LOCUS       62056_7400        4528 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(1..49)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(208..411)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        412..715
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        716..919
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             987..1703
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1829..1950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1979..2083
                     /label=AmpR promoter
     promoter        2085..2442
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2477..3268
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3503..3550
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3879..4467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
       61 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcccccattt ccagtctaga
      121 tgagaattcg atgcttgagc tcgagatctg agtccggtag cgctagccga taagccagta
      181 agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg
      241 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
      301 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
      361 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccgttacata
      421 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      481 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      541 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      601 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      661 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      721 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      781 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      841 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      901 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cctgcagtcg acggtaccgc
      961 gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1021 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1081 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1141 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     1201 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1261 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1321 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1381 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1441 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1501 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1561 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1621 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1681 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     1741 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1801 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1861 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1921 tggtttgtcc aaactcatca atgtatctta agcaggtggc acttttcggg gaaatgtgcg
     1981 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2041 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     2101 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2161 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2221 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     2281 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2341 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2401 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     2461 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     2521 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     2581 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     2641 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     2701 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     2761 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     2821 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     2881 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     2941 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3001 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3061 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3121 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3181 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3241 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     3301 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     3361 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     3421 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     3481 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     3541 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     3601 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     3661 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     3721 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3781 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4021 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4501 gccttttgct ggccttttgc tcacatgt
//
