LOCUS       V035845                 5576 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035845
VERSION     V035845
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             844..2661
                     /label="KLHL12(NM_001303051)"
                     /note="KLHL12(NM_001303051)"
                     /gene="KLHL12"
     CDS             2671..2700
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    2715..2849
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2960..2976)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2984..3000
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3008..3038)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3053..3074
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3362..3950)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4121..4981)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4982..5086)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5560..5576
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcgcc
      841 accatggacg tgaataaatt tgaggcctct gtgggttttc tcgatgtcaa aaagttcctc
      901 agtacctgga agttgcagaa tccaaggacc cattttgttc tttctccgca ctgctttatg
      961 ggaggcatta tggcccccaa agacataatg acaaatactc atgctaaatc catcctcaat
     1021 tcaatgaact ccctcaggaa gagcaatacc ctctgtgatg tgacattgag agtagagcag
     1081 aaagacttcc ctgcccatcg gattgtgctg gctgcctgta gtgattactt ctgtgccatg
     1141 ttcactagtg agctctcaga gaaggggaaa ccttatgttg acatccaagg tttgactgcc
     1201 tctaccatgg aaattttatt ggactttgtg tacacagaaa cagtacatgt gacagtggag
     1261 aatgtacaag aactgcttcc tgcagcctgt ctgcttcagt tgaaaggtgt gaaacaagcc
     1321 tgctgtgagt tcttagaaag tcagttggac ccttctaatt gcctgggtat tagggatttt
     1381 gctgaaaccc acaattgtgt tgacctgatg caagcagctg aggtttttag ccagaagcat
     1441 tttcctgaag tggtacagca tgaagagttc attcttctga gtcaaggaga ggtggaaaag
     1501 ctaatcaagt gcgacgaaat tcaggtggat tctgaagagc cagtctttga ggctgtcatc
     1561 aactgggtga agcatgccaa gaaagagcgg gaagaatcct tgcctaacct gctacagtat
     1621 gtgcggatgc ccctactaac ccccaggtat atcacagatg taatagatgc tgagcctttc
     1681 atccgctgta gtttacaatg cagggatctg gttgatgaag caaagaagtt tcatctgagg
     1741 cctgaacttc ggagtcagat gcagggaccc aggacaaggg ctcgcctagg agccaatgaa
     1801 gtgcttttgg tggttggggg ctttggaagc cagcagtctc ccattgatgt ggtagagaaa
     1861 tatgacccca agactcagga gtggagcttt ttgccaagca tcactcgtaa gagacgttat
     1921 gtggcctcag tgtcccttca tgaccggatc tacgtcattg gtggctatga tggccgttcc
     1981 cgccttagtt cagtggaatg tctagactac acagcagatg aggatggggt ctggtattct
     2041 gtggccccta tgaatgtccg acgaggtctt gctggagcca ccaccctggg agatatgatc
     2101 tatgtctctg gaggctttga tggaagcagg cgtcacacca gtatggagcg ctatgatcca
     2161 aacattgacc agtggagcat gctgggagat atgcagacag cccgggaagg tgccggactc
     2221 gtagtggcca gtggagtgat ctactgtcta ggaggatatg acggcttgaa tatcttaaat
     2281 tcagttgaga aatacgaccc tcatacagga cattggacta atgttacacc aatggccacc
     2341 aagcgttctg gtgcaggagt agccctgctg aatgaccata tttatgtggt ggggggattt
     2401 gatggtacag cccacctttc ttccgttgaa gcatacaaca ttcgcactga ttcctggaca
     2461 actgtcacca gtatgaccac tccacgatgc tatgtagggg ccacagtgct tcgggggaga
     2521 ctctatgcaa ttgcaggata tgatggtaat tccctgctaa gtagcattga atgttatgac
     2581 cctatcatcg acagctggga agtcgtgaca tccatgggaa cccagcgctg tgatgctggt
     2641 gtttgtgttc tccgcgagaa gggtaccggc gagcagaagc tgatctcaga ggaggacctg
     2701 taagcggccg cggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     2761 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2821 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     2881 aggttcaggg ggaggtgtgg gaggtttttt cggatcctct agagtcgatc tgcaggcatg
     2941 ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3001 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3061 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3121 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3181 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3241 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3301 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3361 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3421 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3481 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3541 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3601 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3661 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3721 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3781 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3841 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3901 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3961 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4021 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4081 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4141 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4201 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4261 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4321 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4381 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4441 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4501 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4561 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4621 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4681 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4741 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4801 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4861 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4921 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4981 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5041 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5101 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5161 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     5221 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     5281 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     5341 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     5401 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     5461 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     5521 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagt
//
