LOCUS       62056_7345        4728 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    591..671
                     /label=MCS
                     /note="multiple cloning site"
     CDS             681..1391
                     /codon_start=1
                     /label=KillerRed
                     /note="red fluorescent protein KillerRed, a
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /translation="SEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFNVH
                     AVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDGTM
                     TSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLAFI
                     GFTTADGGLMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQMRDTSDKRDHVCQREVAYA
                     HSVPRITSAIGSDED"
     polyA_signal    1516..1637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1644..2099)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2126..2230
                     /label=AmpR promoter
     promoter        2232..2589
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2624..3415
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3650..3697
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4026..4614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tgccaccatg tccgagggcg gccccgccct gttccagagc gacatgacct
      721 tcaaaatctt catcgacggc gaggtgaacg gccagaagtt caccatcgtg gccgacggca
      781 gcagcaagtt cccccacggc gacttcaacg tgcacgccgt gtgcgagacc ggcaagctgc
      841 ccatgagctg gaagcccatc tgccacctga tccagtacgg cgagcccttc ttcgcccgct
      901 accccgacgg catcagccat ttcgcccagg agtgcttccc cgagggcctg agcatcgacc
      961 gcaccgtgcg cttcgagaac gacggcacca tgaccagcca ccacacctac gagctggacg
     1021 acacctgcgt ggtgagccgc atcaccgtga actgcgacgg cttccagccc gacggcccca
     1081 tcatgcgcga ccagctggtg gacatcctgc ccaacgagac ccacatgttc ccccacggcc
     1141 ccaacgccgt gcgccagctg gccttcatcg gcttcaccac cgccgacggc ggcctgatga
     1201 tgggccactt cgacagcaag atgaccttca acggcagccg cgccatcgag atccccggcc
     1261 cacacttcgt gaccatcatc accaagcaga tgagggacac cagcgacaag cgcgaccacg
     1321 tgtgccagcg cgaggtggcc tacgcccaca gcgtgccccg catcaccagc gccatcggta
     1381 gcgacgagga ttaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     1441 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     1501 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1561 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1621 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     1681 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1741 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1801 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1861 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1921 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1981 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     2041 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     2101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2221 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     2281 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2341 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2401 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2461 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2521 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2581 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     2641 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     2701 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     2761 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     2821 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     2881 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     2941 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3001 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3061 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3121 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     3181 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3241 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3301 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3361 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     3421 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     3481 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     3541 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     3601 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     3661 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     3721 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     3781 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     3841 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     3901 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4141 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4621 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4681 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
