LOCUS       62056_7225        5874 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(88..143)
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(215..922)
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     intron          complement(1041..2058)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     promoter        2718..2921
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2986..3051
                     /codon_start=1
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    3175..3296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3325..3429
                     /label=AmpR promoter
     promoter        3431..3788
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3823..4614
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4849..4896
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5225..5813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagcatatgg gcatatgttg ccaaactcta aaccaaatac tcattctgat gttttaaatg
       61 atttgccctc ccatatgtcc ttccgagtga gagacacaaa aaattccaac acactattgc
      121 aatgaaaata aatttccttt attagccaga agtcagatgc tcaaggggct tcatgatgtc
      181 cccataattt ttggcagagg gaaaaagatc tctacttgta cagctcgtcc atgccgccgg
      241 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
      301 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
      361 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
      421 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
      481 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
      541 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
      601 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
      661 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
      721 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
      781 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
      841 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
      901 cctcctcgcc cttgctcacc atgagctccc accttctgat aggaagcttc agcctgcacc
      961 tgctagcgag gagtgaattc ctcgaggaat tctttgccaa aatgatgaga cagcacaata
     1021 accagcacgt tgcccaggag ctgtaggaaa aagaagaagg catgaacatg gttagcagag
     1081 gctctagagc cgccggtcac acgccagaag ccgaaccccg ccctgccccg tcccccccga
     1141 aggcagccgt ccccccgcgg acagccccga ggctggagag ggagaagggg acggcggcgc
     1201 ggcgacgcac gaaggccctc cccgcccatt tccttcctgc cggcgccgca ccgcttcgcc
     1261 ccgcgcccgc tagagggggt gcggcggcgc ctcccagatt tcggctccgc acagatttgg
     1321 gacaaaggaa gtccctgcgc cctctcgcac gattaccata aaaggcaatg gctgcggctc
     1381 gccgcgcctc gacagccgcc ggcgctccgg gggccgccgc gccccttccc ccgagccctc
     1441 cccggcccga ggcggccccg ccccgcccgg cacccccacc tgccgccacc ccccgcccgg
     1501 cacggcgagc cccgcgccac gccccgtacg gagccccgca cccgaagccg ggccgtgctc
     1561 agcaactcgg ggaggggggt gcaggggggg ttgcagcccg accgacgcgc ccacaccccc
     1621 tgctcacccc cccacgcaca caccccgcac gcagcctttg ttcccctcgc agcccccccc
     1681 gcaccgcggg gcaccgcccc cggccgcgct cccctcgcgc acactgcgga gcgcacaaag
     1741 ccccgcgccg cgcccgcagc gctcacagcc gccgggcagc gcggagccgc acgcggcgct
     1801 ccccacgcac acacacacgc acgcaccccc cgagccgctc cccccgcaca aagggccctc
     1861 ccggagcccc tcaaggcttt cacgcagcca cagaaaagaa acaagccgtc attaaaccaa
     1921 gcgctaatta cagcccggag gagaagggcc gtcccgcccg ctcacctgtg ggagtaacgc
     1981 ggtcagtcag agccggggcg ggcggcgcga ggcggcggcg gagcggggca cggggggaag
     2041 gcagcgcgca acgactcccg cccgccgcgc gcttcgcttt ttatagggcc gccgccgccg
     2101 ccgcctcgcc ataaaaggaa actttcggag cgcgccgctc tgattggctg ccgccgcacc
     2161 tttccgcctc gccccgcccc gcccctcgcc ccgccccgcc ccgcctgggg ggggcccccc
     2221 cccccccccc gcccccatcg ctgcacaaaa taattaaaaa ataaataaat acaaaattgg
     2281 gggtggggag gggggggaga tggggagagt gaagcagaac gtggggctca cctcgaccat
     2341 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     2401 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     2461 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     2521 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     2581 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     2641 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     2701 agtcaataat caatgtcgtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2761 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2821 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2881 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     2941 tccctgcagt cgacggtacc gcgggcccgg gatcctcagg atatcgacta caaagaccat
     3001 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     3061 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3121 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaagca
     3301 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3361 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3421 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     3481 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3541 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3601 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3661 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3721 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3781 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     3841 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     3901 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     3961 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     4021 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     4081 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     4141 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     4201 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     4261 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     4321 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     4381 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     4441 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     4501 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     4561 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     4621 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     4681 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     4741 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     4801 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     4861 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     4921 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     4981 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     5041 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     5101 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5161 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5221 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5281 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5341 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5401 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5461 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5521 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5581 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5641 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5701 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5761 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5821 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
