LOCUS       62056_7475        9067 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        632..650
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2916..2927
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     polyA_signal    6063..6287
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    6506..6639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6676..6692)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6700..6716)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6724..6754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6769..6790)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7078..7666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7840..8697)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8698..8802)
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaagct gtaatacgac tcactatagg gatcccagga
      661 gttccggtcc ggcagagatc gcggagagac gcagaacgca gcccgctcct ccagggccct
      721 ccaggccctc cggccccggg ccggcgggtg aactgggggg ccccgggaca ggccgagccc
      781 tctgccctgc agataacgga ggcctctgct gtggctgccc actggctgtg cccgcccact
      841 ggctgtgccc agaccttgaa gccgcagcga acctctcttt cccaccccac ctcggtgact
      901 aatggcggcc gtggcgtctc ccagcccgga ccccgccggc acccgggtct cccgacccaa
      961 gcctcgacga aacccccgca gagccgccgg gacgcagcgc ctttgggcgg cgctgggcgt
     1021 ggtgggccgg gaagtatggc ggcagctcga acgccgcgcg gcggaggcca ttaaggcgtg
     1081 gacggcccgg gaaggcggcc tagggacgca agcaggctcg gccgcctctt taggccacgg
     1141 agccgcgcag atccggttcc cgggtgacca ctctgtcgcc attgggcgag acctacctag
     1201 tcctgacgac aacggacaaa ggccttaagg ggcctggaag gtgagcgaag tcccgaacga
     1261 cgacgggtgg aacggttagc ggccatcggg cggttggtct tcattctacc agactttgct
     1321 gtcggaagag agaaatggta gaatgacagg ccacgtttgg cccgttggaa atgcccacca
     1381 ccctctggga agatttactg gccgtttatg gaaggcctgt gtatataata tgaaaaagct
     1441 gctctcaact ccaccccaac cttttaatag aaaacatttg tcacatctag cccttctaga
     1501 tggaaagagg ttgccgacgt atgataaaat agagttagaa agttacacat cttgtaaatt
     1561 ctcatttgtt taaaagaaat catagaaaat acatgtcttc tggagatgac ttttggaaat
     1621 ggagttgtta agacggcctc tggaagcgat acgtccacgt ttgttaagtg ggttagatga
     1681 catggagctg gaagacctga gaaggaagag aagaaggttc tatgctagac tggtcatatt
     1741 tagaagacat tttcatattc tatccattgt tttgtgtgca ttttattcct cactactgtg
     1801 tatatagttg acaatgctaa gcttttttga aatgtctctt ctttttagat gttctgaagt
     1861 gcctgatatg ttaaaattag aggtagcaaa atcacatttt gtaaatacct ttttgttaca
     1921 attcatagga aatatttttg ggggggaatg gccaaatcac ctgttgagta atactcattg
     1981 tgtttgtgca gtggttcagg ggaggagaga ggagggggag gtgcagagag ctctatgcca
     2041 tcctgtttac agcgaggcaa gatgaatcat tatgtctgtg cattttgttt tacttatctg
     2101 tgtatatagt gtacataaag gacagacgag tcctaattga caacatctag tctttctgga
     2161 tgttaaagag gttgccagtg tatgacaaaa gtagagttag taaactaata tattttgtac
     2221 attttgtttt acaagtccta ggaaagattg tcttctgaaa atttgatgtc ttctgggttg
     2281 atggagatgg gaagggttct aggccagaat gttcacattt ggaagactct ttcaaattat
     2341 aactgttgtt acatgtttgc agtttattca agactgctgt atacatagta gacaaattaa
     2401 ctccttactt gaaacatcta gtctatctag atgtttagaa gtgcccgatg tatgttaaat
     2461 gtataggtag taaaatacca ctttgtaaat atctttttgc taaaattcat aggaaatgct
     2521 tttggaaatt gaattgtgaa gccacctttg tgaacagtat agtaatgtct atacttgttc
     2581 aatagtttag aggaggtagg agggaagaaa ttgcaaaagg taatattact agtgtgttca
     2641 tacttggaca ttttcagaca ccatttttct atatgttttg tgcattttgt tttgctctgt
     2701 atatagtata tataatggac aaatagtcct aatttttcaa catctagtct ctagatgtta
     2761 aagaggttgc cagtgtatga caaaggagta aaattagcat attttgtaca ctttgtgttg
     2821 aaattcgtag gaaaacttgt cttctgtaaa gacttttgca taggaatttg tttgaccatc
     2881 tctaagcatt acacgtgcct gtacttgtcc actggattga aggcagagaa ggaagggagg
     2941 agggaatgat tcaaggccaa aatggccaca tttagaagat acctcagatg ataaccattg
     3001 ttatgtgtgt gcaattttat ttaacagtgc tgtgtatgtg gtggacaagt tatatgaaat
     3061 atctagtctt tctagatatt tggaagtgct tgatgtattt aaaagtggta gtagaataac
     3121 actttgtaaa tagcttttaa aaactgatgg gaaatgctgt ttggaagtgg aattgttgaa
     3181 ccacctggga ggtgggaggg aagaaattgc aaatggtgtt ttgccattgt ttattagaaa
     3241 atttcagctt aatccattgt gtatatgtta catgcatttc atttaacttt gctatactgt
     3301 atatattgta tatataacgg acaaattagt cccgatttta taatatctag tctctagata
     3361 ttaaagaggt tgccaatgta tgacagaagt agagttagta aactaacaca ttttgtacac
     3421 tttgttaaaa tttgtagaaa ggctgtcttc tgaaaaggac ttttggaagt gagataacat
     3481 cagctctaag tgacacgtgc ctatatccat caggttggtg gtggagagga gttggaagga
     3541 atgaagggtt ctagaccaga atgttcgtat ttagaagaca ctatcagata taaccattgt
     3601 tacatgtgtg tagtttattc aaccctactg tgtatatagc ggacaaactt aagtccttat
     3661 ttgaaacatc tagtctttct agatgtttag aagtgcacaa agtatgttaa aagtagaggt
     3721 agtaaataac acattttgta gctatccttt tgatatgaaa tattgtcttg gaaattgatc
     3781 aattctctga gcagtaccca ttttgatatt tgtgctggtt cagggggaag gaggagcaca
     3841 aagtgcaaag ggctttctac cagtgtccag tgtgtttatg aggaggcaca ttgaccattg
     3901 tcccttatgt ctgcattttc atttactgtg ctgtgtatat agtgtatata agcggacata
     3961 ggagtcctaa tttacgtcta gtcgatgtta aaaaggttgc cagtatatga caaaagtaga
     4021 attagtaaac tactacattg agtacacttt gtgttaaaat tcatagggaa gacttcttaa
     4081 aaacaagtga aattgttaaa acccccccta agcattacag atggcttata gctgtccacg
     4141 gggttggtag aggtgggaaa gggaagggtt ctaggccaga atgttcctat ttagaagaca
     4201 ctcaaattac agtctgtgtt atgtatgtat accatttatt caatgctact gtgtatataa
     4261 tggaaaactt aagtccagtt tgaaacatct agtctttcta ggtgtttaaa agtgtacaac
     4321 ggcctgtcgc agtggcgcat gcctgtaatc ccagcacttt gggaggccga ggcaggcgga
     4381 tcacgaggtc aagagatcag gaccatcttg gccaacatgg tgaaacccca tctttactaa
     4441 aaatacaaaa attagctggt cgtggtggtg cccacctgta gccccagtta ctcgagaggc
     4501 tgaggcagga gaatcgcttg aacttgggag gcggaagttg cagtgagcca agatcgcacc
     4561 actgcactcc agcctggcga cagagcgagg ctccgtttca aaaaaaaaag tgcacaatgt
     4621 aggttaacag tagagggctt aagtaacacc cctctaagca tttgttttca gtacttccta
     4681 ggagtggttg catttgggaa tggaattgtt aaaacttgat gcttaggagc gaatgcagac
     4741 tattcattgg gtgtttgggg tgggggaagg gggggtgggc agaggaggta tgcagggaga
     4801 ggggttctgt gctcctgaga ttagttcaga tggtctaacc attgttctat atgtgcattt
     4861 tagttaatat tgtgtattaa aggataagtc ttaatgctca aagtatgtta aaaatagatg
     4921 tagtaaatca gtccctttgt gaatgtcctt ttgttagttt ttaggaaggc ctgtcctctg
     4981 ggagtgacct ttattagtcc accccttgga gctagacatc ctgtacttag tcacggggat
     5041 ggtggaagag ggagaagagg aagggtgaag ggaagggctc tttgctagta tctccatatc
     5101 tagacgatgg ttttagatga taaccacagg tctacaagag cgtttttagt aaagtgcctg
     5161 tgttcattgt ggacaaagtt attattttgc aacatctaag ctttacgaat ggggtgacaa
     5221 cttatgataa aaactagagc tagtgaatta gcctatttgt aaataccttt gttataattg
     5281 ataggataca tcttggacat ggaattgtta agccacctct gagcagtgta tgtcaggact
     5341 tgttcattag gttggcagca gaggggcaga aggaattata caggtagaga tgtatgcaga
     5401 tgtgtccata tatgtccata tttacatttt gatagccatt gatgtatgca tctcttggct
     5461 gtactataag aacacattaa ttcaatggaa atacactttg ctaatatttt aatggtatag
     5521 atctgctaat gaattctctt aaaaacatac tgtattctgt tgctgtgtgt ttcattttaa
     5581 attgagcatt aagggaatgc agcatttaaa tcagaactct gccaatgctt ttatctagag
     5641 gcgtgttgcc atttttgtct tatatgaaat ttctgtccca agaaaggcag gattacatct
     5701 tttttttttt tagcagtttg agttggtgta gtgtattctt ggttatcaga atactcatat
     5761 agctttggga ttttgaattg gtaaatattc atgatgtgtg aaaaatcatg atacatactg
     5821 tacagtctca gtcccataaa attggatgtt gtgcctacac acaggatcta gaagaatatg
     5881 tcaaactata aactgcttgt gattgtgaat gactttgttc tttgcttgtg tttttcaatt
     5941 tcctataatg cacatactaa cttttaaaaa ataaaggtta ttttaaaagc ctgtacggta
     6001 ccgcgatcgc aagcttgtcc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     6061 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6121 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6181 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6241 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6301 aagaaccagc tggggctcta gggggtatcc ccgcgggact ctggggttcg aaatgaccga
     6361 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6421 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6481 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6541 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6601 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6661 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6721 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6781 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6841 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6901 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6961 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7021 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7081 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7141 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7201 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7261 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7321 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7381 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7441 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7501 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7561 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7621 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7681 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7741 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7801 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7861 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7921 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7981 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8041 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8101 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8161 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8221 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8281 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8341 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8401 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8461 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8521 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8581 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8641 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8701 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8761 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8821 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     8881 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     8941 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     9001 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     9061 acgcgtt
//
