gggagacaat agatgtgaat gggactgagc tgaactgcag ctgggtgcac
     3241 ctggacctgg gcagtgatgt ggcccagccc ctcctgactc tgcctggcac agcctgtggc
     3301 cctggcctgg tgtgtataga ccatcgatgc cagcgtgtgg atctcctggg ggcacaggaa
     3361 tgtcgaagca aatgccatgg acatggggtc tgtgacagca acaggcactg ctactgtgag
     3421 gagggctggg caccccctga ctgcaccact cagctcaaag caaccagctc cctgaccaca
     3481 gggctgctcc tcagcctcct ggtcttattg gtcctggtga tgcttggtgc cagctactgg
     3541 taccgtgccc gcctgcacca gcgactctgc cagctcaagg gacccacctg ccagtacagg
     3601 gcagcccaat ctggtccctc tgaacggcca ggacctccgc agagggccct gctggcacga
     3661 ggcactaagc aggctagtgc tctcagcttc ccggcccccc cttccaggcc gctgccgcct
     3721 gaccctgtgt ccaagagact ccaggctgag ctggctgacc gacccaatcc ccctacccgc
     3781 cctctgcccg ctgacccggt ggtgagaagc ccgaagtctc aggggccagc caagccccca
     3841 cccccaagga agccactgcc tgccgacccc cagggccggt gcccatcggg tgacctgccc
     3901 ggcccagggg ctggaatccc gcccctagtg gtaccctcca gaccagcgcc accgcctccg
     3961 acagtgtcct cgctctacct ctgaggatcc accggatcta gataactgat cataatcagc
     4021 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4081 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4141 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4201 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     4261 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4321 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4381 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4441 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4501 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4561 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4621 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4681 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4741 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4801 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     4861 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4921 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4981 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     5041 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5101 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5161 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     5221 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5281 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5341 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5401 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5461 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5521 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5581 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5641 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5701 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     5761 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5821 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5881 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5941 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6001 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     6061 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6121 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6181 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     6241 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     6301 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     6361 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     6421 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     6481 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6541 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6601 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6661 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6721 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6781 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6841 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6901 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6961 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7021 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7081 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7141 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7201 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7261 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7321 atgcat
//
