LOCUS       pCMV-Myc.        3791 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing an N-terminally Myc-tagged protein in 
            mammalian cells. pCMV-Myc-N is identical but contains a complete Myc
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This plasmid contains a truncated Myc tag.
FEATURES             Location/Qualifiers
     source          1..3791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             829..831
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             841..867
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /note="Myc (truncated)"
                     /note="The first amino acid of the standard Myc tag is
                     absent."
                     /translation="QKLISEEDL"
     misc_feature    872..926
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    complement(930..1064)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1175..1191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1199..1215)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1223..1253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1268..1289)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1577..2165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2339..3196)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3197..3301)
                     /label=AmpR promoter
     primer_bind     3775..3791
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg
      841 cagaagctga tctcagagga ggacctgctt atggccatgg aggcccgaat tcggtcgacc
      901 gagatctctc gaggtaccgc ggccgcgggg atccagacat gataagatac attgatgagt
      961 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1021 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1081 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcggat cctctagagt
     1141 cgatctgcag gcatgctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1201 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1261 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1321 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2641 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2701 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2761 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2821 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2881 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2941 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3001 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3061 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3121 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3181 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3241 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3301 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3361 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     3421 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3481 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     3541 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     3601 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     3661 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     3721 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     3781 cgacggccag t
//
