LOCUS       V028190                 6945 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028190
VERSION     V028190
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..3600
                     /label="ACSL5(NM_016234)"
                     /note="ACSL5(NM_016234)"
                     /gene="ACSL5"
     polyA_signal    3733..3854
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3861..4316)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4343..4447
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4449..4806
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4841..5635
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5867..5914
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6243..6831
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatggacg ctctgaagcc accctgtctc tggaggaacc acgagcgagg gaagaaggac
     1441 agggactcgt gtggcaggaa gaactcagag ccgggaagcc cccattcact agaagcactg
     1501 agagatgcgg ccccctcgca gggtctgaat ttcctgctgc tgttcacaaa gatgcttttt
     1561 atctttaact ttttgttttc cccacttccg accccggcgt tgatctgcat cctgacattt
     1621 ggagctgcca tcttcttgtg gctgatcacc agacctcaac ccgtcttacc tcttcttgac
     1681 ctgaacaatc agtctgtggg aattgaggga ggagcacgga agggggtttc ccagaagaac
     1741 aatgacctaa caagttgctg cttctcagat gccaagacta tgtatgaggt tttccaaaga
     1801 ggactcgctg tgtctgacaa tgggccctgc ttgggatata gaaaaccaaa ccagccctac
     1861 agatggctat cttacaaaca ggtgtctgat agagcagagt acctgggttc ctgtctcttg
     1921 cataaaggtt ataaatcatc accagaccag tttgtcggca tctttgctca gaataggcca
     1981 gagtggatca tctccgaatt ggcttgttac acgtactcta tggtagctgt acctctgtat
     2041 gacaccttgg gaccagaagc catcgtacat attgtcaaca aggctgatat cgccatggtg
     2101 atctgtgaca caccccaaaa ggcattggtg ctgataggga atgtagagaa aggcttcacc
     2161 ccgagcctga aggtgatcat ccttatggac ccctttgatg atgacctgaa gcaaagaggg
     2221 gagaagagtg gaattgagat cttatcccta tatgatgctg agaacctagg caaagagcac
     2281 ttcagaaaac ctgtgcctcc tagcccagaa gacctgagcg tcatctgctt caccagtggg
     2341 accacaggtg accccaaagg agccatgata acccatcaaa atattgtttc aaatgctgct
     2401 gcctttctca aatgtgtgga gcatgcttat gagcccactc ctgatgatgt ggccatatcc
     2461 tacctccctc tggctcatat gtttgagagg attgtacagg ctgttgtgta cagctgtgga
     2521 gccagagttg gattcttcca aggggatatt cggttgctgg ctgacgacat gaagactttg
     2581 aagcccacat tgtttcccgc ggtgcctcga ctccttaaca ggatctacga taaggtacaa
     2641 aatgaggcca agacaccctt gaagaagttc ttgttgaagc tggctgtttc cagtaaattc
     2701 aaagagcttc aaaagggtat catcaggcat gatagtttct gggacaagct catctttgca
     2761 aagatccagg acagcctggg cggaagggtt cgtgtaattg tcactggagc tgcccccatg
     2821 tccacttcag tcatgacatt cttccgggca gcaatgggat gtcaggtgta tgaagcttat
     2881 ggtcaaacag aatgcacagg tggctgtaca tttacattac ctggggactg gacatcaggt
     2941 cacgttgggg tgcccctggc ttgcaattac gtgaagctgg aagatgtggc tgacatgaac
     3001 tactttacag tgaataatga aggagaggtc tgcatcaagg gtacaaacgt gttcaaagga
     3061 tacctgaagg accctgagaa gacacaggaa gccctggaca gtgatggctg gcttcacaca
     3121 ggagacattg gtcgctggct cccgaatgga actctgaaga tcatcgaccg taaaaagaac
     3181 attttcaagc tggcccaagg agaatacatt gcaccagaga agatagaaaa tatctacaac
     3241 aggagtcaac cagtgttaca aatttttgta cacggggaga gcttacggtc atccttagta
     3301 ggagtggtgg ttcctgacac agatgtactt ccctcatttg cagccaagct tggggtgaag
     3361 ggctcctttg aggaactgtg ccaaaaccaa gttgtaaggg aagccatttt agaagacttg
     3421 cagaaaattg ggaaagaaag tggccttaaa acttttgaac aggtcaaagc catttttctt
     3481 catccagagc cattttccat tgaaaatggg ctcttgacac caacattgaa agcaaagcga
     3541 ggagagcttt ccaaatactt tcggacccaa attgacagcc tgtatgagca catccaggat
     3601 tagggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     3661 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     3721 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3781 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3841 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3901 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3961 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4021 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     4081 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     4141 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     4201 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4261 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     4321 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4381 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4441 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     4501 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4561 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4621 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4681 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4741 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4801 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4861 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4921 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4981 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     5041 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5101 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5161 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5221 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5281 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5341 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     5401 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5461 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5521 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5581 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5641 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5701 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5761 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     5821 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     5881 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     5941 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     6001 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     6061 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     6121 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6181 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6241 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6301 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6361 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6421 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6481 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6541 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6601 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6661 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6721 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6781 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6841 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6901 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
