LOCUS       V026679                 7168 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026679
VERSION     V026679
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..2218
                     /label="Kcna3(NM_008418)"
                     /note="Kcna3(NM_008418)"
                     /gene="Kcna3"
     CDS             2231..2296
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    2337..2561
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        2577..3100
                     /label="mCMV promoter"
     CDS             3114..3833
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3956..4077
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4084..4539)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4566..4670
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4672..5029
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5064..5858
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6090..6137
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6466..7054
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatgacc gtggtgcccg gggaccacct
      661 gctggagcca gaggcggcgg gaggcggtgg cggggacccg cctcagggag gctgtggcag
      721 tggcggcggc ggtggcggct gcgaccgcta cgagccactg ccacccgcgc tgcccgccgc
      781 gggcgagcaa gattgctgcg gcgagcgtgt ggtcatcaac atctctgggc tgcgcttcga
      841 gacgcagctc aagaccctct gccagttccc cgagacactg ctgggcgacc ccaagcggcg
      901 catgcggtac tttgacccac tccgcaatga gtacttcttc gaccgcaacc gacccagctt
      961 cgacgccatc ctctactact accagtccgg gggccgcatt cgccggccgg tcaacgtgcc
     1021 catcgacatc ttctccgagg agatccgctt ttaccagctg ggtgaggagg ccatggaaaa
     1081 gttccgtgag gatgagggct tcctgcggga ggaggagcga cccctgcccc gccgtgactt
     1141 ccagcgccag gtgtggctgc tcttcgaata tccggagagc tccgggccgg cccggggcat
     1201 tgccattgtg tcagtgctgg tcattctcat ctccattgtc atcttctgct tggagacgct
     1261 tcccgagttt cgcgatgaga aagactatcc cgcctccccg tcgcaggacg tgtttgaggc
     1321 tgccaacaac agcacgtcgg gggccccttc tggagcctcc agcttctcgg accccttctt
     1381 cgtggtggag accttgtgca tcatctggtt ctcctttgag cttctggtgc ggttctttgc
     1441 ttgccccagt aaagccacct tctccagaaa tatcatgaac ttgatagaca ttgtggccat
     1501 cattccttat tttatcactc tgggcactga gctggctgaa cgacaaggta atgggcagca
     1561 ggccatgtcg ctggccatcc tgagagtcat ccgcctagta agggttttcc gcatcttcaa
     1621 gctctcccgc cattctaagg ggctgcagat cctaggacag acgctgaagg cttccatgcg
     1681 ggagctgggg ctgctcatat tcttcctctt cattggggtc atccttttct ccagtgcagt
     1741 ctactttgct gaggcagacg acccttcttc gggttttaac agtatcccgg atgccttctg
     1801 gtgggcagta gtaaccatga caactgttgg ttatggtgat atgcacccag tgaccatagg
     1861 aggcaagatt gtgggctctc tttgtgccat cgcaggtgtc ttgaccattg cattgccagt
     1921 tcctgtgatt gtttccaact tcaactactt ctaccaccgg gagacagaag gggaagagca
     1981 agcccagtac atgcacgtgg gcagttgcca gcacctctcc tcttcagccg aggagctccg
     2041 aaaagcccgg agtaactcca ctctgagtaa gtcggagtat atggtgatcg aagagggggg
     2101 tatgaaccac agcgccttcc cgcagacccc cttcaaaacg ggcaactcca cagccacttg
     2161 caccacgaac aataacccca actcctgtgt caacatcaag aagatattca ctgatgtcaa
     2221 gcttacgcgt gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     2281 ggatgacgat gacaagtgag gatccgggcc cgtttaaacc cgctgatcag cctcgactgt
     2341 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     2401 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2461 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     2521 agacaatagc aggcatgctg gggatgcggt gggctctatg gtctagagat atactgagtc
     2581 attagggact ttccaatggg ttttgcccag tacataaggt caataggggt gaatcaacag
     2641 gaaagtccca ttggagccaa gtacactgag tcaataggga ctttccattg ggttttgccc
     2701 agtacaaaag gtcaataggg ggtgagtcaa tgggtttttc ccattattgg cacgtacata
     2761 aggtcaatag gggtgagtca ttgggttttt ccagccaatt taattaaaac gccatgtact
     2821 ttcccaccat tgacgtcaat gggctattga aactaatgca acgtgacctt taaacggtac
     2881 tttcccatag ctgattaatg ggaaagtacc gttctcgagc caatacacgt caatgggaag
     2941 tgaaagggca gccaaaacgt aacaccgccc cggttttccc ctggaaattc catattggca
     3001 cgcattctat tggctgagct gcgttctacg tgggtataag aggcgcgacc agcgtcggta
     3061 ccgtcgcagt cttcggtctg accaccgtag aacgcagatc accggtcgcc accatggtga
     3121 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3181 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3241 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3301 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     3361 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3421 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3481 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3541 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3601 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3661 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3721 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3781 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     3841 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3901 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3961 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4021 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     4081 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     4141 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     4201 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     4261 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4321 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4381 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4441 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4501 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     4561 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4621 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     4681 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4741 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4801 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4861 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4921 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4981 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     5041 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     5101 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5161 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5221 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     5281 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5341 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5401 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5461 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5521 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5581 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5641 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5701 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5761 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5821 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5881 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5941 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6001 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     6061 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     6121 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     6181 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     6241 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     6301 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6361 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6601 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7081 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7141 tgtggataac cgtattaccg ccatgcat
//
