LOCUS       pCMV-KPNA3(human)-HA    5318 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035792
VERSION     V035792
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             844..2406
                     /label="KPNA3(NM_002267)"
                     /note="KPNA3(NM_002267)"
                     /gene="KPNA3"
     CDS             2416..2442
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    2457..2591
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2702..2718)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2726..2742
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2750..2780)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2795..2816
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3104..3692)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3863..4723)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4724..4828)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5302..5318
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcgcc
      841 accatggccg agaaccccag cttggagaac caccgcatca agagcttcaa gaacaagggc
      901 cgcgatgtgg aaacaatgcg aagacataga aatgaagtga cagtggaact gcggaagaac
      961 aaaagagatg aacacttatt gaaaaagaga aatgttcccc aagaagaaag tctagaagat
     1021 tcagatgttg atgctgattt taaagcacaa aatgtaaccc tagaagctat attgcagaat
     1081 gccacaagtg ataacccagt ggtccaattg agtgctgtcc aggcagcaag aaaactgtta
     1141 tccagtgaca gaaatccacc gattgatgac ttaataaaat ctgggatttt accaattcta
     1201 gtcaaatgtc tagaaaggga tgataatcct tcattacagt ttgaagctgc ttgggcatta
     1261 actaacatag catcaggaac ttctgcacag actcaagctg ttgtgcagtc taatgcagta
     1321 cctctttttc tgagacttct tcgttcacca catcagaatg tttgtgaaca agcagtatgg
     1381 gctttgggaa acattatagg tgatggtcct caatgtagag attatgtcat atcactggga
     1441 gttgtcaaac ctcttctgtc cttcatcagt ccctccatcc ccatcacctt ccttcggaac
     1501 gtcacatggg tcattgtcaa tctctgcagg aataaggatc ccccgccgcc tatggagaca
     1561 gttcaggaga ttttgccagc tttatgtgtc ctcatatacc atacagatat aaacattctt
     1621 gtagacactg tttgggctct gtcatacttg acagatggag gtaatgaaca gatacagatg
     1681 gttattgatt caggagttgt gccctttctt gtgccccttc tgagccatca ggaagtcaaa
     1741 gttcaaacag cagccctcag agcagttggc aacatagtga ctggcaccga cgagcagacc
     1801 caggttgttc tcaattgtga tgtcctgtca cacttcccaa atctcttatc acacccaaaa
     1861 gagaagataa ataaggaagc agtgtggttc ctttccaaca taacagcagg caaccagcaa
     1921 caagttcaag ctgtaataga tgctggatta attcctatga taattcatca gcttgctaag
     1981 ggggactttg gaacacaaaa agaagctgct tgggcaatca gcaacttaac aataagtggc
     2041 agaaaagatc aggttgagta ccttgtacag cagaatgtaa taccaccgtt ctgtaattta
     2101 ctgtcagtga aagattctca agtggttcag gtggttctag atggtctaaa aaacattctg
     2161 ataatggccg gtgatgaagc aagcacaata gctgaaataa tagaggaatg tggaggtttg
     2221 gagaaaattg aagttttaca gcaacatgaa aatgaagaca tatataaatt agcatttgaa
     2281 atcatagatc agtatttctc tggtgatgat attgatgaag atccctgcct cattcctgaa
     2341 gcaacacaag gaggtaccta caattttgat ccaacagcca accttcaaac aaaagaattt
     2401 aattttggta ccggctaccc atacgatgtt ccagattacg cttaagcggc cgcggggatc
     2461 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     2521 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     2581 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     2641 gggaggtttt ttcggatcct ctagagtcga tctgcaggca tgctagcttg gcgtaatcat
     2701 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     2761 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     2821 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     2881 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2941 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3541 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3841 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3901 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3961 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4021 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4081 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4141 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4201 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4261 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4321 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4381 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4441 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4501 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4561 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4621 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4681 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4741 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4801 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     4861 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     4921 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4981 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     5041 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     5101 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     5161 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     5221 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     5281 ggttttccca gtcacgacgt tgtaaaacga cggccagt
//
