LOCUS       V013148                11286 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013148
VERSION     V013148
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4057..4110
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             4123..6486
                     /gene="ITGB3"
                     /label="Integrin beta-3"
                     /note="Integrin beta-3 from Homo sapiens. Accession#:
                     P05106"
     intron          6572..6801
                     /label="chimeric intron"
                     /note="chimera between introns from adenovirus and
                     immunoglobulin heavy chain genes"
     misc_feature    6869..7456
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             7482..8498
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    8907..9028
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(9177..9207)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9222..9243)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9531..10119)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10293..11150)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11151..11255)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ctgtacaggc cttaagccgg cgcgctagca tggccagagc tttgtgtcca
      961 ctgcaagccc tctggcttct ggagtgggtg ctgctgctct tgggaccttg tgctgcccct
     1021 ccagcctggg ccttgaacct ggacccagtg cagctcacct tctatgcagg ccccaatggc
     1081 agccagtttg gattttcact ggacttccac aaggacagcc atgggagagt ggccatcgtg
     1141 gtgggcgccc cgcggaccct gggccccagc caggaggaga cgggcggcgt gttcctgtgc
     1201 ccctggaggg ccgagggcgg ccagtgcccc tcgctgctct ttgacctccg tgatgagacc
     1261 cgaaatgtag gctcccaaac tttacaaacc ttcaaggccc gccaaggact gggggcgtcg
     1321 gtcgtcagct ggagcgacgt cattgtggcc tgcgccccct ggcagcactg gaacgtccta
     1381 gaaaagactg aggaggctga gaagacgccc gtaggtagct gctttttggc tcagccagag
     1441 agcggccgcc gcgccgagta ctccccctgt cgcgggaaca ccctgagccg catttacgtg
     1501 gaaaatgatt ttagctggga caagcgttac tgtgaagcgg gcttcagctc cgtggtcact
     1561 caggccggag agctggtgct tggggctcct ggcggctatt atttcttagg tctcctggcc
     1621 caggctccag ttgcggatat tttctcgagt taccgcccag gcatcctttt gtggcacgtg
     1681 tcctcccaga gcctctcctt tgactccagc aacccagagt acttcgacgg ctactggggg
     1741 tactcggtgg ccgtgggcga gttcgacggg gatctcaaca ctacagaata tgtcgtcggt
     1801 gcccccactt ggagctggac cctgggagcg gtggaaattt tggattccta ctaccagagg
     1861 ctgcatcggc tgcgcggaga gcagatggcg tcgtattttg ggcattcagt ggctgtcact
     1921 gacgtcaacg gggatgggag gcatgatctg ctggtgggcg ctccactgta tatggagagc
     1981 cgggcagacc gaaaactggc cgaagtgggg cgtgtgtatt tgttcctgca gccgcgaggc
     2041 ccccacgcgc tgggtgcccc cagcctcctg ctgactggca cacagctcta tgggcgattc
     2101 ggctctgcca tcgcacccct gggcgacctc gaccgggatg gctacaatga cattgcagtg
     2161 gctgccccct acgggggtcc cagtggccgg ggccaagtgc tggtgttcct gggtcagagt
     2221 gaggggctga ggtcacgtcc ctcccaggtc ctggacagcc ccttccccac aggctctgcc
     2281 tttggcttct cccttcgagg tgccgtagac atcgatgaca acggataccc agacctgatc
     2341 gtgggagctt acggggccaa ccaggtggct gtgtacagag ctcagccagt ggtgaaggcc
     2401 tctgtccagc tactggtgca agattcactg aatcctgctg tgaagagctg tgtcctacct
     2461 cagaccaaga cacccgtgag ctgcttcaac atccagatgt gtgttggagc cactgggcac
     2521 aacattcctc agaagctatc cctaaatgcc gagctgcagc tggaccggca gaagccccgc
     2581 cagggccggc gggtgctgct gctgggctct caacaggcag gcaccaccct gaacctggat
     2641 ctgggcggaa agcacagccc catctgccac accaccatgg ccttcctttg agatgaggca
     2701 gacttccggg acaagctgag ccccattgtg ctcagcctca atgtgtccct accgcccacg
     2761 gaggctggaa tggcccctgc tgtcgtgctg catggagaca cccatgtgca ggagcagaca
     2821 cgaatcgtcc tggactgtgg ggaagatgac gtatgtgtgc cccagcttca gctcactgcc
     2881 agcgtgacgg gctccccgct cctagttggg gcagataatg tcctggagct gcagatggac
     2941 gcagccaacg agggcgaggg ggcctatgaa gcagagctgg ccgtgcacct gccccagggc
     3001 gcccactaca tgcgggccct aagcaatgtc gagggctttg agagactcat ctgtaatcag
     3061 aagaaggaga atgagaccag ggtggtgctg tgtgagctgg gcaaccccat gaagaagaac
     3121 gcccagatag gaatcgcgat gttggtgagc gtggggaatc tggaagaggc tggggagtct
     3181 gtgtccttcc agctgcagat acggagcaag aacagccaga atccaaacag caagattgtg
     3241 ctgctggacg tgccggtccg ggcagaggcc caagtggagc tgcgagggaa ctcctttcca
     3301 gcctccctgg tggtggcagc agaagaaggt gagagggagc agaacagctt ggacagctgg
     3361 ggacccaaag tggagcacac ctatgagctc cacaacaatg gccctgggac tgtgaatggt
     3421 cttcacctca gcatccacct tccgggacag tcccagccct ccgacctgct ctacatcctg
     3481 gatatacagc cccagggggg ccttcagtgc ttcccacagc ctcctgtcaa ccctctcaag
     3541 gtggactggg ggctgcccat ccccagcccc tcccccattc acccggccca tcacaagcgg
     3601 gatcgcagac agatcttcct gccagagccc gagcagccct cgaggcttca ggatccagtt
     3661 ctcgtaagct gcgactcggc gccctgtact gtggtgcagt gtgacctgca ggagatggcg
     3721 cgcgggcagc gggccatggt cacggtgctg gccttcctgt ggctgcccag cctctaccag
     3781 aggcctctgg atcagtttgt gctgcagtcg cacgcatggt tcaacgtgtc ctccctcccc
     3841 tatgcggtgc ccccgctcag cctgccccga ggggaagctc aggtgtggac acagctgctc
     3901 cgggccttgg aggagagggc cattccaatc tggtgggtgc tggtgggtgt gctgggtggc
     3961 ctgctgctgc tcaccatcct ggtcctggcc atgtggaagg tcggcttctt caagcggaac
     4021 cggccacccc tggaagaaga tgatgaagag ggggaggagg gcagaggaag tcttctaaca
     4081 tgcggtgacg tggaggagaa tcccggccct gctagcgcca ccatgcgagc gcggccgcgg
     4141 ccccggccgc tctgggcgac tgtgctggcg ctgggggcgc tggcgggcgt tggcgtagga
     4201 gggcccaaca tctgtaccac gcgaggtgtg agctcctgcc agcagtgcct ggctgtgagc
     4261 cccatgtgtg cctggtgctc tgatgaggcc ctgcctctgg gctcacctcg ctgtgacctg
     4321 aaggagaatc tgctgaagga taactgtgcc ccagaatcca tcgagttccc agtgagtgag
     4381 gcccgagtac tagaggacag gcccctcagc gacaagggct ctggagacag ctcccaggtc
     4441 actcaagtca gtccccagag gattgcactc cggctccggc cagatgattc gaagaatttc
     4501 tccatccaag tgcggcaggt ggaggattac cctgtggaca tctactactt gatggacctg
     4561 tcttactcca tgaaggatga tctgtggagc atccagaacc tgggtaccaa gctggccacc
     4621 cagatgcgaa agctcaccag taacctgcgg attggcttcg gggcatttgt ggacaagcct
     4681 gtgtcaccat acatgtatat ctccccacca gaggccctcg aaaacccctg ctatgatatg
     4741 aagaccacct gcttgcccat gtttggctac aaacacgtgc tgacgctaac tgaccaggtg
     4801 acccgcttca atgaggaagt gaagaagcag agtgtgtcac ggaaccgaga tgccccagag
     4861 ggtggctttg atgccatcat gcaggctaca gtctgtgatg aaaagattgg ctggaggaat
     4921 gatgcatccc acttgctggt gtttaccact gatgccaaga ctcatatagc attggacgga
     4981 aggctggcag gcattgtcca gcctaatgac gggcagtgtc atgttggtag tgacaatcat
     5041 tactctgcct ccactaccat ggattatccc tctttggggc tgatgactga gaagctatcc
     5101 cagaaaaaca tcaatttgat ctttgcagtg actgaaaatg tagtcaatct ctatcagaac
     5161 tatagtgagc tcatcccagg gaccacagtt ggggttctgt ccatggattc cagcaatgtc
     5221 ctccagctca ttgttgatgc ttatgggaaa atccgttcta aagtagagct ggaagtgcgt
     5281 gacctccctg aagagttgtc tctatccttc aatgccacct gcctcaacaa tgaggtcatc
     5341 cctggcctca agtcttgtat gggactcaag attggagaca cggtgagctt cagcattgag
     5401 gccaaggtgc gaggctgtcc ccaggagaag gagaagtcct ttaccataaa gcccgtgggc
     5461 ttcaaggaca gcctgatcgt ccaggtcacc tttgattgtg actgtgcctg ccaggcccaa
     5521 gctgaaccta atagccatcg ctgcaacaat ggcaatggga cctttgagtg tggggtatgc
     5581 cgttgtgggc ctggctggct gggatcccag tgtgagtgct cagaggagga ctatcgccct
     5641 tcccagcagg acgaatgcag cccccgggag ggtcagcccg tctgcagcca gcggggcgag
     5701 tgcctctgtg gtcaatgtgt ctgccacagc agtgactttg gcaagatcac gggcaagtac
     5761 tgcgagtgtg acgacttctc ctgtgtccgc tacaaggggg agatgtgctc aggccatggc
     5821 cagtgcagct gtggggactg cctgtgtgac tccgactgga ccggctacta ctgcaactgt
     5881 accacgcgta ctgacacctg catgtccagc aatgggctgc tgtgcagcgg ccgcggcaag
     5941 tgtgaatgtg gcagctgtgt ctgtatccag ccgggctcct atggggacac ctgtgagaag
     6001 tgccccacct gcccagatgc ctgcaccttt aagaaagaat gtgtggagtg taagaagttt
     6061 gaccggggag ccctacatga cgaaaatacc tgcaaccgtt actgccgtga cgagattgag
     6121 tcagtgaaag agcttaagga cactggcaag gatgcagtga attgtaccta taagaatgag
     6181 gatgactgtg tcgtcagatt ccagtactat gaagattcta gtggaaagtc catcctgtat
     6241 gtggtagaag agccagagtg tcccaagggc cctgacatcc tggtggtcct gctctcagtg
     6301 atgggggcca ttctgctcat tggccttgcc gccctgctca tctggaaact cctcatcacc
     6361 atccacgacc gaaaagaatt cgctaaattt gaggaagaac gcgccagagc aaaatgggac
     6421 acagccaaca acccactgta taaagaggcc acgtctacct tcaccaatat cacgtaccgg
     6481 ggcacttaag gatccgatat cgtacgttaa catagataac tgatccagtg tgctggaatt
     6541 aattcgctgt ctgcgagggc cagctgttgg ggtgagtact ccctctcaaa agcgggcatg
     6601 acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc
     6661 gcggtgatgc ctttgagggt ggccgcgtcc atctggtcag aaaagacaat ctttttgttg
     6721 tcaagcttga ggtgtggcag gcttgagatc tggccataca cttgagtgac aatgacatcc
     6781 actttgcctt tctctccaca ggtgtccact cccaggtcca actgcaggtc gagcatgcat
     6841 ctagggcggc cgcactagag gaattccgcc cctctccctc ccccccccct aacgttactg
     6901 gccgaagccg cttggaataa ggccggtgtg tgtttgtcta tatgttattt tccaccatat
     6961 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     7021 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     7081 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     7141 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     7201 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     7261 aatggctctc ctcaagcgta gtcaacaagg ggctgaagga tgcccagaag gtaccccatt
     7321 gtatgggaat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     7381 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     7441 taagcttgcc acaacccgta ccaaagatgg atagatccgg aaagcctgaa ctcaccgcga
     7501 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     7561 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     7621 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     7681 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     7741 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     7801 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     7861 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     7921 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     7981 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     8041 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     8101 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     8161 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     8221 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     8281 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     8341 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     8401 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     8461 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gagtagatgc cgaccgaaca
     8521 agagctgatt tcgagaacgc ctcagccagc aactcgcgcg agcctagcaa ggcaaatgcg
     8581 agagaacggc cttacgcttg gtggcacagt tctcgtccac agttcgctaa gctcgctcgg
     8641 ctgggtcgcg ggagggccgg tcgcagtgat tcaggccctt ctggattgtg ttggtcccca
     8701 gggcacgatt gtcatgccca cgcactcggg tgatctgact gatcccgcag attggagatc
     8761 gccgcccgtg cctgccgatt gggtgcagat ctagataact gatcataatc agccatacca
     8821 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     8881 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     8941 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9001 gtttgtccaa actcatcaat gtatcttaac gctcgagtgc attctagttg tggtttgtcc
     9061 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     9121 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9181 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9241 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9301 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9721 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9961 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11281 gacgtc
//
