LOCUS       V020763                 4733 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020763
VERSION     V020763
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             58..720
                     /label="NDFIP1(NM_030571)"
                     /note="NDFIP1(NM_030571)"
                     /gene="NDFIP1"
     CDS             778..1029
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /transl_table=1
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
                     /label="VC155"
     polyA_signal    1044..1178
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(1289..1305)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    1313..1329
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(1337..1367)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    1382..1403
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(1691..2279)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(2450..3310)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(3311..3415)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     3889..3905
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        3932..4235
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        4236..4439
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          4577..4673
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
ORIGIN
        1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattcccatg
       61 gcgttggcgt tggcggcgct ggcggcggtc gagccggcct gcggcagccg gtaccagcag
      121 ttgcagaatg aagaagagtc tggagaacct gaacaggctg caggtgatgc tcctccacct
      181 tacagcagca tttctgcaga gagcgcagca tattttgact acaaggatga gtctgggttt
      241 ccaaagcccc catcttacaa tgtagctaca acactgccca gttatgatga agcggagagg
      301 accaaggctg aagctactat ccctttggtt cctgggagag atgaggattt tgtgggtcgg
      361 gatgattttg atgatgctga ccagctgagg ataggaaatg atgggatttt catgttaact
      421 tttttcatgg cattcctctt taactggatt gggtttttcc tgtctttttg cctgaccact
      481 tcagctgcag gaaggtatgg ggccatttca ggatttggtc tctctctaat taaatggatc
      541 ctgattgtca ggttttccac ctatttccct ggatattttg atggtcagta ctggctctgg
      601 tgggtgttcc ttgttttagg ctttctcctg tttctcagag gatttatcaa ttatgcaaaa
      661 gttcggaaga tgccagaaac tttctcaaat ctccccagga ccagagttct ctttatttat
      721 ggtacccgtc cggcgtgcaa aatcccgaac gacctgaaac agaaagtcat gaaccacgac
      781 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
      841 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      901 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc
      961 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1021 ctgtacaagt aagcggccgc ggggatccag acatgataag atacattgat gagtttggac
     1081 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1141 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1201 ttatgtttca ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgatct
     1261 gcaggcatgc tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1321 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1381 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1441 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1501 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1561 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1621 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1681 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1861 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2101 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2221 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2341 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2401 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2461 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2521 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2581 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2641 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2701 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2761 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2821 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2881 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2941 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3001 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3061 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3121 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3181 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3241 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3301 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3361 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3421 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3481 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     3541 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     3601 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     3661 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     3721 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     3781 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     3841 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     3901 ccagtgagtt cgagcttgca tgcctgcagg tcgttacata acttacggta aatggcccgc
     3961 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4021 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4081 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     4141 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4201 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     4261 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4321 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     4381 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     4441 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     4501 gacaccggga ccgatccagc ctccggactc tagaggatcc ggtactagag gaactgaaaa
     4561 accagaaagt taactggtaa gtttagtctt tttgtctttt atttcaggtc ccggatccgg
     4621 tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc taggcctgta
     4681 cggaagtgtt acttctgctc taaaagctgc ggaattgtac ccgcgggccc acc
//
