LOCUS       .                       9718 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     polyA_signal    163..297
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(303..321)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(335..351)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(359..375)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(383..413)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(428..449)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(737..1325)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1499..2356)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(2357..2461)
                     /label="AmpR promoter"
     rep_origin      2488..2943
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3084..3100
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     enhancer        3261..3640
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3641..3844
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             7344..7409
                     /label="3xFLAG"
                     /note="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
     polyA_signal    7420..8042
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     promoter        8084..8381
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             8435..9226
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gccaagctgg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgccgatc
       61 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      121 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaat
      301 tccctatagt gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
      361 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      421 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      481 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      541 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1141 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1381 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1441 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1501 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1561 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1621 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1681 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1741 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1801 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2101 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2461 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     2521 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2581 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2641 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2701 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2761 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2821 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2881 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     2941 tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3001 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     3061 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga tctatacatt
     3121 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
     3181 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
     3241 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     3301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
     3541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
     3601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     3661 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     3721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
     3781 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     3841 agctcgttta gtgaaccgtc agaattaagc ttgccaccat gccacgacta actgcgatgg
     3901 aactgagccg gcagctgtgc attaagatgc ggaagccgaa gaggagtgag accgccctgc
     3961 ctgttgccag atggggcctg gagatccact tctatctgcc tgtggccaac cctccggagt
     4021 acctgatggg ctgctactcg gctgaggacc tgtgcagcga ggctgccaag aagtgcggta
     4081 tttctcctct ctgtcacaat ttatttgctc tctacgacga gagcaggtgc atctggtacc
     4141 cgcccaatca cgctttcaaa atcgacgaca gcaccagcat caagctgtac taccgcatga
     4201 ggtattactt caccaactgg catggtacga acgcagatga gtccccagtg tggcggcact
     4261 gcctgaacaa gcagaagacc tgccccacaa aggccccaga gggcacccca ctcctggacg
     4321 ctgcctcgct cgagtaccta tttgcacagg ggcagtacga tttcctgagg ggcctggctc
     4381 ccgtgcggaa tgcagtaagc gagtcagaga tccacgacat tgagaacgag tgcctgggca
     4441 tggccgtgct cgctatttcc cactacgcta tcagcagaaa actgtccctg cccgctgttg
     4501 ctagggagat cagctacaaa aaatttattc cagagagtct gaacagaacc atcaagcagc
     4561 gcaacttcct cacccgcatc cgcatcaaca acgtcttcag gaactttgtc aatgaatttg
     4621 acagcaaaac catccgcgat agcaacgtat ccacgcatga tctgaaggtg aagtacctgt
     4681 ccacgctgga gaccctaacc aggaactttg gcagtgaggt gtacgagaca aacatgctga
     4741 agatctctca ggagagcgag aggccatcgg tcttcgactg tgtcgacccc tgcgtttacg
     4801 aggtgcaggc ctcggggact ggcggtatca aatggcggaa gaagccaccc aataatgtat
     4861 tgctgcccaa agagaagacc aaatccagaa agaacaaggt ggatggaaag agaaagaatg
     4921 agaaaaagga agtcacggac agctggacgc gattttccga tttccatgaa atcacccacg
     4981 ttgtcatcaa ggagtccaca gtcaccattt acaaacaaga caacaagaag atggagctgc
     5041 agctggactt cccggccgag gcgctgtcct ttgcctctct ggtggacggg tacttccggc
     5101 tgacagtgga cgcccaccac tacctgtgca gcgaggtggc accatcctca gtggtgcaga
     5161 acctgcagaa cggctgccat ggtcccatat gcacggatta cgccatccac aaactgcgtc
     5221 aggggggtaa cgaggagggc atgtatgtgc tgcgctggag ctgcaccgac tacaaccaca
     5281 tcctcatgac tgtggcctgt aacgagctgg acctgagcga gtccaagcag agccggcagt
     5341 acaagaactt ccagattgag gtggatgcac ggggctacaa gctctgcggg acagagtcct
     5401 actggctgtc tctaaaggat ctgatggagc acctgtccgg acaatgcctg cgcacggaca
     5461 acgtcagctt ctacctcaag aggtgctgcc cacctgaacc gcgagaaatc tccaacctcc
     5521 tggtagtgac caataacaga gtggtggaat caccccctgc agatatcggt cagctgagct
     5581 tccacaggat tctcaaagaa gacatagtcg aggcggagca cctcggccgt ggaaccagga
     5641 ccaacatcta cgcaggcgtg ctgaaggtga agagtgagga ggacgacggg tacagcgcct
     5701 gccaggacgt gaaggtggtc ctgaaggtgc tgggttccag tcacagagac atctccctgg
     5761 cattctttga gacggccagt atgatgcggc agatctccca caagcacatc gccttgctgt
     5821 atggagtctg cgtccgcgag ctggagaaca tcatggtgga ggagttcgtg cagctgggcc
     5881 cgctggacat cttcatgcgc gggcagcgct acttcctcac cacggcctgg aagttccagg
     5941 tggccaagca gctggccggc gccctgagct acctggagga taagaagctg gtccacgggt
     6001 acgtgtgcac caagaacatc ctgctggctc gggccggcct ggacgacgac agcggcccct
     6061 tcatcaagct gagcgacccg ggcatcccca tcaccgtgct gacccgcgag gagtgcgttt
     6121 cgaggatccc gtggatcgcc ccggagtgcg tcaggaacac caaggacctg agcgtggcgg
     6181 ccgacaagtg gagcttcgga accacgctgt gggagatctg ctatgacggg gaggtccccc
     6241 tcaaggacaa gaagctcgcc gagaaggaga tgttctacgc taaagagtgt gagctggcca
     6301 ccccagactg caaagagctg gcagatctca tgagccagtg catgaactat gactgccgaa
     6361 agaggccctt cttcagagcc atagtgaggg acctcaacaa gctggaggaa cagaacccct
     6421 ccatcgtctc tggcatgatg ccacctcagg aagtggaccc caccgtgttt gagaaaaggt
     6481 tcctgaagaa gattcgagat ctgggagagg gtcactttgg caaggtggag ctgtgccggt
     6541 atgacccgcg gggcgaccgg accggcgagt tcgtggccgt caagtccctg aagccggaga
     6601 acaaggagga ccagagcagc aacctgtggc gcgagatcga catcctgagg gggctgtacc
     6661 acgacaacat cgtcaagtac atgggcatct gcagcgagga gggtggaacc tcgattaagc
     6721 tgatcatgga gtacctacct gctggtagtc tgaaggagta cctgccccgg tacaaggccc
     6781 agacggacct gaagaggctc ctgagttacg gagtccagat atgccagggt atggattacc
     6841 ttgggtcgca gaactatatc caccgggact tggcagcccg gaacgtgctt gtggagaacg
     6901 agaacacggt aaagatcggt gactttgggc tgaccaagag catcaaggac aacgagggct
     6961 attacaccgt gaaagatgac ctggacagcc ctgtcttctg gtatgcccca gagtgcctcg
     7021 tccactgcaa gttctaccgt gcatctgacg tgtggtcctt cggtgtcacc atgtacgagc
     7081 tgctgaccta ctgtgagaca gagtgcagcc caatggcggt gttcttgaag atgattggtc
     7141 gaacccacgg gcagatgaca gtgaccagac tggtgaaagt gctggaggag gggcagaggc
     7201 tgccccgtcc agccaactgc ccagaaatgg tttacaccca gatgcagaaa tgctgggaga
     7261 atgctccgga gaagaggatc gacttcaaaa gcctgatcag gatattccag cagctgattg
     7321 ccgagaaacc gggatcccgg gctgactaca aagaccatga cggtgattat aaagatcatg
     7381 acatcgacta caaggatgac gatgacaagt agtgatcccg ggtggcatcc ctgtgacccc
     7441 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     7501 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     7561 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     7621 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     7681 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     7741 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     7801 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     7861 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     7921 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     7981 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     8041 ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     8101 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     8161 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     8221 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8281 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8341 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggaattga tcagcttggg
     8401 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     8461 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     8521 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     8581 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     8641 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     8701 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     8761 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     8821 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     8881 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     8941 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     9001 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     9061 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     9121 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     9181 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     9241 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     9301 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     9361 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccgggctcga tcccctcgcg
     9421 agttggttca gctgctgcct gaggctggac gacctcgcgg agttctaccg gcagtgcaaa
     9481 tccgtcggca tccaggaaac cagcagcggc tatccgcgca tccatgcccc cgaactgcag
     9541 gagtggggag gcacgatggc cgctttggtc gacccggacg ggacgctcct gcgcctgata
     9601 cagaacgaat tgcttgcagg catctcatga gtgtgtcttc ccgttttccg cctgaggtca
     9661 ctgcgtggat ggagcgctgg cgcctgctgc gcgacggcga gctgctcacc acccactc
//
